Conservation Genet Resour (2015) 7:917–944 DOI 10.1007/s12686-015-0493-8
MICROSATELLITE RECORDS
Microsatellite records for volume 7, issue 4
Springer Science+Business Media Dordrecht 2015
Electronic supplementary material The online version of this article (doi:10.1007/s12686-015-0493-8) contains supplementary material, which is available to authorized users.
123
123
Osmoderma barnabita
Hermit_07*,b
Hermit_06b
Hermit_05b
Hermit_02*,b
Hermit_01b
HT029a
HT028a
HT027a
HT025a
HT023a
HT018a
HT016a
HT015a
HT012a
HT009a
HT007a
HT005a
HT002a
F: CTGGACATTGCTGAGGAA
HT001a
Hemifusus tuba (Gmelin)
R: CAATGAGATAGCGGTGATGAT
F: GTTAACCGATCGGAAATGAAT
R: TGGCAATCGGGCAAGTT
F: CACACCTCTAAAGTAATCGTT
R: GTATCACTCTACATTAGGTTTGTGT
F: GAACGCGAAGGTCCAATAAAT
R: TTGCGACATAATAAAACAGG
F: GGCTGCTCCGAAGACA
R: GTTCCTGGACGGGTTGT
F: ATTGCACGATTACTATG
R: GAGAAAGTGAATGAGAGTGGGCG
F: TTGACTGGGGTGTGGGGTT
R: TCGCAAAAAGTGAAGGGCGGTAG
F: TGGTCACACTAGACTGACGACGCAT
R: AGCCCAATACCCAAACCCCACTTAC
F: TGGTCACACTAGACTGACGACGCAT
R: CCGATTGACCAGAGACCCCA
F: GAGAGCGACGAACGGAAGGA
R: GTAGGGGCTGTCAGAGATAA
F: TGTACCACTTGAACTGGTCC
R: TTGGTAATAGGGGCTTCC
F: CTCCCTTGATTTCACCTTC
R: GCCAGGGTTTTCCCAGTCA
F: CGTATTCGGCGGCAATCA
R: CTCAATCTCCAAGCAGAGTTGTCG
F: CGTGCATGTCGTGATAAGTCGG
R: GGGGAAAACCTAACCACT
F: CAACTTCTGTAGCCTGTAATG
R: TTCTCGGACTTCGGTTTCTT
F: GGGTACATAAGGCTCGTTCA
R: TGTCTTCTTACTGCCATTTC
F: TTCCTGTATTTCTGCCTCT
R: TTCACCGTCACATCAACTTCCCT
F: CCCAGCACAACAAACGACAGA
R: AATGAGACGACGGCAGACTTTG
F: ATTGGACTTCTTCGGCATAGCG
R: GCGGGTATGAATCGAGAT
Primer sequence (50 –30 )
Locus
Species
(AC)12
(AC)14
(AC)14
(AC)11
(AC)16
(TGTC)4(TC)5(CT)23(CT)18
(GA)39
(CT)32(TG)19
(AG)38
(TCC)7(CT)4
(GT)19(TG)16(TC)7(TC)9(TC)9(TC)16
(GA)6(AG)8(AG)29
(TC)12(CT)17(TC)28
(CT)5(TC)20(TC)29
(AG)62(AG)4 (AG)7(GA)4(GA)8(GA)14(AG)7
(AG)43(TG)4(TG)4
(GT)19(TG)16(TG)10(TC)7(TC)9(TC)9(TC)16
(GA)32
(CAAT)8(TC)27
Repeat motif
385–387
164–178
291–315
264–266
217–223
172–184
244–258
198–244
134–156
172–180
108–130
284–300
288–308
246–260
194–220
224–240
144–260
345–400
342–360
Size range (bp)
45
45
45
45
45
58
54
52
56
56
54
52
56
62
58
54
58
62
60
Sample size (N)
2
4
4
2
2
3
3
12
7
2
7
5
7
2
6
4
8
3
2
Number of alleles (NA)
0 (0.044)
0.244 (0.288)
0.111 (0.108)
0.889 (0.501)
0.111 (0.145)
0.1333 (0.3774)
0.1429 (0.1377)
0.6667 (0.8784)
0.6207 (0.8149)
0.0714 (0.0701)
0.8214 (0.8169)
1.0000 (0.6101)
0.4483 (0.6999)
0.0667 (0.1831)
0.3571 (0.5552)
0.1852 (0.5304)
0.7500 (0.7883)
0.2333 (0.2164)
0.0667 (0.0655)
Ho (He)
918 Conservation Genet Resour (2015) 7:917–944
Osmoderma eremita
Species
Hermit_12*,#,b
Hermit_11b
Hermit_10b
Hermit_09b
Hermit_08b
Hermit_07*,b
Hermit_06b
Hermit_05b
Hermit_02*,b
Hermit_01b
Hermit_16b
Hermit_15b
Hermit_14b
Hermit_13b
Hermit_12*,#,b
Hermit_11b
Hermit_10b
Hermit_09b
F: AGTTCTTCGTTGAGGGATAAT
Hermit_08b
R: GCGGCGCATTCATCAA
F: GAATCACGGGTTAATTACCTACTAA
R: TATTTAGGCAGCCAAGGAGGTG
F: GAGTGAAGTAATACGCAAGTGAGAG
R: AGACCTCGAACTTACAG
F: CATTGCCTTGCTACA
R: GATTTAGATTCGCTTGTA
F: AATCATTTTCGGAGGTG
R: GTGAAAAGCTGTAAACTGGATA
F: AGTTCTTCGTTGAGGGATAAT
R: CAATGAGATAGCGGTGATGAT
F: GTTAACCGATCGGAAATGAAT
R: TGGCAATCGGGCAAGTT
F: CACACCTCTAAAGTAATCGTT
R: GTATCACTCTACATTAGGTTTGTGT
F: GAACGCGAAGGTCCAATAAAT
R: TTGCGACATAATAAAACAGG
F: GGCTGCTCCGAAGACA
R: GTTCCTGGACGGGTTGT
F: ATTGCACGATTACTATG
R: AGGTGCCAGAGGTACATTTGAA
F: GGAACGCGCAGATAGGACT
R: TACCGGAGCCGTTGGAGAT
F: CACGCCATGGATGTTGAAAA
R: TGGGCAGTCAGTAATAACC
F: AGGAAATCGTGCATAGTCAATA
R: CGTCATGAGTTGGTGGTTT
F: CAAGTTGGTCAGTAATAGGAGAA
R: GCGGCGCATTCATCAA
F: GAATCACGGGTTAATTACCTACTAA
R: TATTTAGGCAGCCAAGGAGGTG
F: GAGTGAAGTAATACGCAAGTGAGAG
R: AGACCTCGAACTTACAG
F: CATTGCCTTGCTACA
R: GATTTAGATTCGCTTGTA
F: AATCATTTTCGGAGGTG
R: GTGAAAAGCTGTAAACTGGATA
Primer sequence (50 –30 )
Locus
(AG)18
(AG)19
(AG)18
(AC)10(AG)7
(AC)12
(AC)12
(AC)14
(AC)14
(AC)11
(AC)16
(AT)11
(AG)15
(AG)12
(AG)4 GG(AG)11
(AG)18
(AG)19
(AG)18
(AC)10(AG)7
(AC)12
Repeat motif
–
189
267–269
378
–
–
164
–
260
–
370–372
449–453
380–390
219–235
310–322
189–231
249–261
353–357
248
Size range (bp)
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
45
Sample size (N)
1
2
1
1
1
2
3
4
3
5
13
5
3
1
Number of alleles (NA)
–
–
0.067 (0.065)
–
–
–
–
–
–
–
0.044 (0.044)
0.089 (0.087)
0.089 (0.087)
0.044 (0.044)
0.244 (0.626)
0.778 (0.868)
0.289 (0.336)
0.067 (0.108)
–
Ho (He)
Conservation Genet Resour (2015) 7:917–944 919
123
123
Opsaridium microcephalum (Bua River)
Opsaridium microlepis (N.Rukuru River)
Species
Ops7c
Ops5c
Ops4c
Ops3c
Ops22c
Ops20c
Ops19c
Ops16c
Ops13c
Ops11c
Ops9c
Ops7c
Ops5c
Ops4c
Ops3c
Hermit_16b
Hermit_15b
Hermit_14b
F: CAAGTTGGTCAGTAATAGGAGAA
Hermit_13b
R: AGACACTGCTGAAGGACCG
F: TCATTTCTCTTGGGATTGTTTGG
R: CAAGTTAAGGCTGCTCTGGG
F: AATGTGTGCAGCGCTCAAG
R: GGTTTGGACAACAACCATGC
F: TCAGCAATGCATCACCCTG
R: GGTTCGAAACGATATGAGGGTC
F: ATGTTGCATGAAGCACCTG
R: AGGAATAACACAAATTATGGGACAG
F: TCCGGGCCACTGAAATAGAC
R: GCATGGATTCCTGCCACAAA
F: CACAGAATCAGGGATGCAGC
R: TGTGCCCTGATAAAAGCTGC
F: CATTCACACCGTTCCTCTGC
R: CCGCTCCAATCTGGATTCAAC
F: GAAATGGAGTGTCATTTAGCATTAC
R: CGCTGAAACCGAGAGGTTG
F: ATTTCCACGCGCTGAACTC
R: AGCAGCTCGACCAAACTAGT
F: GGGCTGAATGACGCTCTTAA
R: AGGGATCAAGAGTGTGCCTT
F: AGCATAGTGGACAGCTCAGT
R: AGACACTGCTGAAGGACCG
F: TCATTTCTCTTGGGATTGTTTGG
R: CAAGTTAAGGCTGCTCTGGG
F: AATGTGTGCAGCGCTCAAG
R: GGTTTGGACAACAACCATGC
F: TCAGCAATGCATCACCCTG
R: GGTTCGAAACGATATGAGGGTC
F: ATGTTGCATGAAGCACCTG
R: AGGTGCCAGAGGTACATTTGAA
F: GGAACGCGCAGATAGGACT
R: TACCGGAGCCGTTGGAGAT
F: CACGCCATGGATGTTGAAAA
R: TGGGCAGTCAGTAATAACC
F: AGGAAATCGTGCATAGTCAATA
R: CGTCATGAGTTGGTGGTTT
Primer sequence (50 –30 )
Locus
(ATT)11
(GAT)12
(ATT)12
(AAC)11
(GATT)8
(ATCT)14
(ATCT)11
(AGAT)11
(CAAA)5
(ATC)21
(ATT)13
(ATT)11
(GAT)12
(ATT)12
(AAC)11
(AT)11
(AG)15
(AG)12
(AG)4 GG(AG)11
Repeat motif
377
424
270–281
402–430
384–410
205
241–277
313–373
362
249–285
270–287
385–400
413–445
273–303
396–430
372
–
–
229–231
Size range (bp)
46
55
49
53
46
47
44
43
43
44
47
45
43
46
43
45
45
45
45
Sample size (N)
1
1
2
5
4
1
9
10
1
11
3
4
4
6
5
1
2
Number of alleles (NA)
–
–
0.18 (0.17)
0.36 (0.53)
0.54 (0.50)
–
0.80 (0.80)
0.49 (0.83)
–
0.73 (0.76)
0.04 (0.04)
0.42 (0.43)
0.53 (0.56)
0.65 (0.63)
0.56 (0.72)
–
–
–
0.133 (0.164)
Ho (He)
920 Conservation Genet Resour (2015) 7:917–944
Opsaridium tweddleorum (Ruhuhu River)
Species
Ops22c
Ops20c
Ops19c
Ops16c
Ops13c
Ops11c
Ops9c
Ops7c
Ops5c
Ops4c
Ops3c
Ops22c
Ops20c
Ops19c
Ops16c
Ops13c
Ops11c
F: AGCATAGTGGACAGCTCAGT
Ops9c
R: AGGAATAACACAAATTATGGGACAG
F: TCCGGGCCACTGAAATAGAC
R: GCATGGATTCCTGCCACAAA
F: CACAGAATCAGGGATGCAGC
R: TGTGCCCTGATAAAAGCTGC
F: CATTCACACCGTTCCTCTGC
R: CCGCTCCAATCTGGATTCAAC
F: GAAATGGAGTGTCATTTAGCATTAC
R: CGCTGAAACCGAGAGGTTG
F: ATTTCCACGCGCTGAACTC
R: AGCAGCTCGACCAAACTAGT
F: GGGCTGAATGACGCTCTTAA
R: AGGGATCAAGAGTGTGCCTT
F: AGCATAGTGGACAGCTCAGT
R: AGACACTGCTGAAGGACCG
