PhytoChem & BioSub Journal Peer-reviewed research journal on Phytochemistry & Bioactives Substances ISSN 2170 - 1768

PCBS Journal Volume 8 N° 1, 2 & 3

2014

PhytoChem & BioSub Journal ISSN 2170-1768

ISSN 2170 – 1768

PhytoChem & BioSub Journal (PCBS Journal) is a peer-reviewed research journal published by Phytochemistry & Organic Synthesis Laboratory. The PCBS Journal publishes innovative research papers, reviews, mini-reviews, short communications and technical notes that contribute significantly to further the scientific knowledge related to the field of Phytochemistry & Bioactives Substances (Medicinal Plants, Ethnopharmacology, Pharmacognosy, Phytochemistry, Natural products, Analytical Chemistry, Organic Synthesis, Medicinal Chemistry, Pharmaceutical Chemistry, Biochemistry, Computational Chemistry, Molecular Drug Design, Pharmaceutical Analysis, Pharmacy Practice, Quality Assurance, Microbiology, Bioactivity and Biotechnology of Pharmaceutical Interest ) It is essential that manuscripts submitted to PCBS Journal are subject to rapid peer review and are not previously published or under consideration for publication in another journal. Contributions in all areas at the interface of Chemistry, Pharmacy, Medicine and Biology are welcomed. Editor in Chief Pr Abdelkrim CHERITI Phytochemistry & Organic Synthesis Laboratory

Co-Editor Pr Nasser BELBOUKHARI Bioactive Molecules & Chiral Separation Laboratory University of Bechar, 08000, Bechar, Algeria Editorial Board Afaxantidis J. (France), Akkal S. (Algeria), Al Hamel M. (Morocco), Allouch A. (Lebanon), Aouf N. (Algeria), Asakawa Y. (Japan), Atmani A. (Morocco) , Awad Allah A.( Palestine), Azarkovitch M. ( Russia), Baalioumer A. (Algeria), Badjah A.Y. ( KSA), Balansard G. (France), Barkani M. (Algeria), Belkhiri A. (Algeria), Benachour D. (Algeria), Ben Ali Cherif N. (Algeria), Benayache F. (Algeria), Benayache S. (Algeria), Benharathe N. (Algeria), Benharref A. (Morocco), Bennaceur M. ( Algeria), Bensaid O. (Algeria), Berada M. ( Algeria), Bhalla A. ( India), Bnouham M. (Morocco), Bombarda E. (France), Bouchekara M. (Algeria), Boukebouz A. (Morocco), Boukir A. (Morocco), Bressy C. (France), Chehma A. (Algeria), Chemat F. (France), Chul Kang S. (Korea), Dadamoussa B. (Algeria), Daiche A. (France), Daoud K. ( Algeria), De la Guardia M. ( Brazilia), Dendoughi H. (Algeria), Derdour A. (Algeria), Djafri A. (Algeria), Djebar S. (Algeria), Djebli N.(Algeria), Dupuy N. (France), El Abed D. (Algeria), EL Achouri M. (Morocco), El Hatab M. (Algeria), El Omar F. (Lebanon), Ermel G. ( France), Esnault M. A. ( France), Govender P. (South Africa), Jouba M. (Turkey), Hacini S. (Algeria), Hadj Mahamed M. (Algeria), Halilat M. T. (Algeria), Hamed El Yahia A. ( KSA), Hamrouni A. ( Tunisia), Hania M. ( Palestine), Iqbal A. (Pakistan), Gaydou E. (France), Ghanmi M. (Morocco), Gharabli S. (Jordan), Gherraf N. ( Algeria), Ghezali S. (Algeria), Gouasmia A. (Algeria), Greche H. (Morocco), Kabouche Z. (Algeria), Kacimi S. (Algeria), Kajima J.M. (Algeria), Kaid-Harche M. (Algeria), Kessat A. (Morocco), Khelil-Oueld Hadj A. (Algeria), Lahreche M.B. (Algeria), Lanez T. (Algeria), Leghseir B. (Algeria), Mahiuo V. (France), Marongu B. ( Italia), Marouf A. (Algeria), Meddah B.( Morocco), Melhaoui A. ( Morocco), Merati N. (Algeria), Mesli A. ( Algeria), Mushfik M. ( India), Nefati M. (Tunisia), Ouahrani M. R. (Algeria), Oueld Hadj M.D. (Algeria), Pons J.M. ( France), Radi A. (Morocco), Rahmouni A. (Algeria), Reddy K.H. ( South Africa), Reza Moein M. (Iran), Rhouati S. (Algeria), Roussel C. (France), Saidi M. (Algeria), Salgueiro L.D (Portugal), Salvador J. A. (Spain), Seghni L. (Algeria), Sharma S. ( India), Sidiqi S. K. ( India), Souri E. ( Turkey), Tabcheh M. (Lebanon), Tabti B. (Algeria), Taleb S. (Algeria), Tazerouti F. (Algeria), Vantyune N. (France), Villemin D. (France), Yayli N. (Turkey), Youcefi M. (Algeria), Ziyyat A. (Morocco), Zouieche L. (Algeria), Zyoud A.H. (Palestine).

