Workshop on Biomaterials and Their Interactions with Biological and Model Membranes Altafulla 2017

Reversible Adhesion of Particles on Lipid Bilayers through Electrostatics or Depletion Hendrik Th. Spanke, Claire François-Martin, Eric R. Dufresne [email protected] [email protected] Department of Materials (ETH Zürich, Vladimir-Prelog-Weg 5, CH-8093 Zürich)

Abstract Biological membranes partition living organisms and eukaryotic cells into different compartments, each of which having its own function and integrity. Moreover, some organelles, such as the endoplasmic reticulum, are characterized by very complex membrane shapes, which seem to confer to them, at least partly, their function. The regulation of biological membrane geometries is therefore crucial. In vivo, proteins are one of the main actors of membrane deformation, as they are able to sense curvature and bend membranes. While many different curvature stabilizing proteins have been identified, the physical mechanisms underlying their function are not well understood. On the other hand, 3D membrane geometries have been observed in protein-free systems as the result of the adsorption of particles on membranes [1,2]. Thus, the adsorption and self-assembly of simple colloidal particles could serve as a useful model system to reveal the essential physics of membrane deformation by bound proteins. Several theoretical and numerical studies have predicted how particles behave when adsorbed to membranes [3,4,5]. However, relevant experimental data is lacking, mainly due to the fact that these studies typically use irreversible binding of particles to membranes [6,7]. In this work, we present two approaches for reversible binding of particles to membranes: depletion and electrostatic interactions. In the case of depletion we add 1 µm Polystyrene-particles to lipid membranes in the presence of Polyethylene glycol (PEG). Strength and range of the depletion interactions can be finely controlled by the amount and molecular weight of the depletant polymer chains. In the case of electrostatics we add polystyrene-particles coated with PEG-poly-L-lysine (PEG-PLL) to negatively charged lipid bilayers. PLL is positively charged and allows the particle to adsorb on the membrane while PEG adds a steric hindrance preventing it from adhering too strongly.

References: [1] [2] [3] [4] [5] [6] [7]

Dietrich et al., J Phys II 7, 1651 (1997) Yu and Granick, JACS 131, 14158 (2009) Xiong et al., Soft Matter 13, 4644 (2017) Deserno, PRE 69, 031903 (2004) Bahrami et al., Advances in Colloid and Interface Science 208, 214 (2014) Sarfati and Dufresne, PRE 94, 012604 (2016) van der Wel et al., Scientific Reports 6, 32825 (2016)

Reversible Adhesion of Particles on Lipid Bilayers ...

Biological membranes partition living organisms and eukaryotic cells into different compartments, each of which having its own function and integrity. Moreover, some organelles, such as the endoplasmic reticulum, are characterized by very complex membrane shapes, which seem to confer to them, at least partly, their ...

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