St. Joseph’s College of Arts & Science (Autonomous) St. Joseph’s College Road, Cuddalore – 607001
PMB703 – MOLECULAR BIOLOGY AND MICROBIAL GENETICS Time : 3 hrs Max Marks :75
SECTION – A (5X5=25) Answer any FIVE Questions 1. Write about the secondary structure of RNA molecule. 2. What are the properties that makes DNA as repository of information? 3. State the three models of DNA replication. 4. Write note on biodirectional replication. 5. Defineauxotrophic mutants, how it can be created? 6. With neat sketches narrate the first observation of transformation phenomena. 7. Give the features of the M13 bacteriophage. 8. Write short notes on single step growth curve of bacteriophages. SECTION - B (5X10=50) Answer any FIVE Questions 9. Write in detail about three dimensional structure of the DNA molecule.
1
L1P1/20N15
10. Explain Sanger’s method of determining bases sequences of the DNA molecule. 11. State and explain the enzymes and proteins involved in the DNA replication. 12. Describe semi discontinuous replication of DNA molecule with a neat diagram. 13. Explain the steps in the prokaryotic gene transcription. 14. How competent cells are prepared for transformation? Explain the rationale
behind
the
recombinant
selection
by
insertional
inactivation of antibiotic genes. State the applications of transformation. 15. What are the steps in phage infection cycle? Explain gene organization in DNA. 16. What is transfection? How the recombinant phage particles are packed into the E. coli cell? ************
MOLECULAR BIOLOGY AND MICROBIAL GENETICS - 11 15.pdf ...
Write about the secondary structure of RNA molecule. 2. What are the properties that makes DNA as repository of. information? 3. State the three models of DNA ...
What is axoneme? 14. What is Laminin? 15. Define oncogenes. SECTION B (5x4=20). Answer ALL the questions. 16. a) Explain the structure of nucleus. (Or).
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... Molecular Biology, Evolution and Ecology by Verma, Agarwal.pdf. Cell Biology, Genetics, Molecular Biology, Evolution and Ecology by Verma, Agarwal.pdf. Open. Extract. Open with. Sign In. Main menu. Displaying Cell Biology, Genetics, Molecular Bio
Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 03755. INTRODUCTION . ...... transport (appC), DNA replication-repair (mutT), and compe- tence (comD). ..... Conjugal transmission of plasmids. Annu. Rev. ..... Bacterial gr
Michael A. Lieberman, Ph.D. Dean, Instructional and Research Computing, UCit. Distinguished Teaching Professor. University of Cincinnati. Cincinnati, OH. Page 3 of 398. BRS Biochemistry, Molecular Biology,and Genetics 5th edition.pdf. BRS Biochemistr
Page 3 of 398. Biochemistry, Molecular. Biology, and Genetics. Todd A. Swanson, M.D., Ph.D. Resident in Radiation Oncology. William Beaumont Hospital. Royal Oak, Michigan. Sandra I. Kim, M.D., Ph.D. Division of Nuclear Medicine and Molecular Imaging.
closure (syndromes of craniosynostosis) has uncovered the first clues to some key molecules and their specific roles in this process. The list, which is expected ...