PhytoChem & BioSub Journal Peer-reviewed research journal on Phytochemistry & Bioactives Substances

ISSN 2170 - 1768

PCBS Journal

Volume 8 N° 3 POSL

2014

Edition LPSO Phytochemistry & Organic Synthesis Laboratory http://www.pcbsj.webs.com , Email: [email protected]

PhytoChem & BioSub Journal Vol. 8(3) 2014 ISSN 2170-1768 CAS-CODEN:PBJHB3

 

PhytoChem & BioSub Journal

2014 Vol. 8 No. 3

ISSN 2170-1768

Contents PhytoChem & BioSub Journal Vol. 8 N° 3 ZYOUD Ahed H. & HILAL Hikmat S.

Investigation of curcumin as sensitizer for anatase TiO2 nanoparticles in photodegradation of of phenazopyridine with visible light

127

BRAHMI F., BOULKBACHE-MAKHLOUF L., YALAOUI-GUELLAL D., CHIBANE M. & MADANI K.

Comparative study on the antioxidant effect of aqueous and ethanolic extracts of Mentha 138 pulegium L. grown at two different locations BOUGHRARA B. & LEGSEIR B.

Activité larvicide des extraits de quelques plantes du Parc National El Kala, Algérie

150

SIDALI L., BRADA M., FAUCONNIER M-L & LOGNAY G.

Composition chimique et activité antimicrobienne de l’huile essentielle de Thymus vulgaris du Nord d’Algérie

156

BOUZIDI N., BOURAHA I., SERIDI H., ZEKRI L., DAGHBOUCHE Y. & EL HATTAB M.

Dosage direct du cuminaldéhyde dans l’extrait de Cumin et d’anisaldéhyde dans l’huile essentielle d’Anis vert par spectrométrie IRTF

162

HEMMAL S., CHEIKH N., BELBOUKHARI N. & CHERITI A.

Chiral Separation by HPLC and Biological Evaluation of Some Heterocyclic Derivatives of Hesperetin

170

ZEGHADA F. Z., FASLA B., BENNACEUR M. & MAROUF A.

Allelopathic potential of some Algerian plants

177

REBIAI A., LANEZ T. & BELFAR M. L.

Determination of caffeic acid and gallic acid in Algerian bee pollen by an HPLC method  

190

OUIS N., HARIRI A. & EL ABED D.