F: TCATTTCTCTTGGGATTGTTTGG
R: CAAGTTAAGGCTGCTCTGGG
F: AATGTGTGCAGCGCTCAAG
R: GGTTTGGACAACAACCATGC
F: TCAGCAATGCATCACCCTG
R: GGTTCGAAACGATATGAGGGTC
F: ATGTTGCATGAAGCACCTG
R: AGGAATAACACAAATTATGGGACAG
F: TCCGGGCCACTGAAATAGAC
R: GCATGGATTCCTGCCACAAA
F: CACAGAATCAGGGATGCAGC
R: TGTGCCCTGATAAAAGCTGC
F: CATTCACACCGTTCCTCTGC
R: CCGCTCCAATCTGGATTCAAC
F: GAAATGGAGTGTCATTTAGCATTAC
R: CGCTGAAACCGAGAGGTTG
F: ATTTCCACGCGCTGAACTC
R: AGCAGCTCGACCAAACTAGT
F: GGGCTGAATGACGCTCTTAA
R: AGGGATCAAGAGTGTGCCTT
Primer sequence (50 –30 )
Locus
(GATT)8
(ATCT)14
(ATCT)11
(AGAT)11
(CAAA)5
(ATC)21
(ATT)13
(ATT)11
(GAT)12
(ATT)12
(AAC)11
(GATT)8
(ATCT)14
(ATCT)11
(AGAT)11
(CAAA)5
(ATC)21
(ATT)13
Repeat motif
418–434
181–209
249–281
353–373
183–354
249–253
267–273
372–375
–
279–282
403
409–421
190–209
249–326
322–385
352–362
247–251
252–262
Size range (bp)
18
18
18
17
18
18
18
18
–
18
18
45
51
56
51
56
56
51
Sample size (N)
3
3
7
5
2
2
2
2
–
2
1
2
2
6
11
2
2
4
Number of alleles (NA)
0.44 (0.45)
0.33 (0.37)
0.44 (0.72)
0.18 (0.44)
0.06 (0.06)
0.17 (0.25)
0.06 (0.06)
0.33 (0.36)
–
0.33 (0.46)
–
0.02 (0.02)
0.02 (0.02)
0.09 (0.07)
0.94 (0.84)
0.02 (0.02)
0.09 (0.09)
0.08 (0.08)
Ho (He)
Conservation Genet Resour (2015) 7:917–944 921
123
123
Cetorhinus maximus
Cmax02e,1
Cmax01e,1
Cbin66d
Cbin62d
Cbin59d
Cbin57d
Cbin56d
Cbin50d
Cbin48d
Cbin36d
Cbin33d
Cbin31d
Cbin27d
Cbin16d
Cbin15d
Cbin12d
Cbin08d
Cbin07d
Cbin05d
F: CAGCAACCTGAAGCCTGTT
Cbin02d
Chorthippus group binotatus (Orthoptera: Acrididae)
R: CAGTGCAGGGTGTGCAAG
F: TGACTTTGGCAGTTGGTTTG
R: AACATTTCAAACCTAGCTCATCTTC
F: GAAATGAATCTCACAGGTATCAGG
R: CTCGAGCTTCCATCAGCTTC
F: AAGTCCCCTCTACATCCCCT
R: GGCCCTGGTCCAATAAGAAT
F: CCTCTCGTTGTATACACTCCCA
R: TCAATTCGAACTGGACAGTCA
F: TAAATTACCTCCCCTCAATCCA
R: GCAGTGGTGTGCAATAATCG
F: CGGAGAGCACAAACACAAAA
R:CTGGACAATTCAACCGAAGG
F: AACAGCCGCCGAGATAAAG
R: ATCATCTGGCGAGCAAAAGT
F: AGGCAGGAGCTTAGAAACGTC
R: CGGCTTTCTAGCATTTGTGC
F: TTCAGAGATGAAGAGGCTTGC
R: GCTGTAACACCACGACGGAC
F: AGAGATTCCAGAGCTATGCTGG
R: CGTCAAACGTAGTGAAGTTAGTAGG
F: CACTTTGAATGATTAATCTCCTGATTT
R: ATATCGAACCACGATTCGGC
F: AACAAGTTTGGACGTTTCGC
R: AACGTTCAGTTCGATTATCCG
F: GTTCGGTTGACCGATGTTC
R: GCACCGGAGACTTGAAACAG
F: TTGCTTCGTAGCTTATCGGTG
R: ACAGCAGCCAATCTGGAGTT
F: TGAATGGGCTTTCAAAGAGAA
R: CACTGTATCATGCTGAAGGCA
F: AGAGTAGCGTGGAGAGCTGC
R: CGATGTGTACATAGAGAAATCTAGTGA
F: TCTGCACATTAATTTTGATAGGG
R: TCAGAGTGACGGCAATATACAA
F: CAAGTGGACAAACTCGAGCA
R: CAAACTTCATATTGCAACCCC
F: AGAATGAGCCACAAATTAAAACA
R: GACATGTCGGATTGGACC
Primer sequence (50 –30 )
Locus
Species
(GA)10
(TG)9
(GA)9
(TG)10
(ATC)10
(TC)10
(CT)10
(TCT)10
(ACA)10
(AC)23
(GT)22
(AC)21
(CA)19
(CA)15
(CA)14
(CCA)13
(TC)12
(GA)12
(CA)12
(TGT)12
Repeat motif
189, 191
161, 163
217–391
244–358
132–237
64–138
178–234
162–247
109–193
72–254
90–192
62–166
103–221
240–382
95–191
60–153
99–291
150–246
260–332
204–315
Size range (bp)
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
Sample size (N)
2
2
11
8
11
6
10
9
8
13
16
10
11
11
9
4
14
6
9
6
Number of alleles (NA)
0.45 (0.36)
0.40 (0.51)
0.58 (0.86)
0.35 (0.66)
0.88 (0.89)
0.44 (0.78)
0.50 (0.80)
0.83 (0.87)
0.83 (0.87)
0.31 (0.92)
0.66 (0.91)
0.50 (0.71)
0.50 (0.89)
0.21 (0.90)
0.94 (0.77)
0.38 (0.51)
0.72 (0.91)
0.64 (0.75)
0.83 (0.88)
0.35 (0.68)
Ho (He)
922 Conservation Genet Resour (2015) 7:917–944
Scapharca kagoshimensis (Mollusca: Bivalvia)
Species
Sk02f
Sk01f
Cmax19e,2
Cmax18e,2
Cmax17e,2
Cmax16e,2
Cmax15e,1,4
Cmax14e,2
Cmax13e,2
Cmax12e,3
Cmax11e,2
Cmax10e,2
Cmax09e,2
Cmax08e,1
Cmax07e,1
Cmax06e,1
Cmax05e,1
Cmax04e,1
F: CACATCAGGCCAGGTGAAC
Cmax03e,1
R: ACCAACAACAAAAATACAGT
F: ATTGTCAGCGTTTCGGTA
R: GGAACACACAAATACACATACG
F: CTTTTCATTGGGCAGTCCTTA
R: ACTCCACCCAAGTTCCATTG
F: GTCAGGCAGGTCTTGGAGAG
R: GTCACCTGGATTAAATGCCATC
F: TGAGAGAGACAAACTTTAGCTCATAAATC
R: AGGCCTGAGTGTCTCTCACC
F: TCCACACAGCGTTAGTGACC
R: TAGGCATCCCTACAGCTTGG
F: GAGCTGACTTTGGCAGTTGAG
R: TCCATCTTGTCTTTGCTTTTGGG
F: TCAAGCTCAGCCTTAACTCCG
R: GGCTGAATTGCCTACTCCTG
F: GCCTGACTGCCTTCTGTCTAA
R: TTGGTTCAAAGGTGGAGACC
F: AACCACTAGCCGAGCTGAAG
R: ATGCATGTGACTGATTGTTGG
F: TTATTATACTTTCGTCACACATACATGC
R: GTCACCAACATTACCATGTCTCC
F: CACCAATTGGCTTCATCCTC
R: GCTCTAACTGTTTCTGCCTTCC
F: GGGAAATGATGGAAAATTGC
R: ATTGTTGTGGCCCGAGTTAG
F: CCAATAGCCTCAAGCCTGTC
R: TGGCGGGATATTGTGGTC
F: CAGCACTTGGTCAGAGGTAGG
R: TTTCATGAGGGCTGGATACC
F: TGTGCTGGCCAATATTTATCC
R: GTCATCCAGGGTTTCAGTCAC
F: TGCTCGTAGTGTCTCATTCAAAC
R: TCTACCTTTCCTCAACCTCTAGTAGC
F: TTTCCATGGCCAACCTAAAC
R: GCCAAGTTGCTGCTGTGTC
F: AACCTTCCTTAAGAGCATTCTGC
R: CTGCTTGGCACTAAGCTGTG
Primer sequence (50 –30 )
Locus
(CA)26
(CT)14
(TG)11
(CA)17
(CA)10
(GA)13
(TG)9
(CA)10
(CA)13
(CA)12
(CA)13
(CA)9
(TG)10
(GA)14
(CA)21
(TG)14
(CT)11
(CA)12
(GA)9
Repeat motif
360–430
142–196
179–189
135–155
141–157
169–175
325–349
156–182
233–245
358–376
99–111
177–183
153–159
194–212
203–227
213–253
145, 147
91–105
121–127
Size range (bp)
35
35
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
Sample size (N)
8
10
4
10
9
4
8
9
7
6
7
4
4
7
10
8
2
5
3
Number of alleles (NA)
0.667 (0.829)
0.700 (0.786)
0.70 (0.62)
0.90 (0.88)
0.90 (0.84)
0.50 (0.65)
0.80 (0.81)
0.80 (0.69)
0.95 (0.80)
0.70 (0.77)
0.95 (0.80)
0.75 (0.68)
0.75 (0.65)
0.65 (0.74)
0.90 (0.88)
0.85 (0.81)
0.60 (0.49)
0.80 (0.75)
0.65 (0.62)
Ho (He)
Conservation Genet Resour (2015) 7:917–944 923
123
Species
123
Sk20f
Sk19f
Sk18f
Sk17f
Sk16f
Sk15f
Sk14f
Sk13f
Sk12f
Sk11f
Sk10f
Sk09f
Sk08f
Sk07f
Sk06f
Sk05f
Sk04f
F: GGGAATAAAGGGATAAACTC
Sk03f
R: AACCTATTGACACACCTACA
F: AACGAGTAGCTCAATAATTCA
R: TGGTAAGGTGGACCCTGT
F: AGATGTAAAAGGCACAGACAAC
R: AGTCAATTTCATCTCTGTAGG
F: ACAATCAAATGCTTACATACTTC
R: CATACAAAAAATAGACAGTGCT
F: AGAAACAGAAAAATAGTCCAAC
R: GATGTGTATTGTTTAGTGTCA
F: GTTCAAATAGCTTAGATTCCG
R: TAGGCAGACCAATCGTGAAGC
F: TGGCAAGATAGGTATGTGCAA
R: TCAAATAGCTTAGATTCCGA
F: TTATGTTTTGTTTAACCACG
R: AGAGATTGAAAGAGATTGCCT
F: TGAACGGTATAATTCCTAGCTG
R: TACAACTCTACTGCATCATCT
F: TTTGGACCACCTAGAATATGA
R: AAACGAGTAGCTCAATAATTCA
F: CCTATTGACACACCTACACAT
R: GTCACGAATGTTATACTGGAG
F: GCAACCATTATTTGGAACAC
R: TTATGCAACACTTCCAGTA
F: GTAAGCAAATTGGACATC
R: ACATAAATAGCTGGGACTC
F: AGTCAGTAATTGTAGTCTTGT
R: ATTCACCGCTGGGTATTT
F: TATTAGTGGAGTTGGTTTCGTC
R: AAACAGTTCTAACCAGTC
F: AACAACTTTGAACTATAACT
R: TTCAAATAGCTTAGATTCCGA
F: TTTAACCACGTGTAAACGAAA
R: TATACTCTCTATTTTACGCTC
F: ATAGTCACCATATTCCTACAA
R: AGATAATTTGGTAATGGTCTT
Primer sequence (50 –30 )
Locus
(CA)8(CT)7
(CA)25
(CT)22
(CT)11(CT)4(CT)13
(CT)12(CT)9(CA)4
(CT)19
(CT)16(CA)11
(CT)17(CA)6
(CA)9(CA)4 (CA)9
(CT)7(CA)8
(CT)25
(CT)9(CT)8
(CT)14
(CA)19
(CT)19
(CT)13
(CA)23
(CT)15(CT)11(CA)4
Repeat motif
245–320
341–381
132–210
270–345
358–446
220–296
368–426
300–360
406–444
280–330
164–230
395–501
224–256
282–370
385–418
425–505
220–256
223–325
Size range (bp)
35
35
35
35
35
35
35
35
35
35
35
35
35
35
35
35
35
35
Sample size (N)
11
6
21
