Open OLGA Bioreactor by Alexander Murer Version 1.0: The idea was to create a modular bioreactor based on off the shelf parts for biohackerspace use and beyond. I spent quite some time on planning how to built the OLGA Bioreactor as simple and robust as possible. All modules are standalone, so they can be updated, automated, removed or easily repaired. The volume is 1 liters and can be upgradet to 2 liters when you use a bigger acrylic mantle + 2 liters duran vessel. All media touching parts can be autoclaved. Thanks to Kilobaser for the support.
Temperature module: The OLGA Bioreactor uses a double mantle filled with water to heat the inner vessel. The inner vessel rests on 3 silicon rubber pieces, while a magnetic stirrer in the double mantle ensures even temperature. The hotplate comes with an attached temperature probe, which is inserted into the double mantle through a small hole on top. The bottom consists of 2 aluminium plates. One main plate and a much smaller one which has direct contact with the heat plate, to prevent the very hot heatplate from melting the acrylic.
The cycle peristaltic pump constantly pumps media from the main vessel through the flow cell with the inserted pH probe. The smaller dosing pump, conntected to a vessel with acid or base starts pumping when the controller measures a certain pH and when the timer is on an active cycle. The timer is used so that the dosing pump only pumps a certain time and then waits before it continues (for example: 1 second pumping then 10 second waiting). Mixing and pumping new media from the main vessel into the flow cell takes a while, so we need some delay on that. The device has 3 switches: 1.) general on/off 2.) pumps on/off, so they don’t operate before you set a pH value on the controller 3.) fill tube, which actives the acid/base pump so you can fill the tube to avoid slow dosing when the system was just started.
Air module: A small air pump presses air through a sterile filter into the main vessel via a ptfe tube to the buttom of the vessel. Flowmeter: https://de.aliexpress.com/item/High-quality-0-1-1-5L-oxygen-air-flow-meter-gas-flowmeterwith-copper-connector/32290568531.html?spm=a2g0s.9042311.0.0.J0LCYZ Air pump (5€) https://de.aliexpress.com/item/New-DC-12V-Micro-Air-Pump-Electric-Pumps-Mini-VacuumPump-Pumping-Booster-For-Medical-Treatment/32797393859.htm Filter, PTFE in-line (30€) https://shop.bartelt.at/info562_LLG-InlineFilter_lang_DE.htm?UID=550059bc6574000000000000
Speciality: Applied mathematics and computer science. Master Degree ... 1. HISTORY OF THE PROBLEM. Considering the history of the problem, it is necessary to say first about a ... quantities of higher degrees are lengthy and cannot be obtained ... Th
Phone: +7 921 4023104. Address: P.O. Box 35 (Agora), FI-40014 University .... algorithms for computation software packages. (MatLab in my case) are created.
We have developed a two-phase flow Finite Volume model, considering degradation and heat production. Mass Conservation. Energy Conservation. ( ) 0 . = â+.
Aug 6, 2017 - 2009 David Gifford Laboratory, MIT Computer Science and Artificial .... Taught three weeks of git, RNA-seq and analysis methods to graduate-level UCSD course ... Developed interactive curriculum for online Bioinformatics ...
Web Site: http://www.commack-umc.org ..... and drizzle to host a campfire where they had something most homeless ... One of our best selling items is Jewelry.
will present the program "How Are We. Raced", to ... now home and will be going to Florida for a couple of ... Island Ducks Game, another good time as usual.
Web Site: http://www.commack-umc.org ... may be difficult, even confusing in your own particular life. ... design or drawing that is a visual reminder of the time.
Oct 5, 2008 - Emails: [email protected][email protected]. CELEBRATING 225 YEARS OF CHRISTIAN SERVICE IN COMMACK. 1783-2008. Rev. ..... Please send cards & good wishes to Phyllis at her new address: c/o ...
Nov 9, 2006 - Pastor's E-Mail: [email protected]. Rev. Richard C. ... memorial service for Judi in a church in. Columbia ... The list is long of all the things they gave;. Our veterans ... Our veteransâthe very best on earth. By Joanna ...
Email: [email protected]. Pastor's E-Mail: [email protected]. Rev. Richard C. Mills, Pastor (631) 499-4770. November 2005. Open Hearts Open Minds Open Doors .... there are only nine Trustees and it would be impossible for us to do our job wit
Jun 16, 2007 - Pastor's E-Mail: [email protected]. Rev. Richard C. Mills, Pastor (631) 499-4770. June 2007. Open Hearts .... we will do our best to respond. We wish you all a happy and blessed summer. Anne Tammaro & Gail ... The UMW hosted a cof
all, I would like to express my deepest gratitude to ... Thank you all again for all your love and support! .... relish dish and assorted deserts, coffee tea and apple.
At 6:00pm, the 14 members of BSA. Troop 125 arrived, looking great in their uniforms. Joan and Tricia Nehlsen,. Harriet Neuberth, Karen Mallgraf, Tracy.
Emails: [email protected] ... CELEBRATING 225 YEARS OF CHRISTIAN SERVICE IN COMMACK. 1783-2008 ... God is good and it feels like a good gift from God to ... their birth months for this greetings list. ... Everything will be free.
Training; Brazil. Youth Training and Human Rights;. Brazil ... the Confirmation Service on Sunday,. June 4 th ... puppets), Craft Helpers and Games. Leaders as ...
Oct 5, 2008 - career in the service of the Lord. I recall, gratefully, the warm welcome extended to the bride I brought with me in 1944, with whom I have spent ...
May 20, 2006 - Northport, a Northport Opera Company soprano sang three liturgical .... Apple Bank for Savings in Commack and assistant manager Nancy ...
Pastor's E-Mail: [email protected]. Rev. ... how many good works are left undone because they were never started. God has called you to his service, it is .... that free item for our food box. .... Everyone needs this list to live byâ¦pass.
Dec 9, 2007 - Pastor's E-Mail: [email protected]. Rev. .... You are invited to add your old or favorite family .... http://www.iGive.com/html/refer.cfm?m.
Jan 1, 2006 - The theme for 2006 is âPreserving. Property; Serving Peopleâ and the ... participate! The wearing of your heritage/native dress will add to the.
it a priority, it all too often falls behind when my schedule is uneven or ... I claimed that a church where folks, all ... Lynda, we will share fellowship in an APPLE.