Composition chimique de l’huile essentielle du Fenouil ( Foeniculum vulgare) de la région de Mascara

126

198

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Investigation of curcumin as sensitizer for anatase TiO2 nanoparticles in photodegradation of of phenazopyridine with visible light Ahed H. ZYOUD* & Hikmat S. HILAL SSERL, Department of Chemistry, An-Najah National University, Nablus, West Bank, Palestine Received: March 20, 2014; Accepted: 21 May, 2014 Corresponding author Email [email protected] and [email protected]

Copyright © 2014-POSL DOI:10.163.pcbsj/2014.8.3.127

Abstract. This work describes a photodegradation catalyst, for water organic contaminants in visible light,

based on curcumin (a natural dye) sensitized TiO2 (anatase) nanoparticles. Phenazopyridine (a pharmaceutically active gradient) was used as a contaminant. A 400 nm, and shorter, cut of filter was used to confirm only visible light was used in photodegradation process with no UV radiation. The catalyst system was characterized by electronic absorption spectroscopy and XRD. The particle size forthe catalyst nanaprticles was calculated using Scherrer equation and found to be ~45 nm in average. Different reaction parameters were studied, such as effect of contaminant concentration, amount of loaded catalyst, and pH value on the photodegradation rate. Turn number (T.N.) and quantum yield (Q.Y.) values were calculated for comparative assessment of the catalyst effeciency. The results show the ability of curcumin dyes to sensitize TiO2 anatase nanoparticles in photodegradation phenazopyridine under visible radiation.

Key words: Curcumine; sensitization; photo-degradation; phenizopyridine; Anatase TiO2.

1. Introduction Different catalyst systems were suggested for photo-degradation of organic contaminants in water, using ultra violet radiations [1-4]. Despite the advantages of such photocatalyst systems, they demand UV rather than the freely abundant visible light. Because only a small fraction of UV exists in the arriving solar radiation [5, 6], researchers focus their efforts to sensitize their catalyst systems on visible solar light[7-14]. The most commonly used semiconductor in photo-degradation processes is the nano-sized titanium oxide TiO2, by virtue of its stability to photo corrosion, low cost, abundance, safety and high photo-catalytic activity [4, 15-18]. Dye-sensitized TiO2 particles were investigated in both light-to-electricity conversions (Graetzel cells) and in photodegradation processes [13, 14]. Different types of dyes are known including ruthenium complexes, CdS nano-particles and others [11, 19-22]. Natural dyes extracted from plants were also widely reported as sensitizers for TiO2 particles in Graetzel cells. Examples of such dyes are chlorophyll derivatives, natural porphyrines, anthocyanins, and curcumin which exist in molecular form [23-28]. Unlike the case with solar cells, natural dyes have been used to a far lesser extent as sensitizers in water purification 127

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Comparative study on the antioxidant effect of aqueous and ethanolic extracts of Mentha pulegium L. grown at two different locations BRAHMI Fatiha a*, BOULKBACHE-MAKHLOUF Lila a, YALAOUI-GUELLAL Drifa a, CHIBANE Mohamed b & MADANI Khodir a

a

Laboratoire 3BS, Faculté des Sciences de la Nature et de la Vie Université A. Mira de Bejaia, Bejaia 06000, Algérie b Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre Centre Universitaire de Bouira 10000, Algérie       Received: February 09, 2014; Accepted: 25 May, 2014 Corresponding author Email [email protected]

Copyright © 2014-POSL DOI:10.163.pcbsj/2014.8.3.138    

Abstract. Aqueous and ethanolic extracts of Mentha pulegium L. (Lamiaceae) from two different locations Tizi-Ouzou and Bejaia in Algeria were examined in vitro. Phenolic compounds and antioxidant activity were analyzed. Measurement of total phenolic and total flavonoids contents of the extracts of M. pulegium were achieved by using Folin-Ciocalteu and chloride aluminium methods, respectively. The total phenolic content of water extract from Tizi-Ouzou location was found significantly higher (55.78  2.78 mg GAE/g DW). The flavonoids content was also higher in the extracts from this location for both ethanolic and water extracts (2.17  0.12; 2.04  0.03 mg QE/g DW, respectively). Antioxidant activities were assessed by five in vitro antioxidant assays. Results showed that the two M. pulegium provenances were significantly different according to their antioxidant activity. Indeed, aqueous extract issued from Bejaia plant exhibited stronger antioxidant activity. For instance, Bejaia provenance shoots showed lower IC50 value of 20.35 μg/ mL for H2O2 test. Key words: Pennyroyal extracts; Antioxydant activity; Bejaia location, Tizi-Ouzou location.    