14
10
26
10
9
11
7
10
9
8
11
5
7
9
10
Number of alleles (NA)
0.759 (0.883)
0.677 (0.821)
0.704 (0.948)
0.700 (0.914)
0.563 (0.868)
0.829 (0.960)
0.800 (0.862)
0.815 (0.850)
0.655 (0.881)
0.655 (0.808)
0.759 (0.872)
0.629 (0.867)
0.735 (0.862)
0.886 (0.886)
0.600 (0.733)
0.656 (0.825)
0.727 (0.800)
0.867 (0.856)
Ho (He)
924 Conservation Genet Resour (2015) 7:917–944
Bagrus bajad
Bd02g
Bd01g
Bd18g
Bd04g
Bd10g
BD14g
BD03g
BD08g
Bd16g
BD07g
Bd05g
Bd20g
Bd06g
Bd09g
Bd12g
Bd02g
Bd01g
Bd18g
F: TGTGGACCAAGAGACAGGTG
Bd04g
Bagrus docmak
R:GGGTATCGCATCCCAGATAG
F: TGTGCTCTGACCCCTACCTC
R:TAAAGCTGGGCAACTGATCC
F: TTGCCAATCCTGATGACACTC
R: CCTGAGTGCATTGCTCATGG
F: ATGGGGAGGAAAAGTGGAG
R: AATGAACAAGGCAGGTGATG
F: TGTGGACCAAGAGACAGGTG
R:TCAACTTCTTAGCACAAAATCAGAC
F: GTCCCACGGACTGAAAAGTG
R: CTCAAAGCGCTTGAAGTGG
F: CTTTAATGACACTGCGCTGC
R: CGTGCCATAGGCATTTATCC
F: CCTGCAGGAGTTTGTTTGTG
R: GGTAAAGGTTTACACTGTGGGG
F: TTACCTCACACTCTGGGGTTG
R: TAGTAGCGCACCCAGGAAAC
F: GCAATCGCACTCTTGTTATCG
R: TTGTAGATTCCCTTTGGGATG
F: GAGCACACGAAACATTGCAG
R: CAGCATTTCATTGCTATGTGC
F: GCTGGCAACATGCAGTAATC
R: TGCAGGTTAAGAATGGAGGC
F: TCCTGGAGACCAAGACCAAG
R: GCCCACACTATTGACACAGG
F: TTCTGAAGCCCAAAGTAGACG
R: TGGTCAACTTTAGATGTGCAGC
F: ACTGTTCCCATGAAGTTGGG
R: CTCTTCCCCAAGGCTATTCC
F: CCGACCATCTCAAATACAAGTC
R:GGGTATCGCATCCCAGATAG
F: TGTGCTCTGACCCCTACCTC
R:TAAAGCTGGGCAACTGATCC
F: TTGCCAATCCTGATGACACTC
R: CCTGAGTGCATTGCTCATGG
F: ATGGGGAGGAAAAGTGGAG
R: AATGAACAAGGCAGGTGATG
Primer sequence (50 –30 )
Locus
Species
(AGAT)17
(TTCT)15
(AC)15
(AGAT)18
(TTA)15
(TAT)17
(TAGA)15
(ATCT)16
(ATT)13
(GATA)15
(ATAC)15
(CA)11
(GATA)16
(ATT)19
(AAT)18
(AGAT)17
(TTCT)15
(AC)15
(AGAT)18
Repeat motif
110–126
190–206
125–133
197–229
266–287
218–233
159–191
179–187
83
125–157
136–172
156–168
171–199
223–238
237–258
110–130
203–219
100–102
200–208
Size range (bp)
5
5
5
5
16
16
16
13
13
4
13
16
16
16
16
16
15
16
16
Sample size (N)
4
5
4
5
7
6
3
3
1
5
4
4
7
6
3
6
4
2
3
Number of alleles (NA)
0.600 (0.580)
1.000 (0.740)
0.600 (0.480)
0.400 (0.760)
0.375 (0.426)
0.688 (0.781)
0.438 (0.568)
0.615 (0.544)
–
0.538 (0.678)
0.250 (0.719)
0.688 (0.672)
0.625 (0.609)
0.563 (0.734)
0.688 (0.508)
0.563 (0.541)
0.333 (0.589)
0.563 (0.451)
0.563 (0.646)
Ho (He)
Conservation Genet Resour (2015) 7:917–944 925
123
123
Acropora muricata (Linnaeus, 1758) (Scleractinia, Acroporidae)
Species
Am08h
Am07h
Am06h
Am05h
Am04h
Am03h,5
Am02h
Am01h
Bd10g
BD14g
BD03g
BD08g
Bd16g
BD07g
Bd05g
Bd20g
Bd06g
Bd09g
F: CCGACCATCTCAAATACAAGTC
Bd12g
R: GGCTGCTTGATCACATCTAGTTT
F: TTGCAAACAGCAAGCCAG
R: AGCAAAGTGAGGATGCCATT
F: GGAATGAGGCACAAAGAAGG
R: GCAAAGTACGGATTCTGCAA
F: CAGCACGATAGTGGACAAAA
R: CTTTTGAACCAGATTGATCGC
F: GGTCAGCAGGTAGGCGGA
R: TGGTTGTCAAGGGCAATTTT
F: GGGGAATTACTCCTCTAATTCAA
R: CGAAATGCGCTCCTTTACTA
F: GCGACCAGACAGGCTCTTA
R: ACCATGTAAACTGGGCGAAT
F: TGAAGTTACCAAAGTGCCTTAAA
R: GAGGTGGAAAATGAGTAGCAAT
F: CGTCTCGCACTGGTTTACAA
R:TCAACTTCTTAGCACAAAATCAGAC
F: GTCCCACGGACTGAAAAGTG
R: CTCAAAGCGCTTGAAGTGG
F: CTTTAATGACACTGCGCTGC
R: CGTGCCATAGGCATTTATCC
F: CCTGCAGGAGTTTGTTTGTG
R: GGTAAAGGTTTACACTGTGGGG
F: TTACCTCACACTCTGGGGTTG
R: TAGTAGCGCACCCAGGAAAC
F: GCAATCGCACTCTTGTTATCG
R: TTGTAGATTCCCTTTGGGATG
F: GAGCACACGAAACATTGCAG
R: CAGCATTTCATTGCTATGTGC
F: GCTGGCAACATGCAGTAATC
R: TGCAGGTTAAGAATGGAGGC
F: TCCTGGAGACCAAGACCAAG
R: GCCCACACTATTGACACAGG
F: TTCTGAAGCCCAAAGTAGACG
R: TGGTCAACTTTAGATGTGCAGC
F: ACTGTTCCCATGAAGTTGGG
R: CTCTTCCCCAAGGCTATTCC
Primer sequence (50 –30 )
Locus
(CA)7
(AC)7
(AG)8
(GT)8
(AC)7
(GT)7
(CT)7
(TC)7
(TTA)15
(TAT)17
(TAGA)15
(ATCT)16
(ATT)13
(GATA)15
(ATAC)15
(CA)11
(GATA)16
(ATT)19
(AAT)18
Repeat motif
147–151
135–145
114–120
137–155
146–154
162–176
195–221
313–319
265–277
225–241
179–203
179–187
83–89
134–158
125–133
148–150
203–215
240–243
236–239
Size range (bp)
46
46
46
46
46
46
46
46
5
5
5
5
5
5
5
5
5
5
5
Sample size (N)
3
5
3
3
4
5
6
3
4
3
6
3
3
4
2
2
4
2
2
Number of alleles (NA)
0.07 (0.07)NS
0.50 (0.49)NS
0.36 (0.42)NS
0.21 (0.27)NS
0.14 (0.14)NS
0.36 (0.44)NS
0.29 (0.61)5
0.50 (0.58)NS
0.600 (0.480)
0.400 (0.340)
1.000 (0.800)
0.400 (0.460)
0.800 (0.540)
0.750 (0.750)
0.200 (0.180)
0.200 (0.180)
0.800 (0.660)
0.400 (0.480)
0.400 (0.320)
Ho (He)
926 Conservation Genet Resour (2015) 7:917–944
Pelteobagrus vachelli
Porites lutea Milne-Edwards & Haime, 1851 (Scleractinia, Poritidae)
Species
F: GGACTGGAACACAACAGGCT R: CCCAAACCGCACATTATTTC F: GCTTTTTATCATGCAGTGTGG R: GAAAATTCAGCATTTCCAAGC F: CAGGCTAACCTTGGCTTGAC R: CAGTCCCAGTCAGCTCTGC F: CCGTACACAGTTGCTCTCCA R: CAAAACACTCCAGCAGTCCA F: CTGTCACACTCGCCTTCAAA R: TTACTCGCAGTGCATTTTGG
KP735111i PV11i KP735112i PV19i KP735113i PV20i KP735114i PV22i KP735115i
R: GCTGTGAGCTTTAACCGACC
F: GCTGTTCAATGCACTTCTGG
R: GAATCAACCAATGGCAGTCC
F: CACCATAATCATGAGATTTACTATTGA
R: CACGACTACCGGGTGAAGTT
F: TTGACTTTGTGGGCTTGAAA
R: CGAAGAACCGAAACAGGAAG
F: GACCGCTGATCAAAGGCTTA
R: TACTGCTTCTCAACATGCGG
F: CAACCAACCACTTCTGCTACA
R: GGTTTATAGATTCCATCACTAACCAA
F: ACGTGCAGGTTGAATGTATGC
R: CTCGACATGAGCATTGCCT
F: TGGTATATTATATTTCTGTTCCCTTTT
R: TTCAGTGTCAAAAGTGCAAGAAA
F: TTTTATGGAGTGGCCATTTG
R: GGAAAGACGAAATTAAATAGCCC
F: TTGCCCCATTCCAATAACTG
R: GAGCCGAACAGATTTCAACC
F: GTCATCGTCATCACCATCCA
R: TGGTATTTCATACATTATTTCCCTTG
F: TCATTCAATACCTTCTCAAGATTCA
R: TTGCAGCATCAAAGACCC
F: GCAATGCCATGGTTTCCA
R: CGACCAACCAATAACCACTT
PV5i
Pl11h
Pl10h
Pl09h
Pl08h
Pl07h
Pl06h
Pl05h
Pl04h
Pl03h
Pl02h
Pl01h
Am11h
F: CTCCCAACAGATGCTATTTAAGAGA
R: CGTTGATTTTGCTGACTTCAA
F: GAAGGCTCTTGTGTTGCGAT
Am09h Am10h
Primer sequence (50 –30 )
Locus
(GT)9
(AC)9
(GT)9
(GA)10
(CA)10
(AC)9
(AC)9
(AG)7
(CT)7
(TC)6
(GT)10
(TC)8
(ACA)7
(AAC)7
(ACC)6
(AG)11
(TC)9
(AG)9
(CA)8
Repeat motif
251–255
227–245
275–281
258–278
214–222
141–149
123–143
179–181
182–226
200–204
190–204
131–143
144–153
185–200
91–112
232–276
136–140
145–151
113–129
Size range (bp)
32
32
32
32
32
48
48
48
48
48
48
48
48
48
48
48
46
46
46
Sample size (N)
3
10
4
8
5
2
4
2
3
2
5
2
3
6
3
7
3
3
4
Number of alleles (NA)
0.188 (0.177)
0.844 (0.782)
0.563 (0.537)
0.938 (0.802)
0.688 (0.581)
0.32 (0.51)NS
0.53 (0.73)NS
0.05 (0.05)NS
0.05 (0.15)NS
0.05 (0.15)NS
0.58 (0.71)NS
0.16 (0.49)NS
0.05 (0.40)5
0.37 (0.59)NS
0.47 (0.54)NS
0.53 (0.74)NS
0.21 (0.20)NS
0.14 (0.37)5
0.43 (0.60)NS
Ho (He)
Conservation Genet Resour (2015) 7:917–944 927
123
Species
Primer sequence (50 –30 ) F: GCTGAGAACTGAAACCCTGC R: ACATGGACCTCCCATTGTGT F: TTTCAATGGCCTCCTGATTT R: TTCTTCCCTTTTTCCCTCGT F: GCCAAGACGACATCACAGAA R: TGTGCCAGGTTGTGTACGTT F: TCGGTCAGTTCAGATCACCA R: CACACAACACAATCTGCCAA F: GATGAAAGAAACCCGGAACA R: TGGAGAGAAAGAAGGCCAGA F: CAACGACTGGAGGTCAAACA R: AGCTGACGGAACTGTTACTGC F: TGAAATAGCTTGCCGTTGTG R: TGGATTGTTGTGAGGTTGGA F: GGGATTAGGGTGTAGGGAGC R: CCCTAAGTTGAGCGCCTTTA F: TCACTACCGGGGACTGACTT R: TAAAAATTCACCGGCCATTC F: AGCATTGGAGCCGATCATAC R: AGGCACCCTCAGTAAGAGCA F: AGCAGCCTCTTCAACCGTTA R: CAGGTAGAGTGGGAGTGATGG F: TCAAACTGGGCAAAAACTCC R: AACGAAGGGGGTTTTCAGAT F: TTTTCTGAAGGAGACGTCGG R: CCAGGCAGGATGGTTACCT F: CTTCCCAGAAGTCTGAACGG R: TCCAGGATCAGGACATCACA F: CCACTGGCAAAACATTGAAA R: TCAGACCCGCCTTAATATGC F: ATGATCAAACACAGTGGGCA R: AGAAGACGGGAGGAGGAGAG F: TGCTTCCTCTCCGCTATTGT R: GCAGCCACTATTATGAGCCC F: CTGGGAAACATCCTGGAAAA R: AACCATCCTGCAGGTGAGAC F: CACATGATCGTCACCTCGTC R: TGAGGATGATTCTGCACCTG F: TTCCCTAAATGACCTCGTGC
