University of Sulaimani Faculty of Agricultural Sciences Field Crops Department
Course Syllabus
Advanced Molecular Genetic Marker Techniques Spring 2014-2015 Second Semester
Instructor: Dr. Nariman Salih Ahmad E-mail address:
[email protected] Office: Telephone:
Crop Sciences department, Ground floor, Office no. 10 (+964) 770 143 487 3, (+964) 750 106 603 8
Education cycle: Postgraduate students (M Sc) Graduate credit: Three (3 hrs lecture in a week).
Advance Molecular Genetic Marker Techniques
MSc course 2014-2015
Learning outcomes: By the end of this advanced course the students will be able to:
1. Understand and explain scientific principles behind genetic, molecular markers and genome mapping. 2. Analyse information from plant molecular techniques and recognize their potential applications in crop improvement. 3. Evaluate the relative merits of plant transformation, marker-assisted breeding and conventional phenotypic selection for particular situations. 4. Synthesize information from plant molecular biology and plant breeding to design plant molecular breeding strategies. 5. Demonstrate learning processes skills in collaborative group, developing technologies and hypothesis and the ability to apply these principles to a specified project.
Course Description: This course involves teaching sessions for Postgraduate students. Plant molecular techniques will be demonstrated in the way can be incorporated into crop improvement programs via manipulation the gene technology and the application of genetic marker information. This course considers the scientific techniques for the application of genetic diversity study, gene flow, validation the segregation population, Linkage map construction and tagging the specific location of gene (s) on the genetic map through QTL mapping.
Advance Molecular Genetic Marker Techniques
MSc course 2014-2015
Objectives: This course aims to explain the differences between molecular marker systems available for use in plant breeding programs. The course also aims to figure out steps necessary to implement molecular markers in a plant improving strategies. The utilizing of molecular markers extends toward the construction of genetic linkage map, QTL analysis and MAS to identify the target gene (s) for the reliable and rapid development of plant crops.
Advance Molecular Genetic Marker Techniques
MSc course 2014-2015
Lectures outlines Week
Fist
Second
Topic Molecular marker techniques: The concept and their types Classical marker Morphological marker Cytological marker Biochemical/ protein marker DNA marker Overview of different DNA markers Polymorphism of DNA markers Criteria of DNA marker
Third
Molecular Genetic Maps and Their Applications in Plant Breeding
Fourth
DNA Markers as Landmarks in Chromosomes
Fifth
The Molecular Structure of DNA Ratio of Nitrogen bases in the DNA composition
Sixth
The Separation and Identification of Genomic DNA Fragments
Seventh Eighth
Hybridization method Restriction Enzymes and Site-Specific DNA Cleavage Gel Electrophoresis Southern blotting techniques and their application in DNA markers
Ninth
Selective Replication of Genomic DNA Fragments DNA Replication: Primers and 5'-to-3' Strand Elongation
Tenth
Polymerase chain reaction Denaturation and renaturation process in fragment amplification
Eleventh
The Terminology of Genetic Analysis
Twelfth
Types of DNA Markers Present in Genomic DNA Restriction Fragment Length Polymorphisms (RFLPs) Random Amplification of Polymorphic DNA Simple sequence repeats or microsatellite marker Inter simple sequence Repeat (ISSR) marker:
Thirteen Fourteen
Amplified Fragment Length Polymorphism PCR (AFLP-PCR) Single Nucleotide Polymorphism marker Diversity array technology marker (DArT) Final exam