1. Introduction Mentha pulegium L. (Algerian name: Fliou) is one of the most frequently used Algerian herb, its aerial part is considered effective in treating a wide range of disorders, mainly for the treatment of flatulent dyspepsia and intestinal colic due to its carminative and antispasmodic properties (Dellile, 2007). Its essential oil and dry parts have been traditionally used in medicine (digestive, liver and gallbladder disorders, amenorrhea, gout, colds, increased micturition, skin diseases and abortifacient), gastronomy (culinary herb), aromatherapy and cosmetics. Recent research reported its antioxidant and antimicrobial properties (Teixera et al., 2012). The occurrence of at least some of mentioned above diseases as well as aging processes may result from oxidative stress leading to a variety of alterations within the human organism caused by reactive oxygen species (ROS). ROS is a collective term used to include both the 138   

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Activité larvicide des extraits de quelques plantes du Parc National El Kala, Algérie Boudjema BOUGHRARA & Belgacem LEGSEIR Laboratoire de Synthèse Biocatalyse et Organique Université Badji Mokhtar, BP 12 Annaba 23000- Algérie Received: December 30, 2013; Accepted: April 29, 2014 Corresponding author Email [email protected]

Copyright © 2014-POSL DOI:10.163.pcbsj/2014.8.3.150    

Résume. Notre travail s’oriente vers l’activité larvicide des extraits ethanoliques des plantes suivantes : Garou,

Laurier rose, lavande, et le thym. La collecte des larves a été réalisée au niveau des eaux larvaires dans la région du parc national d’El Kala. A partir d’extrait ethanolique, on a préparé des différentes concentrations (o, 4℅- O, 6℅- 0,8℅- 1℅). Après un temps de contacte de 24h, on dénombre les larves mortes et vivantes et on calcule le pourcentage de mortalité. Les résultats des tests d’activités larvicides réalisés par les extraits des plantes indiquent une relation directe des pourcentages de mortalité des larves avec la concentration des extraits. Mots clés : PN El Kala, plantes, activité larvicide.

Larvicidal activity of some plants extracts from El Kala National Park, Algeria Abstract. Our work focuses on the larvicidal activity of ethanolic extracts of the following plants: Garou, Oleander, Lavender, and Thyme. The collection of larvae was carried out at the larval waters in the region of El Kala National Park. The larvae were exposed to different concentrations of ethanol extracts (0, 4℅ - 0, 6℅ - 0.8℅ - 1℅) after a contact time of 24 hours, there were dead and live larvae to calculate the percentage of mortality. The results of larvicidal activity realized by the plants extracts, indicates a direct relation between mortality percentage of larvae and the extracts concentrations.

Key Words: PN El Kala, plants, larvicidal activity  

  1. INTRODUCTION La plante est un organisme vivant, soumis aux variations de son environnement. Son évolution se traduit par des différenciations morphologiques qui ont été à l’ origine de la classification botanique, et par différenciations biochimiques [1]. En Algérie, l’utilisation des plantes occupe une place importante dans la vie des populations, étant donné la richesse floristique de notre pays. Le parc national d’El Kala (PNEK) est intégralement inclus dans la wilaya d’El Tarf dont il occupe un tiers de sa superficie soit 76430 ha, c’est une région de zones humides, très 150

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Composition chimique et activité antimicrobienne de l’huile essentielle de Thymus vulgaris du Nord d’Algérie L. SIDALI 1, M. BRADA 1, M-L FAUCONNIER 2 & G. LOGNAY 3 1