Locus
PV23i
KP735116i
PV28i
KP735117i
123
PV30i
KP735118i
PV34i
KP735119i
PV35i
KP735120i
PV36i
KP735121i
PV37i
KP735122i
PV39i
KP735123i
PV40i
KP735124i
PV43i
KP735125i
PV44i
KP735126i
PV45i
KP735127i
PV49i
KP735128i
PV50i
KP735129i
PV65i
KP735130i
PV71i
KP735131i
PV84i
KP735132i
PV86i
KP735133i
PV88i
KP735134i
PV90i
(GTT)6
(ATG)6
(TTG)6
(TCC)6
(CAT)7
(TCA)7
(AAG)8
(AC)8
(AC)8
(TG)8
(TG)8
(GT)8
(AC)8
(AC)8
(GT)8
(TC)8
(TA)8
(TA)9
(CT)9
(GA)9
Repeat motif
259–268
218–220
228–243
230–233
254–260
231–237
268–289
272–278
249–279
257–261
275–281
258–260
258–268
251–257
285–315
220–222
237–245
279–291
273–283
241–251
Size range (bp)
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
Sample size (N)
3
2
6
2
3
3
7
4
13
3
3
2
5
4
12
2
5
7
6
6
Number of alleles (NA)
0.594 (0.549)
0.781 (0.484)
0.938 (0.79)
0.406 (0.484)
0.125 (0.354)
0.125 (0.121)
0.938 (0.748)
0.563 (0.586)
0.813 (0.864)
0.188 (0.294)
0.344 (0.304)
0.125 (0.222)
0.75 (0.655)
0.5 (0.563)
0.625 (0.79)
0.031 (0.031)
0.438 (0.643)
0.844 (0.712)
0.563 (0.711)
0.688 (0.672)
Ho (He)
928 Conservation Genet Resour (2015) 7:917–944
Species
Primer sequence (50 –30 )
R: ACTTTCCCCCTTTACATCCG
F: CGTTCACCGTTTTTCAGAGA
R: AGATGCAGCGGTGCTTTAGT
F: TCACCTGCCGATTTCTATCC
R: TGCAAATGAACAGAAGCAATG
F: GAACTGTACGGCACACTGGA
R: TTTGATCGTTGCTTTCTTTGTC
F: GCTTTGTCGTGATTTGAGCA
R: CTCATGTGGAATGAACGCAC
F: GGGCATGCTGAGGAACTAAA
R: GGTTTAAACACCTGTTCCATCA
F: GGAGCTGCCTGTCTATCTCG
R: TGGACTGAAAAACTACAGGAAAA
F: AGAAGTGCTGCACACCAATG
R: TCCTGCCACTTGTCTAAGGAA
F: AGCGTGCTGGAGTATTGCTT
R: CGGTTCAGAGGTTCACTCGT
F: AGTCACAGACGCAGAAGCCT
R: TAGCCGGAGTTACCAAATGC
F: AAACGCCAAAGAACCTGATG
R: CTGACAAGACTGCAGCAAGC
F: GGCTGGTAAAATGGTGGTGT
R:GAACAGTGCATGCAGGCTAA
F: GGCTAGCGGCTCAATTACTG
R: CTCCTGGGACATACACACCC
F: TAATGAGCGGAACAGGGAAG
R: ACACACAACTCACACCACCC
F: CAGACAGGAGGTGTGTGTGG
R: GCAGCTGTCAGTCATGTGGT
F: CGCTGAACTACACACACGGT
R: ATCAGAGCCCATGCTGAGTT
F: TATGTGGTGGCCATGTCTGT
R: TCAGACGGGAATTTGTCCTC
F: TGCTAGCAATTAGCACTGGG
Locus
KP735135i
PV92i
KP735136i
PV93i
KP735137i
PV97i
KP735138i
PV98i
KP735139i
PV102i
KP735140i
PV103i
KP735141i
PV111i
KP735142i
PV114i
KP735143i
PV117i
KP735144i
PV120i
KP735145i
PV122i
KP735146i
PV123i
KP735147i
PV128i
KP735148i
PV136i
KP735149i
PV139i
KP735150i
PV140i
KP735151i
PV141i
(AC)7agacaaacacacacacat(AC)6
(TG)6cgcacgcgcaagtgtgcaaccccta tgtgaatgtccactgtgttgcagattttaagc(TG)7
(AC)7aaaaaaaa(AT)6
(G)11cagatgtgggcaagaattcatcct ccctcagc(TG)8ttggactcgcct(TG)6
(TG)7tacagggttgtgtgttggtgt gtgtacagggtggggtgtgctat gattgttgtgtgtgtactggatggtgtgagt(TG)6
(CA)7cgttttccttttttatgatac agtacggccacagcagggtga(GT)6
(AT)8aaa(GT)7
(TCCA)5
(TAGC)5
(AAAT)5
(ATGT)5
(GGT)5
(ATT)5
(TCT)5
(TTA)5
(AAT)6
(ATG)6
Repeat motif
279–283
251–255
288–294
273–293
226–244
254–280
282–288
231–247
249–269
281–289
248–264
262–274
232–244
268–289
250–253
249–255
222–240
Size range (bp)
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
Sample size (N)
3
3
4
6
5
8
4
4
6
3
5
5
5
7
2
3
7
Number of alleles (NA)
0.438 (0.405)
0.531 (0.493)
0.531 (0.659)
0.719 (0.686)
0.563 (0.61)
0.844 (0.769)
0.75 (0.614)
0.625 (0.58)
0.531 (0.605)
0.281 (0.466)
0.375 (0.354)
0.594 (0.667)
0.469 (0.785)
0.844 (0.728)
0.125 (0.119)
0.656 (0.631)
0.938 (0.801)
Ho (He)
Conservation Genet Resour (2015) 7:917–944 929
123
123
Sebasticus marmoratus
Species
F: GCGAGAGAGCCGTTATTTTG R: ATCTCTTTTGGCCTTTGCAC F: TGCACTTGAAGATCAGTTGTGTT
R: TCGATAATTCATGATTGCCA F: GGGAGAGAAGAAGGGAATGG R: CGAAAGACAAAATGGAGGGA F: AAACGTACCCACAGAGCCAC R: TCCAAAAATGGTGTCGTTCA F: TCAATACACTCGCTGACCCA
R: AGCGTCCGAACAGAACAATC F: TCCTGGTACACTTTCGCACA R: TGGATGGCGATCATACTGAA F: CACTTGCACTTCTCGTGTGA R: TAAACAGCCTCACCAAACCC F: TAGTGTTCCTGTGAAGCCCC
R: AGGGAGAGCGTTTGTGCTAA F: TCTCCGGCGTTGTAGAAGTT
R: TGGGAATCCTCGTGATTCAT F: TGTCTGCGTGTGTACCTGTG R: ACGGCTCTGCTTCAGTCTGT F: GTGAATTGGGTGAGTGGCTT R: GCCAAGTGGTGAAGGGATTA F: CAAACTGAGCAGGTTTGTGTAA
PV147i
KP735153i
PV148i
KP735154i
PV153i
KP735155i
PV155i
KP735156i
PV156i
KP735157i
PV165i
KP735158i
PV169i
KP735159i
PV173i
KP735160i
PV175i
KP735161i
PV177i
KP735162i
PV184i
KP735163i
Sm31j
Sm49j
Sm43j
Sm38j
R: GGCATCAGGGTTTCTGGTTA
KP735152i
R: ACGAATGGCTTCAGTTCAATCT
F: GCTGTTATCAGTGATCCTGGAG
R: GGTGGTGGATTGGTGTTAATCT
F: CAGTTTGTAGTCAGCTGTCTGTG
R: TTTAAAAGCGGGCTCAATATGT
F: GTCGTTGACAGAGCTTGACTTG
R: AACTCTCTCCCCATAGAGGTCC
Primer sequence (50 –30 )
Locus
(GAA)10
(TAA)11
(CTT)18
(ATA)19
(GT)6(TG)6
(GT)8aagtttgtatgtccatgtgtcagtgtgt ttgtccagctattcctatgtc(TG)6
(GA)7atcacgtgagggttctgtcagctgcgcttcactcc tagctcaggggcagctgtgttacgtaaacaagt cggagatgaatgatttgtgttt(AC)8
(AGC)5atcctcacagtgctgatgcgttcagtgtgcg actggtcctgagcggcagcagcattacgatcctaa cgtgataaactgagcaccgtctggtctggg(GT)9
(GAA)5aaaaa(AT)6
(TGT)5tttgtttgtttttagct(CAG)5
(TG)6ttgtgtattagataataaagcaaaaataat aaggatccagaaaagtgtgggggtttaaata gaaataagg(ATA)5
(GT)9cctcaggttagaagtacttcaac(CA)6
(TC)7catcgctccaagtgcttcctcccca(TTG)5
(GAT)5gtaatttaaatgtgtttctttaaaaattcttg agtcaagcctaaaactaaggtacaataaatac(TG) 7cgcgtccgtgtgtatgtgtgtattatagaatta(AT)8
(TG)7cattttcttttttctgtatggaaattgttac tgttttatgtgtgtgtaaatcctgtatttaaatc(CA)8
Repeat motif
120–160
150–180
230–270
230–260
218–222
275–285
268–271
272–297
290–293
274–280
289–294
291–308
279–282
279–296
232–240
Size range (bp)
24
24
24
24
32
32
32
32
32
32
32
32
32
32
32
Sample size (N)
6
5
5
4
3
4
4
8
4
3
3
5
3
5
5
Number of alleles (NA)
0.556 (0.770)
0.250 (0.742)
0.556 (0.748)
0.917 (0.727)
0.563 (0.5)
0.625 (0.552)
0.813 (0.648)
0.656 (0.821)
0.656 (0.741)
0.625 (0.628)
0.469 (0.458)
0.469 (0.628)
0.219 (0.35)
0.406 (0.357)
0.5 (0.66)
Ho (He)
930 Conservation Genet Resour (2015) 7:917–944
Species
Sm290j
Sm289j
Sm216j
Sm198j
Sm168j
Sm154j
Sm149j
Sm148j,****,
Sm144j
Sm131j
Sm124j
Sm117j,****,
Sm106j
Sm102j
Sm101j
Sm95j
Sm94j,****
Sm92j
F: ATCATCAACATATCGGAGCCTT
Sm58j,
R: TTGAATTCAGTGCATTTTAGGA
F: TGATCGACTGCAATATTTACGG
R: GGAACTGTGATGCTGAAACTTG
F: TGGTTGAATACATGACGAGAGG
R: TTGTTTTGTACAGTGACGTGGC
F: ATGTTGTGTGACTCCCGTAATG
R: GTGAGTGGGTGTGTGTGTTTTT
F: TCTCAGAAAGGTGCGTCACTTA
R: CACATCATCAAGATCGTGTCCT
F: GCAAGTGTGGAGCTAATCAGTG
R: CAGGGTCAGGATCTTTGAAAAC
F: ACTTGAATTTCCCTCTGGATCA
R: ACTGTGATGCTGGAAACTTGAA
F: AGTCTGGATAGGAGGGGTTAGG
R: TCTTCCGGTCATGTACAACTCT
F: TTTAGCATGGTGGTTTGAAATG
R: GCATGCAGAAAAGAGAACTTGA
F: AGTCAAAGGAATGGTCTGCAAT
R: AGGAGCAACGATGCATTTTATT
F: TCATTCAAGGTTTGTAGCTGGA
R: TGGGCCATCACAATAACAGTAG
F: AAGCGCATATTTTTCCACCTAA
