The Plant Journal (2008) 54, 582–592

doi: 10.1111/j.1365-313X.2008.03480.x


Microbial conversion of sugars from plant biomass to lactic acid or ethanol Joy Doran-Peterson*, Dana M. Cook and Sarah K. Brandon Microbiology Department, 1000 Cedar Street, 527 Biological Sciences Building, University of Georgia, Athens, GA 30602, USA Received 13 November 2007; revised 20 February 2008; accepted 21 February 2008. * For correspondence (fax +1 706 542 2674; e-mail [email protected]).

Summary Concerns for our environment and unease with our dependence on foreign oil have renewed interest in converting plant biomass into fuels and ‘green’ chemicals. The volume of plant matter available makes lignocellulose conversion desirable, although no single isolated organism has been shown to depolymerize lignocellulose and efficiently metabolize the resulting sugars into a specific product. This work reviews selected chemicals and fuels that can be produced from microbial fermentation of plant-derived cell-wall sugars and directed engineering for improvement of microbial biocatalysts. Lactic acid and ethanol production are highlighted, with a focus on engineered Escherichia coli. Keywords: plant sugars, ethanol, lactic acid, Escherichia coli, hemicellulose, pectin.

Introduction Plant biomass served as the fuel throughout history until the recent dominance of petroleum during the last century. Concern for our environment and unease with our dependence on imported oil have revived interest in plant-derived fuels and chemicals. Fuel production from locally grown plant material supports CO2 neutral energy production, together with the promise of recovery for economically disadvantaged rural areas (Antoni et al., 2007). Lignocellulosic biomass, such as wood, grasses and agricultural residues, is the most abundant fermentation substrate that can be grown in non-agricultural, marginal lands for conversion to fuels and chemicals. Microbial conversion of plant biomass-derived sugars encompasses a variety of processes using various micro-organisms and enzymes to produce ethanol, lactate, butanol, acetone, acetate, succinate, and other products. Starch and sucrose biosynthesis and uses, cell-wall carbohydrates, and their modification for pulp production, and modification of lignin are reviewed elsewhere in this issue (see Li et al., 2008; Pauly and Keegstra, 2008; Smith, 2008, respectively). This paper focuses on fermentation of plant-derived sugars to produce lactate and ethanol using native and engineered micro-organisms. Fermentation processes that include conversion of sugars from lignocellulose offer great potential for expanding the 582

production capacity for renewable products. Current industrial ethanol production uses sugar cane molasses or starch fermented by yeast to produce ethanol at concentrations of up to 20% v/v (Antoni et al., 2007). Although glucose resulting from starch breakdown is readily fermented by a number of micro-organisms to ethanol, butanol and other products, a dramatic increase in starch-based fermentations is impractical because of competition for limited prime agricultural lands used for food and feed production to support an ever-increasing population. However, a significant proportion of marginal lands that are not suitable for intense agriculture for food production can be used to produce lignocellulosic biomass for bioconversion to fuels and chemicals. Plant biomass is a mixture of carbohydrate polymers (cellulose, hemicellulose and pectin, to varying degrees) and the non-carbohydrate polymer lignin. Cellulose consists of long microfibrils containing repeating units of cellobiose, which are dimers of glucose molecules. These hydrogen-bonded microfibrils may be quite long; up to 14 000 glucose units have been observed in Arabidopsis, corresponding to a fibril length of 7 lm (Sommerville et al., 2004). Hemicelluloses are branched polysaccarides with backbones of neutral sugars hydrogen-bonded to cellulose. Pectins are the cement that holds the plant cell walls ª 2008 The Authors Journal compilation ª 2008 Blackwell Publishing Ltd

Microbial conversion of sugars from plant biomass 583 together and are defined by the presence of uronic acids. Homogalacturonan (HG) is an unbranched polymer of (1 fi 4)a-D-galacturonic acid. Another pectic material, rhamnogalacturonan I (RGI), comprises a backbone of alternating (1 fi 2)a-L-rhamnose–(1 fi 4)a-D-galacturonic acid containing mainly arabinan and galactan side chains (Rose et al., 2000). RGI apparently functions as a scaffolding to which other pectins are attached (Vincken et al., 2003). Table 1 shows the composition of selected plant biomass substrates and the resulting sugars generated by depolymerization. Although starch can be easily depolymerized to glucose units using two enzymes, extraction of soluble sugars for fermentation from lignocellulosic biomass is more complex due to the diversity and structural integrity of lignocellulose. The major sugars found in lignocellulose are shown in Figure 1. A more detailed description of their complex linkages, including lignin cross-linking, is beyond the scope of this paper but is discussed elsewhere in this issue (Pauly and Keegstra, 2008). Sommerville et al. (2004) also provide a more in-depth discussion of plant cell-wall linkages. Factors affecting the hydrolysis of lignocellulose include the accessible surface area of the biomass, cellulose fibre crystallinity, and lignin and hemicellulose content (McMillan, 1994a). Deconstruction of these integrated plant polymers may include thermochemical pre-treatment, such as heating or treating with an acid or base, and/or mechanical pre-treatment to allow enzymatic access to convert the polymers to fermentable sugars. Agricultural residues that are already collected and partially processed can sometimes circumvent the additional pre-treatment. A number of excellent reviews have described various lignocellulose pre-treatment protocols used to enhance enzyme accessibility for plant cell-wall deconstruction (Kenealy et al., 2007; McMillan, 1994a; Mosier et al., 2005). It is worth mentioning that other compounds are generated as by-products of pretreatment regimes, and some of these compounds may be inhibitory to both the fermenting organism and the enzymes