Laboratoire de Valorisation des Substances Naturelles, Université de Khemis-Miliana, Route de Theniet El Had, 44225, Algeria 2 Unité de Chimie Générale et Organique 3 Unité de Chimie Analytique Université de Liège, Gembloux Agro-Bio Tech, 2 Passage des Déportés, B-5030 Gembloux, Belgium

    Received: December 16, 2013; Accepted: April 23, 2014 Corresponding author Email [email protected]

Copyright © 2014-POSL DOI:10.163.pcbsj/2014.8.3.156    

Résumé. Ce travail vise à l’étude de la composition chimique et de l’activité antibactérienne et antifongique de

l’huile essentielle de Thymus vulgaris vis-à-vis de cinq microorganismes (S.P. Sterptococcus, staphylococcus aureus, E.coli, Klebscila et condidat albicans). La composition chimique de l’huile essentielle isolée par hydrodistillation des parties aériennes de Thymus vulgaris a été analysée par GC/MS. Vingt-cinq composés représentant 100% de la composition chimique globale. Les constituants majoritaires sont le carvacrol (55,2%),γ-terpinene (12,6%), p-cymene (9,3%), linalol (3,9%) et le α-terpinene (2,8%). L’huile essentielle extraite a montré une forte activité antibactérienne et antifongique due principalement à la richesse de cette essence en carvacrol connu pour son efficacité contre tous les microorganismes testés. Ce travail rentre dans le cadre plus global de la mise en valeur des plantes aromatiques et médicinales d’Algérie.

Mots clé : Thymus vulgaris, hydrodistillation, huile essentielle, activité antimicrobienne, carvacrol. Chemical composition and antimicrobial activity of Thymus vulgaris Essential Oils from North Algeria Abstract. Twenty five constituents were identified by GC MS analysis of essential loils from . Thymus

vulgaris. The main compounds identified were carvacrol (55,2%),γ-terpinene (12,6%), p-cymene (9,3%), linalol (3,9%) and α-terpinene (2,8%). The obtained Essential oils exhibited antibacterial and antifungal activities against S.P. Sterptococcus, staphylococcus aureus, E.coli, Klebscila and condidat albicans

Key Words: Thymus vulgaris, hydrodistilation, Essential oils, antimicrobial activity, carvacrol  

1. Introduction La littérature disponible avec des rapports concernant l’identification, la caractérisation et la détermination de la composition chimique, et les propriétés antimicrobiennes des huiles essentielles de l’espèce Thymus ainsi que leurs applications dans 156   

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Dosage direct du cuminaldéhyde dans l’extrait de Cumin et d’anisaldéhyde dans l’huile essentielle d’Anis vert par spectrométrie IRTF N. BOUZIDI 1,2, I. BOURAHA 1, H. SERIDI 1,3, L. ZEKRI 1, Y. DAGHBOUCHE1,2 & M. EL HATTAB 1*

1

Laboratoire de Chimie des Substances Naturelles et de BioMolécules (LCSN-BioM) Laboratoire de Plantes Médicinales et Aromatiques (LPMA) Université de Blida1, B. P. 270 Soumâa (Blida 09000). Algérie. 3 Laboratoire de Biologie Marine - Université des Sciences et technologie Houari Boumediene. Alger, Algérie 2

    Received: January 10, 2014; Accepted: April 28, 2014 Corresponding author M. EL HATTAB

Copyright © 2014-POSL DOI:10.163.pcbsj/2014.8.3.162  

Résumé. Le présent travail porte sur le dosage quantitatif du cuminaldéhyde dans l’extrait de cumin et

d’anisaldéhyde dans l’huile essentielle de l’anis vert par spectrométrie IRTF. Le taux de cuminaldéhyde dans l’extrait de cumin a été obtenu sans traitement préalable, la bande spécifique considérée est à 1097 cm-1 corrigée avec une ligne de base stabilisée entre 957 -1325 cm-1. Le taux de cuminaldéhyde obtenu par cette méthode est de 40.75%. Etant donné que le taux d’anisaldéhyde est faible dans l’huile essentielle d’anis vert, la détermination de ce dernier par spectrométrie IRTF a été effectuée après dilution de l’HE dans le solvant et ce, après enrichissement. En considérant la bande d’absorbance spécifique à 1216 cm-1 corrigée avec une ligne de base stabilisée entre 658 - 1792 cm-1, Le taux de l’anisaldéhyde dans l’anis vert est de 0.14%. Mots clé: cumin, anis vert, cuminaldéhyde, anisaldéhyde, spectrométrie IRTF, addition standard