R: TGCAAGTTGTCCTCTGTATGCT
F: ACATGGTCCGTAGCAGAGTTTT
R: ACTGTGGACGGTCTGTACAATG
F: GATTGCGTTAATGCGTTGAATA
R: CTATGGAACTGTGATGCTGGAA
F: AGACACTCCCACCTCCTACAGA
R: ATATGTAAAGATGCTCAGCGCC
F: GTGAGCTCTTCTGTTTTGGGAG
R: ATATGTAAAGATGCTCAGCGCC
F: GTGAGCTCTTCTGTTTTGGGAG
R: ACGGTGTCAGTGCAGTTAAGAA
F: GTGAAAGCTGTCAGAAGACACG
R: TTCGATGTCTGCTCAGCATTAT
F: GGACTGATCTCCTTTAAGGTTCA
R: GAGACTCGTGACATTGAGTTGC
Primer sequence (50 –30 )
Locus
(TAG)9
(TAGA)14
(GTGA)16
(GAA)19
(GTT)12
(TCTA)18
(TATC)20
(AAAC)12
(ACA)18
(ATT)18
(TTA)18
(AC)26
(TAGA)16
(AGAT)12
(GGA)14
(CTT)15
(TGA)10
(TTA)15
(TTA)14
Repeat motif
260–320
220–260
220–280
200–240
180–240
160–200
110–150
110–190
230–260
120–160
120–160
220–270
220–350
190–240
180–220
110–140
140–180
180–220
130–170
Size range (bp)
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
Sample size (N)
5
6
4
4
6
6
6
7
6
5
9
4
9
11
4
6
4
2
6
Number of alleles (NA)
0.639 (0.760)
0.667 (0.835)
0.667 (0.705)
0.528 (0.654)
0.556 (0.740)
0.361 (0.758)
0.695 (0.798)
0.723 (0.806)
0.722 (0.766)
0.667 (0.766)
0.861 (0.823)
0.500 (0.713)
0.889 (0.813)
0.833 (0.896)
0.242 (0.667)
0.472 (0.745)
0.708 (0.724)
0.306 (0.497)
0.611 (0.734)
Ho (He)
Conservation Genet Resour (2015) 7:917–944 931
123
123
Aquila chrysaetos japonica
Species
AQJ62k
AQJ59k
AQJ56k
AQJ53k
AQJ52k
AQJ49k
AQJ41k
AQJ40k
AQJ36k
AQJ34k
AQJ30k
AQJ28k
AQJ27k
AQJ22k
AQJ19k
AQJ10k
AQJ08k
AQJ03k
F: ACCAACTTCTCCCTCTTTCTCC
Sm309j
R: TCCACAGAGTGCTAGCAAGA
F: CCTGTGAAAATGCATAGGTG
R: GAAAGGTGGCAAAGAGGTAA
F: GGCCAACCAGATATTAGACG
R: CAAAATTACCACTAGGAAAGCA
F: AATATATTTATTGGCACATGCAC
R: TGACCTAGATTTTCTCCAGCTT
F: TTGTTTTCATTGTGAAATACCTG
R: TGAAAGTTGATGGCAAAAGA
F: GACTGTGAGGACAGGGTCTC
R: AAACTAACAGCAGCAGCAAA
F: AGAAGCGATACGCATACACA
R: TTGCAAGCTAAAGCCTTTCT
F: AGATAGGTTGGCAAAGATGC
R: TAAAGAAGCCATTTGGGAAA
F: GGATGCTAAAGTTAACACACACA
R: ACTTTTAGACCAGCCTGACG
F: TCCCAGTAAAATCACTGCAC
R: CAAGTGTCCACTAGCACAGC
F: TGGCCACTGATTTCTATGTG
R: GTCAATCAGGTTGTACGTTAGTATC
F: AAAGGTTTGGATAATCTGGATG
R: CACGCACTGTAAAACAAAGG
F: CACTCTTGCTATAGGCTCACC
R: TGTGCCTGAATAGTGTGCTT
F: GAGACCTGAGGATTGGTGAC
R: TGGACTCCTTCTCAAAGAGG
F: TGGTGAAACCATTCTCTTTTC
R: TGATTTGAGCTGTCCCTTCC
F: TCATCTTCTTTCCCCACCAG
R: CATCGTGCTCGCTTAGATCA
F: GAAAACATCCAGCACCATCA
R: TGTCTGGAGCTCTGCCTGAT
F: GGCCTTGCCTATTCTTCTCC
R: TTTGGGCTAGAGAGGGATGA
F: GGCTTGTAAATTTGTATGGCAGT
R: ATGCAAAAGTAGACCTTGCACA
Primer sequence (50 –30 )
Locus
(AC)12
(AAGG)11
(AT)11
(GT)12
(AC)12
(AC)11
(AC)11
(AC)11
(GT)12
(GT)13
(AAT)13
(AC)13
(GT)14
(CTTT)13
(AC)17
(ACT)17
(CTT)16
(CT)11
(GT)20
Repeat motif
172–180
184–200
154–158
140–152
168–174
215–221
174–176
250–252
185–195
157–165
149–158
182–186
174–184
208–228
170–178
193–220
173–182
147–155
340–380
Size range (bp)
21
22
26
26
21
22
20
21
22
22
30
27
23
21
21
25
23
20
24
Sample size (N)
5
3
3
3
4
4
2
2
6
4
4
3
4
4
3
7
4
4
3
Number of alleles (NA)
0.381* (0.594)
0.591 (0.600)
0.538 (0.514)
0.692 (0.644)
0.714 (0.659)
0.182*** (0.449)
0.550 (0.439)
0.238* (0.500)
0.727 (0.744)
0.364* (0.402)
0.433* (0.518)
0.222 (0.313)
0.522** (0.678)
0.571 (0.573)
0.571 (0.632)
0.440 (0.544)
0.304 (0.387)
0.600 (0.569)
0.556 (0.497)
Ho (He)
932 Conservation Genet Resour (2015) 7:917–944
AQJ66k
F: GCGATCAAATAAATCCCTCT
AQJ63k
R: AGAGCTTGCTTTTCAGAACC
F: AGCAAAGTGCAGCATCAATA
R: GCGTTTTATCAAGCGTCTTT
Primer sequence (50 –30 )
Locus
Araucaria rulei
MP4
Araucaria rulei
MP3
Araucaria rulei
MP2
Araucaria rulei
MP1
SSR35l
SSR21l
SSR9l
SSR62l
SSR31l
SSR27l
SSR4l
SSR56l
SSR23l
SSR16l
SSR53l
SSR13l
SSR11l
SSR2l
R: TCCGACAACTTCTATCCCATTC
F: TCAATTCCAACCGAGTAGGTG
R: TTTCTTGGGACTTGTCGGAAATA
F: ACTTGCCTCTCCTAATGGTTGGT
R: CAGGTTATTCCATTCCCTAAA
F: TAAAACACCTAAAACCCGACG
R: TATCGATCGGTCGCATAGG
F: TGGGTGCCATATTCACTTCA
R: CGGCAAGTCTGTTCCAGAGTA
F: AAGTTCAATTGGCCGAGATG
R: CGATTCTCATAAAAAAAAATATAGAAAAAA
F: TCCATTTACAGATCATTATCGAAG
R: GCAGCTGTAATTATTACAATAAAAAAA
F: GGCTATCCCAATTTGACCAT
R: GTAGTTAGCGATTCTTTTCTCTATTTTTTTT
F: CAAAGAGGATGCCAATTTGTT
R: CAGTTTTTGAAATAGGAATATTTTTTTTTTTTTT
F: TGATTTTCTGGTGCACAATACC
R: GGATTCAATCAATTTATTAATCTAAAAAAAA
F: TTCTACAAATCAATATCTGCCCTTA
R: GCGATCTTTAAGCGATCAGTG
F: CCATTGGGGATCTATTTTTCG
R: GGAGACAACCAGTAAACAAAAAAAAA
F: GTGGTACGAGCGATCCATTT
R: GAAAGGTGGTGAATTAAAAATAAAAAA
F: GGAATGGTTATTTGATCAAGGAGAT
R: CCTGGCAAAAGATACAGAATAAAAAAA
F: CGTCGGATGGAGTGGATTAT
Araucaria rulei chloroplast microsatellites
Species
(T)9(T)11
(C)9
(T)12
(TA)9(T)9(A)11
(TA)14
(T)9(T)9
(T)11
(A)19
(A)16
(T)13
(A)9
(T)9
(T)10
(T)9
(GT)12
(GT)12
Repeat motif
262–263
222–223
170–172
221–224
258–262
204–206
147–148
238–241
219–220
337–338
253–255
124–126
203–204
253–255
154–158
184–194
Size range (bp)
28
28
28
28
28
28
28
28
28
28
28
28
28
28
22
23
Sample size (N)
2
2
3
4
3
3
2
4
2
2
2
3
2
3
3
2
Number of alleles (NA)
(0.245)
(0.069)
(0.518)
(0.625)
(0.349)
(0.401)
(0.069)
(0.651)
(0.459)
(0.459)
(0.500)
(0.446)
(0.337)
(0.472)
0.545* (0.615)
0.435 (0.476)
Ho (He)
Conservation Genet Resour (2015) 7:917–944 933
123
Locus
123
Cyprinodon tularosa
MP2
MP1
Ctul_12m
Ctul_09m
Ctul_06m
Ctul_05m
Ctul_03m
Ctul_02m
37789l
33936l
12152l
9996l
9465l
9164l
8816l
3956l
3785l
3612l
2821l
572l
Araucaria rulei nuclear microsatellites
Species
R: GAAGTCAATTGAAAGTCAGCATAAGG
F: AAGCAGGAGCTAGATTGCCG
R:TTTACAGAGATACATGCCCAGG
F: CTCTGTGAATGGTTGGATACG
R: AACCCAGACAGATTAAGTGGG
F: AAGTAAAGTCACATCATACTGGTGC
R: TGTTAAGTTATCCTGCATTAAACCG
F: GCAATTTGCCTGTACAAGAGAGG
R: CCTCTAAACACCAGAATTTCCC
F: CCCTCTTACTCCGATTCCC
R: AGCCTTGCATTTCCTGTTCC
F: TCTCTACAATTACACAATTACAGTTTCGG
R: GAGTCTTGTAAGAGAGCCAAGC
F: 6-FAM-GACCCATTGTTGTGTGTTTGTG
R: TCTTGGAGTTGGGATGACCG
F: NED-AGCTTCTAATCCAATCCATATCATTCC
R: ACACTCACTATTCACTTAGCTACC
F: VIC-ACAGTGTCTTGCTCTCCTTTATG
R: AGGGTTGGACATCGTTGAGC
F: VIC-TGTGGGTAAGTGCATGAGGG
R: CCTCTTCCTCTCGGCCTTAC
F: NED-AGGATGAAGGTTGGAGACCG
R: TGCTTGTAGTTGAGAGTGAGTGAC
F: PET-TCCCAAAGATACACCCCTTG
R: CTATACACCAAGGCCTCCTC
F: PET-TTGTGGCTTATTGTGCCCTC
R: TCTCTAGAAGAGGTGCAGGC
F: NED-ACTTCTCCACACTCTGATTCTAC
R: ATGTCCACCGCATTACAAGG
F: PET-ATTTTTCAAGGGTGGGGGAC
R: ATGGATTTCATGCCCCAACC
F: VIC-ATGGTGGTGATGTGCCAAAG
R: TGTGCAGGTGGAATGCAAAG
F: 6-FAM-GGAAGAGCTTGAACCACTCAAAG
R: AAGATCGAGCCCACTCCAAG
F: VIC-ACACACACGCATAAGAAACTAAAG
Primer sequence (50 –30 )
AGTC
ATGG
ATCT
AAAG
AATAG
AATAG
(GT)11
(GT)12
(GA)12
(GT)14
(GA)12
(AC)17
(AC)12
(CA)11
(GT)11
(AC)12
(AC)22
(AC)11
Repeat motif
307–359
231–263
217–253
195–418
250–285
252–280
130–159
73–139
186–267
79–124
219–245
70–130
142–211
99–143
214–241
85–107
132–185
151–182
Size range (bp)
50
50
50
50
50
50
29
29
29
28
29
28
28
29
29
29
29
29
Sample size (N)
13
7
10
27
8
7
4
8
10
9
6
9
10
6
2
5
9
6
Number of alleles (NA)
0.88 (0.844)
0.7 (0.754)
0.354 (0.861)
1 (0.943)
0.72 (0.783)
0.88 (0.789)
0.759 (0.644)
0.862 (0.802)
0.552 (0.804)
0.464 (0.776)
0.517 (0.729)
0.857 (0.817)
0.571 (0.819)
