Set No:

Code No:RR-222305 II-B.Tech. II-Semester Regular Examinations, April/May-2004

1

MOLECULAR BIOLOGY (BioTechnology) Max. Marks: 80 Answer any FIVE questions All questions carry equal marks ---

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Time: 3 Hours

What are the features of the DNA molecule described by Watson and Crick? Are these features same for all DNA molecules?

2.

What do the following enzymes do in DNA replication: DNA polymerase 5’ to 3’ exonuclease activity, initiator protein, helicase, topoisomerase.

3.

Write the differences between the eukaryotic and prokaryotic RNA polymerases and their mechanism.

4.

Indicate the role of cysteine in stabilizing the tertiary structure of secretory proteins.

5.

Briefly describe physical, chemical and biological methods of developing new mutations.

6.

What do you mean by complementation and recombination process?

7.

Distinguish among the three modes of recombination in bacteria.

8.

What is the benefit of signal cascades that are often found in signal transduction pathways?

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Set No:

Code No:RR-222305 II-B.Tech. II-Semester Regular Examinations, April/May-2004

2

MOLECULAR BIOLOGY (Bio-Medical Engineering) Max. Marks: 80 Answer any FIVE questions All questions carry equal marks ---

.in

Time: 3 Hours

How do other DNA molecules differ from those described by Watson and Crick?

2.

DNA replication is bi-directional. What would the replication bubble look like (using the same labeling regimen as discussed in class) if replication was unidirectional?

3.

Where are each of the RNA polymerase types found in the eukaryotic cell?

4.

What will determine whether regions of alpha-helical structure lie at the surface or in the interior of a water-soluble globular protein?

5.

Describe different types of mutations; and explain the importance of mutations for genetic research.

6.

Write a note on molecular structure of eukaryotic gene.

7.

Describe the basis for chromosome mapping in the Hfr x F- crosses.

8.

What is a ligand? What is the property of ligands that accounts for the two major classifications of signal transduction.

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Set No:

Code No:RR-222305 II-B.Tech. II-Semester Regular Examinations, April/May-2004

3

MOLECULAR BIOLOGY (Bio-Medical Engineering) Max. Marks: 80 Answer any FIVE questions All questions carry equal marks ---

.in

Time: 3 Hours

What are the roles of RNA primers and Okazaki fragments during DNA replication?

2.

Why is the 3’OH group on the ribose ring so important for DNA and RNA synthesis?

3.

How does the structure of a eukaryotic CoreRNAPol compare with the E. coli CoreRNApol?

4.

Why would aspartate be more likely to occur in a beta turn than valine? What other amino acids would occur more often in a beta turn than valine?

5.

Most mutations are thought to be deleterious. Why then, is it reasonable to state that mutations are essential for evolutionary process?

6.

Write a note on intervening sequences and split genes.

7.

Why are the recombinants produced from an Hfr X F- cross never F+?

8.

What are the two major classifications of signal transduction (due to the

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two major places receptors are found)?

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Set No:

Code No:RR-222305 II-B.Tech. II-Semester Regular Examinations, April/May-2004

4

MOLECULAR BIOLOGY (Bio-Medical Engineering) Max. Marks: 80 Answer any FIVE questions All questions carry equal marks ---

.in

Time: 3 Hours

What is the relationship between nucleosomes, 30 nm fibers and the scaffold structure with respect to the organization of DNA in the nucleus?

2.

What are the two differences between DNA and RNA? What bases pair with each other?

3.

What is the nature of the eukaryotic and prokaryotic Promoter?

4.

Why would isoleucine be less likely to occur in a beta turn than in a beta strand?

5.

Describe tautomerism and the way in which theis chemical event may lead to mutation.

6.

Write the fine structure and function of a gene.

7.

Describe the origin of F’ bacteria and merozygotes.

8.

What are the difficulties in transducing a signal outside the cell into a cellular response inside the cell?

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exonuclease activity, initiator protein, helicase, topoisomerase. 3. Write the differences between the eukaryotic and prokaryotic RNA polymerases · and their ...

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