Direct Determination of cuminaldehyde in Cumin and anisaldehyde in the essential oil of anise green by FTIR spectrometry Abstract. Analytical method was developed for the direct Determination of cuminaldehyde in Cumin and

anisaldehyde in the essential oil of green anise by using FTIR spectrometry. The determination of compounds was carried out by considering the specific band 1097 cm-1 corrected with a baseline established between 957 -1325 cm-1 for cuminaldéhyde ( rate in cumin 40.75%. ) and 1216 cm-1 corrected between 658 - 1792 cm-1 for anisaldéhyde ( rate in EO of green anise 0.14%.)

Key Words: cumin, green anise, cuminaldehyde, anisaldehyde, FTIR spectrometry, standard addition  

Introduction

Les plantes aromatiques et médicinales constituent une richesse naturelle très importante dont la valorisation demande une parfaite connaissance des propriétés à 162   

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Chiral Separation by HPLC and Biological Evaluation of Some Heterocyclic Derivatives of Hesperetin Samia HEMMAL a*, Nawel CHEIKH b, Nasser BELBOUKHARI b & Abdelkrim CHERITI a a

Phytochemistry and Organic Synthesis Laboratory Bioactive Molecules and Chiral Separation Laboratory University of Bechar, 08000, Algeria   b

  Received: December 24, 2013; Accepted: March 31, 2014 Corresponding author Email [email protected]

Copyright © 2014-POSL DOI:10.163.pcbsj/2014.8.3.170    

Abstract. The separation of enantiomers of great interest to the pharmaceutical, chemical and food industries. This work has focused on studying the properties of chiral HPLC separation of some mono-and bis-heterocyclic derivatives Hesperetin possessing a stereogenic center in position 2. The chiral separation of these molecules was performed by HPLC using two chiral stationary phases derived polysaccharides (Chiralcel OJ, Chiralpak IA) and various mobile phases (alkane / alcohol). The Chiralpak IA column showed a better enantioseparation using methanol alone as polar mobile phase. In addition, these compounds have undergone antibacterial tests. The majority of compounds have an inhibitory overlooked all the microorganisms tested power. The antioxidant activity overlooked the DPPH have been shown that these derivatives showed a very important activity of free radicals. Key

Words:

Hesperetin,

Heterocyclic

derivatives,

Enantioseparation,

DPPH,

β-carotene,

antimicrobial

 

    1. Introduction Much of the current research interest focuses on the study of chiral molecules such as flavanons. Due to their polyphenolic structure, these compounds have health-related properties, which are based on their antioxidant activity as well as anticancer, antiviral and anti-inflammatory activities [1, 2]. Among the different types of flavanones most studied, we are interested in this work on Hesperetin [5,7-dihydroxy-2-(3'-hydroxy-4'-methoxyphenyl) chroman-4-one] is a flavanone obtained by the hydrolysis acid of hesperidin.  Hesperetin and its glycoside is also mainly present in citrus fruits. The aglycone is less dominant in nature than the glycoside. The most widely distributed glycoside of hesperetin is hesperidin, which is conjugate with rhamnosyl-β-1,6-glucose. Hesperidin (hesperetin-7-rutinoside) is present in higher extents in lemons, limes, sweet oranges, tangerine and tangor species of citrus fruits [3]. The flavanone Hesperetin is a chiral molecule exists in two enantiomeric forms due to the presence of an asymmetric carbon at position 2 [4], and it shows significant reactivity centers allowing him heterocyclic derivative formation [5]. It has in its structure two important 170   

