Supplementary Information Figure S1. Amino acid sequence alignment of the C-terminal region downstream of the dsRBD in DGCR8/Pasha homologues from various animal species.

Table S1.

Primer sequences used for cloning and mutagenesis of human DGCR8. hDGCR8 WT

DG276-773

DG484-773

DG484-750

DG484-738

Primer sequence (5’-3’) Forward

GAATTCTGATGGAGACAGATGAGAGCCCC

Reverse

GCGGCCGCTCACACGTCCACGGTGCACAG

Forward

GAATTCACGATGGAGAGACAAGTGTGCAG

Reverse

GCGGCCGCTCACACGTCCACGGTGCACAG

Forward

GAATTCCAGCCAATCAGAAGCTCATTAC

Reverse

GCGGCCGCTCACACGTCCACGGTGCACAG

Forward

GAATTCCAGCCAATCAGAAGCTCATTAC

Reverse

GCGGCCGCTCATCGAGTCTCCTCCCTTTCC

Forward

GAATTCCAGCCAATCAGAAGCTCATTAC

Reverse

GCGGCCGCTCACTCCTCTTGGAGCTTGC

DG1-483

DG1-614

DG1-692

mDRBD1

mDRBD2

Forward

GAATTCTGATGGAGACAGATGAGAGCCCC

Reverse

GCGGCCGCTCACAAGATGGGCCTCTCGGACTC

Forward

GAATTCTGATGGAGACAGATGAGAGCCCC

Reverse

GCGGCCGCTCACTTGCTGGTCAGCTCGTAGAC

Forward

GAATTCTGATGGAGACAGATGAGAGCCCC

Reverse

GCGGCCGCTCACCCCCAGTTCTTGACATGTGG

Forward

CTTGCGAAGAATAAAAAGAAACGAGCTACACTGG

Reverse

CCAGTGTAGCTCGTTTCTTTTTATTCTTCGCAAG

Forward

GTTGGAAAGCAGTTAAAGAAACAGAAGATCCTTC

Reverse

GAAGGATCTTCTGTTTCTTTAACTGCTTTCCAAC

Supplementary Information

Page 1. Supplementary Information. Figure S1. Amino acid sequence alignment of the C-terminal region downstream of the dsRBD in. DGCR8/Pasha ...

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