Shree Manibhai Virani and Smt. Navalben Virani Science College, Rajkot (Autonomous) Affiliated to Saurashtra University, Rajkot Department of Biotechnology M.Sc.(Biotechnology) Entrance Syllabus, 2016 Unit-1.General Biology Taxonomy, Prokaryotic and eukaryotic organisms, Internal transport systems

of

plants,

Reproduction

in

plant

system,

Transpiration,

phytochromes & cryptochromes, Phytohormone, Regulations of body fluids and

excretory

mechanisms,

Nervous

system,

Endocrine

system,

Cardiovascular system, Digestive system, Reproductive System.

Unit-2.Cell & Molecular biology Organization of cell, plasma membrane, transport across membrane, Cell organelles & its Function, Cell-cell interaction, cell division, cell cycle & regulation, Nature of gene, replication, repair, Transcription ,Translation, Recombination, Gene regulation in prokaryotes and eukaryotes, apoptosis, cell differentiation, cancer biology, cell signalling, method in molecular biology.

Unit -3.Bio-molecules & Energetics Structure of atoms, molecules and chemical bonds ,General property of water, Thermodynamics and bioenergetics ,Classification, structure and function of Carbohydrate, lipid and protein, Metabolism of Carbohydrates ,lipid & amino acid, nitrogen fixation, Photosynthesis, Sequencing of DNA & protein , Nucleotides & its metabolism, vitamin, Enzymes: mechanism of action, Inhibition, kinetics and regulation, Biochemical techniques. 1

Unit -4.Microbiology & Immunology Evolutionary overview of microbes, general character & classification of algae, fungi & bacteria, Ultra structure , nutrition, control and growth, Cultivation and preservation techniques, antibiotics & mode of action, Microscopy and staining techniques, bacterial genetics, archaebacteria, virus, type of immunity, cell & organ of immune system, Antigen and Antibody: structure and interaction , immunediversity , MHC, compliment system, cytokine, hypersensitivity,, vaccine, autoimmunity, HIV & other immunodeficiency, prions, immunotechniques.

Unit-5.Genetics Mendelian principles, deviation from Mendelian inheritance, penetrance and expressivity, linkage and crossing over, sex linkage, sex limited and sex influenced characters, Linkage maps, Linkage and genetic mapping, Sex determination, extra chromosomal inheritance, Mutation and chromosome aberration, Genetic disease: diagnosis & therapy Genome organization, population genetics, Epigenetics and Eugenics.

Unit-6. Ecology & Environmental Biotechnology Biotic and abiotic interactions,Concept of habitat and niche,Population ecology,Types of interact,community structure and attributes,levels of species diversity and its measurement; edges and ecotones,Succession, changes involved in succession,concept of climax,Ecosystem: Structure and function; energy flow and mineral cycling (CNP); primary production and decomposition; Bioremediation, Bioleaching, Bioplastic, Biocontrol, Biofuel.

Unit-7.Instrumentation & Analytical techniques

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Centrifugation, Spectroscopy, Chromatograph, Electrophoresis, Dialysis, structure determination using X-ray diffraction and NMR, Biosensor, Electrophoretic Techniques, fluorescence, circular dichroism, Radiolabelling technique,

Unit-8.Genetic engineering & Bioinformatics Basic principle of gene cloning, Steps of gene cloning, Vector, Restriction endonuclease, PCR, blotting techniques, Screening of Recombinants, DNA modifying enzyme, Recombinant protein production, study of gene location and expression, Application of gene cloning in research, medicine and agriculture, Nucleic acid and protein sequence databases, Proteomics, Genomics: structural and functional genomics, Gene prediction and comparative genomics, Drug designing, Microarray technology, Databases.

Unit-9.Applied Biotechnology Microbial fermentation and production of small Screening of Recombinants & Downstream processing, Vaccine production, Basics of cell culture methods for plants and animals, Method of DNA delivery, transgenic animals and plants, molecular approaches to disease diagnosis.

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