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Allelopathic potential of some Algerian plants Fatima Zohra ZEGHADA 1*, Badria FASLA 1, Malika BENNACEUR 1,2 & Abderrazak MAROUF 3

1

Laboratoire de Biochimie Végétale et des Substances Naturelles Faculté des Sciences de la nature et de la vie, Département de Biologie, Université d’Oran, B.P.1524, El. Menouar, 31000 Oran, Algérie. 2 Laboratoire de Recherche sur les Zones Arides Université des Sciences et de la Technologie Houari Boumediene, Alger, Algérie. 3 Centre Universitaire de Naama, Algérie

  Received: April 21, 2014; Accepted: June 02, 2014 Corresponding author Email [email protected]

Copyright © 2014-POSL DOI:10.163.pcbsj/2014.8.3.177    

Abstract. Allelopathic activity study of 15 plant’s aqueous extracts was evaluated on the germination and

growth of two experimental models, Lactuca sativa and Rhaphanus sativus. The seeds tested were germinated in petri dishes with increasing concentration: 0.25; 0.5 ; 0.75 and 1 % of plant extract. Seasonal variation of allelopathic activity of Tetraclinis articulata was studied. Fractionation assay by liquid-liquid partitioning was also undertaken for the same species. In parallel of this biological activity test, phytochemical screening of the main phytoconstituents was established by TLC with quantification of phenolics and flavonoïds contents. Inhibitory effects with variable intensities were observed on the germination and growth of L. sativa. Aqueous extract of T. articulata was exhibited the more inhibitory effect on L. sativa germination while aqueous extract of Peganum harmala showed the more growth inhibitory effect with all concentrations tested, for the two experimental models. Investigation of seasonal variation revealed that June and November samples of T. articulata presented the most important inhibitory effect on L. sativa germination. Phytochimical screening by TLC identified that these active extracts contain phenolic acids, flavonoïds, cardiotonic glycosids, sesquiterpens lactons and saponins. Phenolics and flavonoïds contents quantified by spectrophotometry were very important in some active extracts like Globularia alypum, Pistacia lentiscus, Acacia raddiana and Haloxylon scoparium.

Key Words: Allelopathic activity, aqueous extracts, Algerian medicinal plants, germination, growth, flavonoïds, phenolics, phytochemistry, seasonal variation

 

Introduction The secondary metabolites of the plants are famous since antiquity for their pharmacological properties. For a few decades, the man has also been interested in their other biological activities. In particular, these secondary compounds are often regarded as being a 177   

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Determination of caffeic acid and gallic acid in Algerian bee pollen by an HPLC method Abdelkerim REBIAI, Touhami LANEZ & Mohamed Lakhder BELFAR University of El Oued, VTRS Laboratory, B.P.789, 39000, El Oued, Algeria

  Received: December 03, 2013; Accepted: March 25, 2014 Corresponding author Email [email protected]

Copyright © 2014-POSL DOI:10.163.pcbsj/2014.8.3.190    

Abstract. Bee pollen is a fine powder-like material produced by flowering plants pollen, mixed with nectar and bee secretions and gathered by the honey-bees. Pollens are the male reproductive cells of flowers and bees' primary food source, containing concentrations of phytochemicals and nutrients and rich in secondary metabolites. It is known that bee pollen contains lipids, sugars, proteins, amino acids, flavonoid and phenolic acids such as caffeic acid and gallic acid. The aim of our study is to evaluate caffeic and gallic acids from different sorts of bee pollen from Algeria using an HPLC method. Analysis of caffeic acid, and gallic acid was realized with the chromatographic column VPODS RP18 type and using a gradient of mobile phase (Acetonitril- 0,1% acetic acid) and spectrophotometric detection. Caffeic acid in the range from 24.734 to 42.182 µg/g and gallic acid from 0.321 to 0.406 mg/g was determined in tested pollen samples. Key Words: Bee pollen, HPLC, phenolic acids, caffeic acid, gallic acid.  