0.690 (0.622)
0.172 (0.158)
0.655 (0.738)
0.862 (0.809)
0.483 (0.730)
Ho (He)
934 Conservation Genet Resour (2015) 7:917–944
Pinus yunnanensis
Species
PyTr05n
PyTr04n
PyTr03n
PyTr02n
PyTr01n
Ctul_45m
Ctul_42m
Ctul_41m
Ctul_40m
Ctul_39m
Ctul_38m
Ctul_34m
Ctul_33m
Ctul_29m
Ctul_23m
Ctul_21m
Ctul_20m
Ctul_16m
F: TCTAAATGTCCAATGCTGGC
Ctul_15m
R: ACCACGATACCACATTAATGACC
F: CTCAGATTTATGCCCAAGCTTCT
R: TATCATCTCACTACGAAGGGGTG
F: GGAACCATAAACAATCCAAACAA
R: AAGTGTTCGTGCAGAGAGAAGAC
F: GCGTACTGTTGTCTGTTTTCCAC
R: ACCTTCATCGACAGTGTTGTTCT
F: ATGACTTCTCCAAATGCTCAGTC
R: CATTTAAAACAAACACCTCCTGC
F: GAACATTCCCAAGACAAATACCA
R: GGGAAAGAGAGAGTGACGGG
F: GGGATTTGTGTGAAATAATGTGC
R: AGTTTAATTGTTATAGATACTTGGCCC
F: TCTAGCGTCAAATCATAGTTACTGC
R: CAGATTCAACAGCATTCATCCC
F: TTCAAGACATGAAACAACAGGG
R: CCCACTTGGGATAAGAGTAAAGG
F: TGTGGTGTGTTTCTAATGTAATTATACC
R: AATGCCATCACATCAAATGC
F: GCTGTTGTGATGCACTGAGG
R: CCATTTAACACATAGCGCAAAGC
F: CACCTACTGATCCTTGTCTAGAACCC
R: GTGTGCAAACAGGGACACG
F: AAATACTGCACGTTAGCAGGG
R: GCTGACAACCTCGGATAACG
F: TGTGTTTGTCTGTCTGTGTAAAGG
R: CAACTTAACCATCAATTTCAACGC
F: AAACTTCAACAATATGAGTCTCTGTGG
R: GCCTCTTGAATATTGAATCTGTGC
F: TACGCTTGCGACTCAACCC
R: TTTGCCCACAGTTACAAATACC
F: ACACATGTAAACACCCTGTGC
R: GGTGAAGCATTGTTTACCTTCC
F: CCCAGTTGAGATGTTAGGACG
R: GTTGGAGTATTCACGCTCCG
F: CACCTACCAGGTCTTACAATGGG
R: TTCATTTCTTTGTGTTCAGTTCC
Primer sequence (50 –30 )
Locus
AGAT
GGT
GCC
ATG
GGA
ATCT
AAAG
TCTG
ATGG
ATCT
AATC
AATC
ATGG
AACT
ATGG
AATC
ATCT
ATGG
AATC
Repeat motif
124
115–163
115
100–145
108
400–464
190–217
146–163
123–143
257–394
204–253
341–369
196–235
159–204
129–225
263–295
141–238
264–333
190–215
Size range (bp)
24
24
24
24
24
50
50
50
50
50
50
50
50
50
50
50
50
50
50
Sample size (N)
1
12
1
6
1
15
8
5
6
25
5
8
10
10
12
8
16
15
8
Number of alleles (NA)
0.000 (0.000)
0.167 (0.662)
0.000 (0.000)
0.750 (0.688)
0.000 (0.000)
0.92 (0.905)
0.84 (0.795)
0.54 (0.553)
0.64 (0.739)
0.98 (0.942)
0.74 (0.745)
0.88 (0.81)
0.8 (0.796)
0.86 (0.784)
0.88 (0.819)
0.5 (0.616)
0.96 (0.903)
0.84 (0.825)
0.58 (0.606)
Ho (He)
Conservation Genet Resour (2015) 7:917–944 935
123
Species
123
PyTr24n
PyTr23n
PyTr22n
PyTr21n
PyTr20n
PyTr19n
PyTr18n
PyTr17n
PyTr16n
PyTr15n
PyTr14n
PyTr13n
PyTr12n
PyTr11n
PyTr10n
PyTr09n
PyTr08n
PyTr07n
F: ATGCTGGTGACATTAAATCCAAG
PyTr06n
R: CTGGATTCATTTGTGGCTAAGAC
F: CAAAACCCAACGAATTAAGACAG
R: CTCTTACAGGCTGTGGAACCTCT
F: CTAAGTGTGGAAAGTTTGTTGGC
R: AACCAATGTGTAGCGAGTATGGT
F: GGGGTTATCAAAGAACGAGACTT
R: GTTGGAAGAATGGTTGCAGATT
F: CAAGCAGGAGAGTCAATCATTTC
R: AAAATGTGGCCACTGGTACTAAA
F: CATCTTCATCTTCATCATCATCCT
R: CCAGAGGGGTATCCATAGGTAAG
F: GGGGTTATCAAAGAACGAGACTT
R: AAATTACCAGCAAAACCCAAGTT
F: GAATAGGTAAGGGAGTGAAAGGG
R: TGGAGAGGATTGCTGAGATACTT
F: ACAGCAACATTTAAGTCAGCGTT
R: ATTGCCTAGTTCGTTCAATGC
F: AGGAGGAGGACGATGATGTG
R: ACAAGAACCATGATAAATCGCTG
F: TGAAAGCGAAGTAGCTTTGGTAA
R: TATCCCTTTATTGCCCTATCACC
F: CATCACTTGGGTATCTCTTTGGA
R: AGCTAATAGCAGTGAGCTTCTGG
F: TCATACAGTGGATGTTGGAGAAC
R: CTTGAAAGTTGAGGAGTCTTTGC
F: GAGCTTCTATTTCCTTTATCGGC
R: CAGTGAAGGCTCGTATAAAGGAC
F: TGTAAGTTTGTCCAAGAACCTGC
R: AAACTGAGAAACTTCTGGAAGGC
F: AATTCTCATTCTCTCCTGCAGAC
R: CAGATTCCATCATAATAGCAGCC
F: AGAGAATTAGCCAGATGATGTGC
R: TGTAAGGTATGTCGAGGAGCATT
F: CCAATGAATGTACTGCTAGGACC
R: GAGCTGTTGACACCCATACTAGC
F: ATGTTAGAGCAACACAGGAGAGC
R: ATCACAATATCTTCTGCTGCGTC
Primer sequence (50 –30 )
Locus
AT
TA
GGA
TCA
TCA
GGA
GAG
CAG
GGA
TCC
ATAG
CTG
TTC
TTAT
GAA
GAG
ATG
TGA
AAG
Repeat motif
140–156
165–185
156–186
107
143–176
158–167
134
143–152
160
173–176
155
164–194
162–174
146
152–161
153–162
135
147–201
138–147
Size range (bp)
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
Sample size (N)
6
6
10
1
5
3
1
4
1
2
1
5
5
1
4
4
1
7
4
Number of alleles (NA)
0.458 (0.761)
0.792 (0.731)
0.792 (0.800)
0.000 (0.000)
0.375 (0.326)
0.333 (0.312)
0.000 (0.000)
0.167 (0.157)
0.000 (0.000)
0.875 (0.492)
0.000 (0.000)
0.833 (0.592)
0.208 (0.355)
0.000 (0.000)
0.125 (0.259)
0.208 (0.261)
0.000 (0.000)
0.542 (0.678)
0.458 (0.647)
Ho (He)
936 Conservation Genet Resour (2015) 7:917–944
Pinus yunnanensis
FN
Species
PyTr19n
PyTr17n
PyTr15n
PyTr13n
PyTr12n
PyTr10n
PyTr09n
PyTr07n
PyTr06n
PyTr04n
PyTr02n
PyTr32n
PyTr31n
PyTr30n
PyTr29n
PyTr28n
PyTr27n
PyTr26n
F: GAGAATTTATTCAGTGCCCGTAA
PyTr25n
R: CCAGAGGGGTATCCATAGGTAAG
F: GGGGTTATCAAAGAACGAGACTT
R: TGGAGAGGATTGCTGAGATACTT
F: ACAGCAACATTTAAGTCAGCGTT
R: ACAAGAACCATGATAAATCGCTG
F: TGAAAGCGAAGTAGCTTTGGTAA
R: AGCTAATAGCAGTGAGCTTCTGG
F: TCATACAGTGGATGTTGGAGAAC
R: CTTGAAAGTTGAGGAGTCTTTGC
F: GAGCTTCTATTTCCTTTATCGGC
R: AAACTGAGAAACTTCTGGAAGGC
F: AATTCTCATTCTCTCCTGCAGAC
R: CAGATTCCATCATAATAGCAGCC
F: AGAGAATTAGCCAGATGATGTGC
R: GAGCTGTTGACACCCATACTAGC
F: ATGTTAGAGCAACACAGGAGAGC
R: ATCACAATATCTTCTGCTGCGTC
F: ATGCTGGTGACATTAAATCCAAG
R: TATCATCTCACTACGAAGGGGTG
F: GGAACCATAAACAATCCAAACAA
R: ACCTTCATCGACAGTGTTGTTCT
F: ATGACTTCTCCAAATGCTCAGTC
R: AGGCCCTTTAATGCACTAGACAC
F: CGATAGTTTAGTTTGGCTGAGGA
R: AGAACAGTCTGGACATCATGGTT
F: AAAAGCTCATCATGCATTCTTTC
R: GAGAGAATGAAGAAGTTCACCCA
F: GGAGAATTCAAACACGAGAAAGA
R: ATCAGATATGGAGTTGTAAGCCT
F: CCTACACCAGCTCCATTTTATCT
R: GTTGTATGTTTATGTGCAGCGTT
F: AGAAAAGTTTTGGTTGTGACACG
R: CAAGTCTTTTTACCGTGTAAGGTG
F: GCATTGTGAGGGGTTTCTTAAAT
R: ATATATCCCCACGGTTCTTTACC
F: AAGAACTTGACATTTTGAACCCA
R: AAACAAGCCAAACAAAAATGGTA
Primer sequence (50 –30 )
Locus
GGA
CAG
TCC
CTG
TTC
GAA
GAG
TGA
AAG
GGT
ATG
AT
AT
GA
TA
CA
AT
TA
TA
Repeat motif
164–167
149
173–176
170–173
165–171
152–158
156–162
147–201
141–147
115–163
103–145
160–168
126–134
153–167
158
156–162
125–147
124–132
150
Size range (bp)
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
Sample size (N)
2
1
2
2
3
2
3
4
3
6
3
5
5
7
1
4
11
5
1
Number of alleles (NA)
0.500 (0.375)
0.000 (0.000)
1.000 (0.500)
0.833 (0.486)
0.333 (0.403)
0.167 (0.153)
0.333 (0.292)
0.667 (0.722)
0.333 (0.500)
0.500 (0.694)
0.667 (0.611)
0.875 (0.698)
0.625 (0.571)
0.500 (0.796)
0.000 (0.000)
0.542 (0.459)
0.250 (0.805)
0.750 (0.577)
0.000 (0.000)
Ho (He)
Conservation Genet Resour (2015) 7:917–944 937
123
123
Pinus yunnanensis
KM
Species
PyTr13n
PyTr12n
PyTr10n
PyTr09n
PyTr07n
PyTr06n
PyTr04n
PyTr02n
Mean
PyTr32n
PyTr31n
PyTr30n
PyTr28n
PyTr27n
PyTr26n
PyTr24n
PyTr23n
PyTr22n
F: CATCTTCATCTTCATCATCATCCT
PyTr20n
R: AGCTAATAGCAGTGAGCTTCTGG
F: TCATACAGTGGATGTTGGAGAAC
R: CTTGAAAGTTGAGGAGTCTTTGC
F: GAGCTTCTATTTCCTTTATCGGC
R: AAACTGAGAAACTTCTGGAAGGC
F: AATTCTCATTCTCTCCTGCAGAC
R: CAGATTCCATCATAATAGCAGCC
F: AGAGAATTAGCCAGATGATGTGC
R: GAGCTGTTGACACCCATACTAGC
F: ATGTTAGAGCAACACAGGAGAGC
R: ATCACAATATCTTCTGCTGCGTC
F: ATGCTGGTGACATTAAATCCAAG
R: TATCATCTCACTACGAAGGGGTG
F: GGAACCATAAACAATCCAAACAA
R: ACCTTCATCGACAGTGTTGTTCT
F: ATGACTTCTCCAAATGCTCAGTC
R: AGGCCCTTTAATGCACTAGACAC
F: CGATAGTTTAGTTTGGCTGAGGA
R: AGAACAGTCTGGACATCATGGTT
F: AAAAGCTCATCATGCATTCTTTC
R: GAGAGAATGAAGAAGTTCACCCA
F: GGAGAATTCAAACACGAGAAAGA
R: GTTGTATGTTTATGTGCAGCGTT
F: AGAAAAGTTTTGGTTGTGACACG
R: CAAGTCTTTTTACCGTGTAAGGTG
F: GCATTGTGAGGGGTTTCTTAAAT
R: ATATATCCCCACGGTTCTTTACC
F: AAGAACTTGACATTTTGAACCCA
R: CTGGATTCATTTGTGGCTAAGAC
F: CAAAACCCAACGAATTAAGACAG
R: CTCTTACAGGCTGTGGAACCTCT
F: CTAAGTGTGGAAAGTTTGTTGGC
R: AACCAATGTGTAGCGAGTATGGT
F: GGGGTTATCAAAGAACGAGACTT
R: AAAATGTGGCCACTGGTACTAAA
Primer sequence (50 –30 )
Locus
CTG
TTC
GAA
GAG
TGA
AAG
GGT
ATG
AT
AT
GA
CA
AT
TA
AT
TA
GGA
TCA
Repeat motif
170–194
162–174
158–161
156–162
147–171
138–147
118–163
103–145
160–166
126–128
153–163
158–160
131–145
126–128
140–144
177–185
156–186
143–176
Size range (bp)
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
Sample size (N)
3
4
2
3
4
4
3
3
3.3
4
2
4
2
6
2
3
4
8
3
Number of alleles (NA)
0.667 (0.486)
0.333 (0.417)
0.167 (0.153)
0.167 (0.292)
0.667 (0.583)
0.500 (0.708)
0.000 (0.500)
0.500 (0.486)
0.556 (0.500)
1.000 (0.722)
0.833 (0.486)
0.500 (0.583)
0.500 (0.375)
0.167 (0.792)
0.833 (0.486)
0.333 (0.486)
0.833 (0.694)
1.000 (0.847)
0.333 (0.292)
Ho (He)
938 Conservation Genet Resour (2015) 7:917–944
Pinus yunnanensis
LG
Species
PyTr10n
PyTr09n
PyTr07n
PyTr06n
PyTr04n
PyTr02n
Mean
PyTr32n
PyTr31n
PyTr30n
PyTr28n
PyTr27n
PyTr26n
PyTr24n
PyTr23n
PyTr22n
PyTr20n
PyTr19n
PyTr17n
F: TGAAAGCGAAGTAGCTTTGGTAA
PyTr15n
R: AAACTGAGAAACTTCTGGAAGGC
F: AATTCTCATTCTCTCCTGCAGAC
R: CAGATTCCATCATAATAGCAGCC
F: AGAGAATTAGCCAGATGATGTGC
R: GAGCTGTTGACACCCATACTAGC
F: ATGTTAGAGCAACACAGGAGAGC
R: ATCACAATATCTTCTGCTGCGTC
F: ATGCTGGTGACATTAAATCCAAG
R: TATCATCTCACTACGAAGGGGTG
F: GGAACCATAAACAATCCAAACAA
R: ACCTTCATCGACAGTGTTGTTCT
F: ATGACTTCTCCAAATGCTCAGTC
R: AGGCCCTTTAATGCACTAGACAC
F: CGATAGTTTAGTTTGGCTGAGGA
R: AGAACAGTCTGGACATCATGGTT
F: AAAAGCTCATCATGCATTCTTTC
R: GAGAGAATGAAGAAGTTCACCCA
F: GGAGAATTCAAACACGAGAAAGA
R: GTTGTATGTTTATGTGCAGCGTT
F: AGAAAAGTTTTGGTTGTGACACG
R: CAAGTCTTTTTACCGTGTAAGGTG
F: GCATTGTGAGGGGTTTCTTAAAT
R: ATATATCCCCACGGTTCTTTACC
F: AAGAACTTGACATTTTGAACCCA
R: CTGGATTCATTTGTGGCTAAGAC
F: CAAAACCCAACGAATTAAGACAG
R: CTCTTACAGGCTGTGGAACCTCT
F: CTAAGTGTGGAAAGTTTGTTGGC
R: AACCAATGTGTAGCGAGTATGGT
F: GGGGTTATCAAAGAACGAGACTT
R: AAAATGTGGCCACTGGTACTAAA
F: CATCTTCATCTTCATCATCATCCT
R: CCAGAGGGGTATCCATAGGTAAG
F: GGGGTTATCAAAGAACGAGACTT
R: TGGAGAGGATTGCTGAGATACTT
F: ACAGCAACATTTAAGTCAGCGTT
R: ACAAGAACCATGATAAATCGCTG
Primer sequence (50 –30 )
Locus
GAA
GAG
TGA
AAG
GGT
ATG
AT
AT
GA
CA
AT
TA
AT
TA
GGA
TCA
GGA
CAG
TCC
Repeat motif
155–158
153–159
147–201
141–147
136–160
100–145
160–166
126–128
159–165
158–162
125–147
126–130
142–156
165–185
156–183
170–176
158–167
149–152
173–176
Size range (bp)
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
Sample size (N)
2
3
5
3
4
5
3.7
4
2
4
3
8
3
4
6
7
3
3
2
2
Number of alleles (NA)
0.167 (0.486)
0.167 (0.292)
0.667 (0.778)
0.333 (0.625)
0.167 (0.597)
0.833 (0.736)
0.532 (0.531)
0.833 (0.653)
0.500 (0.375)
0.500 (0.722)
0.667 (0.486)
0.333 (0.861)
1.000 (0.569)
0.333 (0.597)
0.667 (0.750)
0.833 (0.806)
0.833 (0.569)
0.500 (0.486)
0.167 (0.153)
1.000 (0.500)
Ho (He)
Conservation Genet Resour (2015) 7:917–944 939
123
123
Pinus yunnanensis
YJ
Species
PyTr07n
PyTr06n
PyTr04n
PyTr02n
Mean
PyTr32n
PyTr31n
PyTr30n
PyTr28n
PyTr27n
PyTr26n
PyTr24n
PyTr23n
PyTr22n
PyTr20n
PyTr19n
PyTr17n
PyTr15n
PyTr13n
F: GAGCTTCTATTTCCTTTATCGGC
PyTr12n
R: GAGCTGTTGACACCCATACTAGC
F: ATGTTAGAGCAACACAGGAGAGC
R: ATCACAATATCTTCTGCTGCGTC
F: ATGCTGGTGACATTAAATCCAAG
R: TATCATCTCACTACGAAGGGGTG
F: GGAACCATAAACAATCCAAACAA
R: ACCTTCATCGACAGTGTTGTTCT
F: ATGACTTCTCCAAATGCTCAGTC
R: AGGCCCTTTAATGCACTAGACAC
F: CGATAGTTTAGTTTGGCTGAGGA
R: AGAACAGTCTGGACATCATGGTT
F: AAAAGCTCATCATGCATTCTTTC
R: GAGAGAATGAAGAAGTTCACCCA
F: GGAGAATTCAAACACGAGAAAGA
R: GTTGTATGTTTATGTGCAGCGTT
F: AGAAAAGTTTTGGTTGTGACACG
R: CAAGTCTTTTTACCGTGTAAGGTG
F: GCATTGTGAGGGGTTTCTTAAAT
R: ATATATCCCCACGGTTCTTTACC
F: AAGAACTTGACATTTTGAACCCA
R: CTGGATTCATTTGTGGCTAAGAC
F: CAAAACCCAACGAATTAAGACAG
R: CTCTTACAGGCTGTGGAACCTCT
F: CTAAGTGTGGAAAGTTTGTTGGC
R: AACCAATGTGTAGCGAGTATGGT
F: GGGGTTATCAAAGAACGAGACTT
R: AAAATGTGGCCACTGGTACTAAA
F: CATCTTCATCTTCATCATCATCCT
R: CCAGAGGGGTATCCATAGGTAAG
F: GGGGTTATCAAAGAACGAGACTT
R: TGGAGAGGATTGCTGAGATACTT
F: ACAGCAACATTTAAGTCAGCGTT
R: ACAAGAACCATGATAAATCGCTG
F: TGAAAGCGAAGTAGCTTTGGTAA
R: AGCTAATAGCAGTGAGCTTCTGG
F: TCATACAGTGGATGTTGGAGAAC
R: CTTGAAAGTTGAGGAGTCTTTGC
Primer sequence (50 –30 )
Locus
TGA
AAG
GGT
ATG
AT
AT
GA
CA
AT
TA
AT
TA
GGA
TCA
GGA
CAG
TCC
CTG
TTC
Repeat motif
147–159
141–144
133–154
103–145
160–164
126–128
157–167
156–162
129–141
126–132
142–156
177–183
156–180
170
164–167
143–149
173–176
167–173
162–168
Size range (bp)
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
Sample size (N)
2
2
4
4
3.3
3
2
6
4
3
3
4
4
6
1
2
2
2
3
3
Number of alleles (NA)
0.167 (0.153)
0.667 (0.500)
0.000 (0.667)
1.000 (0.694)
0.468 (0.511)
0.833 (0.625)
0.333 (0.278)
0.500 (0.806)
0.500 (0.514)
0.167 (0.292)
0.500 (0.569)
0.500 (0.708)
0.667 (0.653)
1.000 (0.806)
0.000 (0.000)
0.333 (0.278)
0.167 (0.153)
0.833 (0.486)
1.000 (0.569)
0.167 (0.486)
Ho (He)
940 Conservation Genet Resour (2015) 7:917–944
Acrossocheilus wenchowensis
Species
161–179
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
24
Awc 004o
R: CCTGGAGATACTGAACAACA
F: TCCTTCTCTTCCTCTACTACT
R: GTGTTCTACGGCTATGTCTC (TC)5
180–200
32
32
(CT)12
160–168
126–134
157–165
158–162
131–143
124–128
144–156
179–185
159–168
167–176
167
143–149
173–176
164–173
168
158
24
Sample size (N)
24 F: TGTTATGTTCAGTGACTCCTC
AT
AT
GA
CA
AT
TA
AT
TA
GGA
TCA
GGA
CAG
TCC
CTG
TTC
GAA
156–159
Size range (bp)
Awc 002o
R: AGGCCCTTTAATGCACTAGACAC
F: CGATAGTTTAGTTTGGCTGAGGA
R: AGAACAGTCTGGACATCATGGTT
F: AAAAGCTCATCATGCATTCTTTC
R: GAGAGAATGAAGAAGTTCACCCA
F: GGAGAATTCAAACACGAGAAAGA
R: GTTGTATGTTTATGTGCAGCGTT
F: AGAAAAGTTTTGGTTGTGACACG
R: CAAGTCTTTTTACCGTGTAAGGTG
F: GCATTGTGAGGGGTTTCTTAAAT
R: ATATATCCCCACGGTTCTTTACC
F: AAGAACTTGACATTTTGAACCCA
R: CTGGATTCATTTGTGGCTAAGAC
F: CAAAACCCAACGAATTAAGACAG
R: CTCTTACAGGCTGTGGAACCTCT
F: CTAAGTGTGGAAAGTTTGTTGGC
R: AACCAATGTGTAGCGAGTATGGT
F: GGGGTTATCAAAGAACGAGACTT
R: AAAATGTGGCCACTGGTACTAAA
F: CATCTTCATCTTCATCATCATCCT
R: CCAGAGGGGTATCCATAGGTAAG
F: GGGGTTATCAAAGAACGAGACTT
R: TGGAGAGGATTGCTGAGATACTT
F: ACAGCAACATTTAAGTCAGCGTT
R: ACAAGAACCATGATAAATCGCTG
F: TGAAAGCGAAGTAGCTTTGGTAA
R: AGCTAATAGCAGTGAGCTTCTGG
F: TCATACAGTGGATGTTGGAGAAC
R: CTTGAAAGTTGAGGAGTCTTTGC
F: GAGCTTCTATTTCCTTTATCGGC
R: AAACTGAGAAACTTCTGGAAGGC
F: AATTCTCATTCTCTCCTGCAGAC
GAG
Repeat motif
Mean
PyTr32n
PyTr31n
PyTr30n
PyTr28n
PyTr27n
PyTr26n
PyTr24n
PyTr23n
PyTr22n
PyTr20n
PyTr19n
PyTr17n
PyTr15n
PyTr13n
PyTr12n
PyTr10n
F: AGAGAATTAGCCAGATGATGTGC
PyTr09n R: CAGATTCCATCATAATAGCAGCC
Primer sequence (50 –30 )
Locus
3
7
3.0
5
5
5
3
3
3
4
4
3
3
1
3
2
4
1
1
2
Number of alleles (NA)
0.094 (0.417)
0.469 (0.731)
0.468 (0.438)
0.833 (0.667)