  INTRODUCTION Bee pollen has been used for many years in both traditional medicine and supplementary nutrition, as well as in alternative diets, mainly due to its nutritional properties and health benefits. Bee pollen is the result of the agglutination of flower pollens; it is made by worker honey bees with nectar and salivary substances and stored at the hive entrance [1]. The collection of bee pollen is a relatively recent development, dependent primarily on the basic concept of scraping pollen off of the bees’ legs as they enter the hive. When analyzing and studying the nutritional and therapeutic properties of bee pollen, modern science has made it possible to specify its valuable antimicrobial [2], antifungal [3], antioxidant [4], anti-radiation [5], hepatoprotective [6], chemopreventive [7], anticancer [8] and antiinflammatory activities [9]. 190

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2014 Vol. 8 No. 3

ISSN 2170‐1768 

Composition chimique de l’huile essentielle du Fenouil ( Foeniculum vulgare) de la région de Mascara OUIS N.1, HARIRI A.2 & EL ABED D.1 1

Laboratoire de Chimie Fine, Département de Chimie, Faculté des SciencesExactes et Appliquées, Universitéd’Oran, B.P.-1524 - Menouar, 31 000 – Oran, Agérie 2 LaboratoireBioconversion, géniemicrobiologique et sécuritésanitaire, Faculté des Sciences de la Nature et de la vie, Université de Mascara, BP. 763, Sidi Said, Mascara, 2900, Algérie

Received: December 15, 2013; Accepted: March 23, 2014 Corresponding author Email [email protected]

Copyright © 2014-POSL DOI:10.163.pcbsj/2014.8.3.198  

Résumé. Le Fenouil (Foeniculum vulgare) est une plante aromatique appartenant à la famille des Apiaceae. Elle est traditionnellement utilisée dans le traitement des troubles digestifs.L'huile essentielle des graines de fenouil (Foeniculumvulgare)extraite par hydrodistillationa été caractérisée par ses propriétés physico-chimiques et analysée par chromatographie GC et GC / MS. Les résultats de l’analyse ont révélé que l’estragole constitue le produit majoritaire de l’essence (83,10%). Le limonène et la fenchoneen sont les principaux monoterpènes. Mots clés : Foeniculum vulgare, Huile essentielle, Hydrodistillation, Analyse, CPG

Chemical composition of the essential oils of Foeniculum vulgare from Mascara district Abstract. Fennel (Foeniculum vulgare) is an aromatic plant belonging to the family Apiaceae. It is

traditionally used in the treatment of digestive disorders. The essential oil of fennel extracted by steam distillation was characterized by its physicochemical properties and analyzed by chromatography GC and GC/MS. The results of analysis revealed that the major product of essential oil is estragol(83,10%).Limonene and fenchone are the main monoterpenes.

Key Words: Foeniculum vulgare, Essential oils, Hydrodistillation, Analysis, GC

1. Introduction Le fenouil ( Foeniculum vulgare) originaire de la méditerranée est considéré comme une plante aromatique et médicinale. Elle est utilisée comme aromates dans les préparations culinaires, servant principalement comme correcteur de goût dans l’industrie agro-alimentaire (confiseries, pains,…). En médecine traditionnelle, le fenouil est très usité pour ses effets carminatifs, sédatifs, apéritifs, stomachiques, antispasmodiques, etc… [1]. Afin de valoriser la qualité de l’huile essentielle extraite à partir des graines de fenouil de Mascara (Nord ouest algérien), nous avons déterminé ses propriétés physico-chimiques et 198   

PhytoChem & BioSub Journal Peer-reviewed research journal on Phytochemistry & Bioactives Substances

ISSN 2170 - 1768

ISSN 2170-1768

POSL

Edition LPSO Phytochemistry & Organic Synthesis Laboratory http://www.pcbsj.webs.com , Email: [email protected]

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