0.833 (0.750)
0.500 (0.792)
0.500 (0.403)
0.333 (0.500)
0.667 (0.611)
0.667 (0.681)
1.000 (0.708)
0.333 (0.292)
0.333 (0.292)
0.000 (0.000)
0.333 (0.292)
0.667 (0.444)
0.833 (0.597)
0.000 (0.000)
0.000 (0.000)
0.167 (0.153)
Ho (He)
Conservation Genet Resour (2015) 7:917–944 941
123
Species
123
Awc 085o
Awc 080o
Awc 071*,o
Awc 070*,o
Awc 069o
Awc 063o
Awc 059o
Awc 051*,o
Awc 046o
Awc 043b*,o
Awc 043a
Awc 042o
Awc 041*,o
Awc 019o
Awc 017o
Awc 014*,o
Awc 011o
Awc 010o
Awc 009o
F: CTGTTGTAAGGTTGCTGTAG
Awc 005o
R: ACACAATAATCCGACAGAGA
F: CAGATTGAATTTAGCGACAC
R: GTCAGTAGTATCCACTCACAT
F: AGAACAAGAGACAGCAATCA
R: GGTAAGACAGATGAGTGGAA
F: GGTGCTTCATTACTCCTCTC
R: ATCCCTCTTTGTGACTTTCT
F: TCTTTCTGCTGTGTTTCTTC
R: CTCAACAGAGACAATACATCAG
F: TAATCCAGTAAGGCACCAGA
R: AGTCAATGGATCTCTCTGTAG
F: CTCTTCTGTCTTCACCTCTC
R: TTTACATTCTCTCAGCCTGT
F: CCAAAGACACAACCACAAAG
R: CATGACAGATAACAGGTGAT
F: CAGAATGAATGAAGGAGACA
R: AGATACTGATGGAGGAAGTG
F: TCTCTTGTTCTTCTCTGTCT
R: AGAGGACACAGAAACAGAAT
F: ATGCTCTCCATCATCACATT
R: TGCTGATGTGATCTGATGAG
F: TGTTGCTGACTTGTATGGAA
R: GTGATGATGATTCAGCCTAAG
F: AGCAGGTATCTAGTGTTACG
R: AGAATCAGAATCCGTTTGAG
F: CACACTCCAAACATACTCAA
R: TTACTGACGAGATGCTCATG
F: CAACCAACAAACACAATACC
R: GATACAATAACGGACAGACA
F: CATGACAGATAACAGGTGAT
R: TCAGAGGTCAGATGCAATAG
F: GCCTAGTATGTGTATGTATGTG
R: TTACTGACGAGATGCTCATG
F: ACAAACCAAACCAATGAAGG
R: TCAGACACAGTTCAGATACA
F: CTCCTTTCTCTCTTTCTCTCT
R: GAGAAAGAAGCAAGGACAAA
F: CTTCCCTATTACTTCCCTACTA
R: GACTGATAGACTGATAGACTGA
Primer sequence (50 –30 )
Locus
(TC)7
(TG)21
(TC)14
(AC)10T(CA)17
(AG)9
(TC)7
(AG)6AA(AG)7
(CA)17
(TC)5
(TC)23
(GT)11(AT)2(TG)5
(TC)21
(AACA)5
(AAGA)6
(GT)11
(AG)6
(AAGA)10
(TC)5G(CT)5
(CTTC)6
(GT)5…(TC)6
Repeat motif
274–278
309–351
281–311
129–203
354–360
400–404
92–128
172–200
111–139
173–209
145–175
309–337
195–263
114–144
151–193
361–441
342–344
292–368
302–330
334–366
Size range (bp)
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
32
Sample size (N)
3
18
12
25
4
3
17
7
13
17
15
5
14
14
10
15
2
10
2
14
Number of alleles (NA)
0.214 (0.356)
0.719 (0.928)
0.719 (0.865)
0.594 (0.954)
0.281 (0.307)
0.438 (0.469)
0.839 (0.891)
0.469 (0.801)
0.906 (0.895)
0.469 (0.904)
0.844 (0.902)
0.469 (0.542)
0.321 (0.843)
0.875 (0.920)
0.719 (0.663)
0.423 (0.836)
0.000 (0.062)
0.813 (0.842)
0.219 (0.246)
0.719 (0.908)
Ho (He)
942 Conservation Genet Resour (2015) 7:917–944
XL130p
XL126p
XL124p
XL122p
XL118p
XL114p
XL106p
XL105p
XL71p
XL70p
XL68p
XL66p
XL65p
XL34p
XL31p
XL25p
XL22p
XL21p
XL10p
F: AAACTTGCATCCTCTAAATTGCTT
XL9p
Pyrus betulaefolia Bunge
R: GCGGCTACTATCTCTGAGGC
F: CCGGACAAGGATATTTCCAG
R: TCGAATTATCGATGGTCGGT
F: CTCCCGTAAGTATGGCTGGT
R: TGAAATAATCACTTGACATTTCCTTT
F: CTTCTTAAGGTGATGCTCCAAA
R: TAACTGTCTGTTTGAAGCAAACTT
F: GGAGTGCCAATAACTCTTCCC
R: ACTCCGATCCTTCTTCCCTT
F: CAAGCGCCCTAGATATTAGTTT
R: TTCTCAACAGCCAAGCACTACT
F: CCAGGGCTATGAGTGTTCC
R: GCTGTAGTTTCATACAGTTTCACTTG
F: TTCATTCTAACTCTGCCTTGAA
R: AAGCACCAGAACAATGGAGG
F: CACCACTGAGCTTCCTCTCC
R: CCCACTATCTTCCTAGAAGCACC
F: CAGCTGAGATTATAGAACTGGTTACTT
R: GAAAGGCTTGAAACCGCC
F: AATTTAGCAGTTTCGCTCATATTT
R: AGAGGACGATCACCAGCTTC
F: AAAGTTCAAATGCTCCTCCG
R: CAGGGAGCAAGATAGATCCAA
F: GGTCCAAAGAAGGAGAGGA
R: TCGATGTCATACAACATGGATT
F: TTGAAACTTGGATCTGGATGA
R: TCTTACAAATGTTGTGCTTAGCC
F: AGGGTAGCCAGAGTAGGCG
R: GCGAGGGAGGAGATCAGAG
F: CAACTTTCCAAATCCAAGGG
R: AATCGCTGCAGTGACACATC
F: CAGTAGCTTGTGCGTGCTGT
R: GTGACATTCAATGAGCCGAA
F: CAGCTGCATGGGATAGAATG
R: CGCGTAATGACGTGACAGAT
F: CACCCAACCATAAAGCCTG
R: TGCGCTCGTTAGACCATTT
F: CTGCTGGCATGAAATTCAAA
R: GCTGGTATGAGCTAGGGTCG
Primer sequence (50 –30 )
Locus
Species
AG
AT
AT
AG
AT
AG
AG
AG
AG
AG
AG
AG
AC
AT
AG
AC
AG
AG
AT
AT
Repeat motif
87–91
87–91
121–125
89–129
98–100
106–132
114–118
110–144
108–112
122–130
109–127
94–130
106–110
88–106
90–108
107–127
98–102
95–99
97–101
96–124
Size range (bp)
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
Sample size (N)
3
3
3
6
2
11
3
5
4
4
8
7
3
3
3
9
3
3
3
3
Number of alleles (NA)
0.500 (0.633)
0.500 (0.625)
0.625 (0.664)
0.500 (0.797)
0.375 (0.492)
0.750 (0.875)
0.625 (0.664)
0.625 (0.766)
0.750 (0.664)
0.375 (0.711)
0.750 (0.836)
0.625 (0.805)
0.125 (0.570)
0.375 (0.586)
0.625 (0.617)
0.750 (0.844)
0.500 (0.648)
0.625 (0.625)
0.500 (0.648)
0.375 (0.633)
Ho (He)
Conservation Genet Resour (2015) 7:917–944 943
123
123 R: TTTCGCAATCACATATTGCAC
F: AGAACTTACGCAATCTTGATGTT
R: AACTTTGTAATTGAAACTGTTCATTCT
F: ATGTGGCTATCCGACAGTGC
R: TGCATGCTTAAGATACCAACTAAA
F: TTAATCGTTGTTTATCTACTGTCGAG
R: AGAATCCTCTCTGGATCATTTCC
F: AAATCAATGACCCATGATGTGA
AC
AT
AT
AG
AT
Repeat motif
114–118
89–93
116–120
88–120
88–94
Size range (bp)
Corresponding to Acr1-60 (Tang et al. 2010), mentioned because the product size is different for designing new panels
Cmax15 was homologous to Isurus oxyrinchus (shortfin mako shark), microsatellite CA1XD
#
high frequency of null alleles (19.5%), 9failed amplification
* Significant deviation from Hardy -Weinberg equilibrium (*p \ 0.05, **p \ 0.01, ***p \ 0.001, ****p \ 0.0021) indicates locus may harbor null alleles (null allele frequency [ 5%);
8
8
8
8
8
Sample size (N)
Same microsatellite locus isolated from two separate Illumina paired-end libraries: the repeat enriched BSUK05_17 and unenriched BSUK05_35
Microsatellite loci sequenced using MiSeq from enriched individual BSUK05_17
Microsatellite loci sequenced using Sanger from enriched individual BSUK05_17
J. Tian, B. Zeng, M. Liu, S. Luo, X. Li, J. Li (ESM 16)
Y.Y. Jia, S.L. Liu, X.L. Li, Z.M. Gu, J.L. Zhao, W.P. Jiang, Q.P. Lian, J.L. Guo (ESM 15)
N. Cai, Y. Xu, Y. Xu, D. Wang, B. Tian, C. He, A. Duan (ESM 14)
R.M. Martin, M.L. Robinson, W.D. Wilson (ESM 13)
M. Ruhsam, A.S. Wulff, B. Fogliani, P.M. Hollingsworth (ESM 12)
Y. Sato, H. Ito, M. Onuma, T. Maeda, R. Ogden, M. Inoue-Murayama (ESM 11)
L. Liu, T. Yanagimoto, C.H. Li, N. Song, T.X. Gao (ESM 10)
G. Zhang, J. Chen, S. Yin, P. Tao, R. Wang, X. Wang, Y. Ding, X. Wang (ESM 9)
R.K. Basiita, K.R. Zenger, D.B. Jones, D.R. Jerry (ESM 7) P. Ge´lin, C. Rougeux, V. Mehn, M.M.M. Guillaume, J.H. Bruggemann, H. Magalon (ESM 8)
C. Chen, Q. Li, H. Yu, L. Kong (ESM 6)
L. Lieber, D.A. Dawson, G.J. Horsburgh, L.R. Noble, C.S. Jones (ESM 5)
H. Sungani, M.J. Genner (ESM 3) V. Noguerales, C. Ca´liz-Campal, P.J. Cordero, J. Ortego (ESM 4)
A. Oleksa, K. Meyza, L. Cizek, L. Drag (ESM 2)
X. Wu, M. Wu, X. Su, Y. Zhou, Y. Pan (ESM 1)
XL143p
XL141p
XL139p
XL138p
F: ATTAGCCATTGACGGGATTC
XL135p R: CAGTATCTCTTGGAGAAGAATTATTTG
Primer sequence (50 –30 )
Locus
N sample size; NA number of alleles; HO, observed heterozygosity; HE, expected heterozygosity
5
4
3
2
1
p
o
n
m
l
k
j
i
h
g
f
e
d
c
b
a
Species
3
3
3
5
3
Number of alleles (NA)
0.750 (0.625)
0.500 (0.648)
0.500 (0.586)
0.625 (0.773)
0.000 (0.594)
Ho (He)
944 Conservation Genet Resour (2015) 7:917–944