used for breaking polymers down to dimers or monomers (Almeida et al., 2007; Delgenes et al., 1996; Mussatto and Roberto, 2004). Once the soluble sugars are produced, a number of naturally occurring biocatalysts are able to ferment the sugars to lactic acid (species in the genera Lactobacillus, Lactococcus, Streptococcus, Pediococcus, and others), ethanol (Saccharomyces cerevisiae, Zymomonas mobilis, Pichia stipitis) and butanol (Clostridium acetobutylicum, Clostridium beijerinckii), among others (Bothast and Schlicher, 2005; Drake and Daniel, 2004; Hofvendahl and Hans-Hagerdal, 2000; Huang et al., 1998; Jones and Woods, 1986). Industrial-scale production of lactic acid, ethanol and butanol has been achieved by microbial fermentation of sugars (Bothast and Schlicher, 2005; Hofvendahl and Hans-Hagerdal, 2000). Lactic acid and ethanol are produced as the sole product of fermentation by naturally occurring organisms at high yields due to their redox neutral state (Bothast and Schlicher, 2005; Hofvendahl and Hans-Hagerdal, 2000). Industrial ethanol fermentations in the USA use the yeast S. cerevisiae to convert glucose derived from corn starch into ethanol and CO2 using the Embden–Meyerhof–Parnas pathway. Although Z. mobilis, an anaerobic bacterium, also metabolizes glucose to ethanol and CO2 using a different pathway (Entner–Doudoroff) with significantly higher specific productivity than yeast, yeast-based fermentation dominates the industry. Lactic acid production from sugars or glucose is mainly achieved by fermentation with lactic acid bacteria. This review focuses on the development of biocatalysts for conversion of hexoses and pentoses derived from lignocellulosic biomass. Other reviews have focused on the microbial production of various other commodity chemicals, such as succinate, acetate and butanol from biomass (Datta and Henry, 2006; Hahn-Ha¨gerdal et al., 2006; Lynd et al., 2005; McKinlay et al., 2007; Narayanan et al., 2004; Ragauskas et al., 2006; Singh et al., 2006; Wyman, 2003).

Table 1 Selected lignocellulosic biomass compositions (% dry weight) Grass

Feedstock Cellulose Hemicellulose Xylan 5C Arabinan 5C Mannan 6C Galactan 6C Lignin Pectin

Agricultural residues

Hardwood (hybrid poplar)a

Softwood (pine)a



Sugar beet pulpc

Wheat strawd

Corn stovere

44.7 18.6 14.6 0.8 2.2 1.0 26.4

44.6 21.9 6.3 1.6 11.4 2.6 27.7 ND

32.0 25.2 21.1 2.8 0.3 1.0 18.1 ND

32.4 25.1 19.4 4.6 ND 1.1 20.3 ND

24.0 29.2 2.0 21.0 1.1 5.1 2.0 24.0

45.0 25.7 20.0 3.5 0.0 2.2 18.0 ND

37.4 27.6 21.1 2.9 1.6 2.0 20.4 1.1


Wyman (1996); bSun and Cheng (2005); cDoran et al. (2000); dLequart et al. (2000); eSheehan et al. (2004). ND, not determined.

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584 Joy Doran-Peterson et al.







D-xylose D-glucose



D- mannose


Figure 1. Structures of plant biomass sugars. (a) Cellulose is composed of repeating units of cellobiose (dimers of glucose), with the monomeric form being glucose. (b) Two examples of hemicellulose structures: (1) xyloglucan polymer with monomeric forms of glucose and xylose, and (2) galactomannan with monomeric forms of galactose and mannose.

Lactic acid (CH3CHOHCOOH; C3H6O3) Naturally occurring lactic acid bacteria (LAB) ferment hexose sugars, such as glucose, by oxidizing NADH generated during glycolysis, with pyruvate serving as the electron acceptor, to form lactic acid as the major product (Figure 2). These organisms have been used to produce lactic acid for food preservation, food packaging, drug delivery compounds, medical implants, cosmetic ingredients, and biodegradable plastic (Agrawal and Bhalla, 2003; Lee et al., 2004; Ray and Bousmina, 2005). Lactic acid exists in two enantiomeric forms, L-(+) and D-()), and the ratio of the two isomeric forms influence the physicochemical properties of the polylactide polymer (PLA; Narayanan et al., 2004). LAB usually possess two genes that encode lactate dehydrogenase enzymes (LDH), which catalyse formation of either L-(+) or D-()) lactic acid (Hofvendahl and Hans-Hagerdal, 2000; Makarova et al., 2006). The ratio of the L-(+) to D-()) forms of lactic acid in the fermentation broth depends on the level of expression of these two genes. Although all LAB ferment glucose and other hexoses, their ability to ferment pentoses (xylose, arabinose, etc.) is generally limited. Lactococcus lactis and Lactococcus casei, as well as

Lactobacillus plantarum and Lactobacillus buchneri, are among those LAB with this capability (Table 2). L. lactis utilizes heterofermentative metabolism when metabolizing pentoses (Figure 2b), with production of an equal mixture of lactate and acetate (Bolotin et al., 2001; Cocaign-Bousquet et al., 1996). Although LAB have been used for the production of lactic acid on an industrial scale for decades, their inability to ferment a broad range of sugars and requirements for complex media have hampered the use of PLA as a renewable plastic. This is more critical as we attempt to replace petroleum-based plastics with renewable PLA-based plastics, which would require production of the base chemical in large quantities and lignocellulosic biomass as the feedstock for fermentation. Biocatalysts currently being developed to reduce the cost of production of optically pure isomers of lactic acid include Kluyveromyces (Bianchi et al., 2001; Porro et al., 1999), Saccharomyces (van Maris et al., 2004; Saitoh et al., 2005), Pichia (Ilmen et al., 2007), Rhizopus (Skory, 2000) and Lactobacillus (Liu et al., 2007); however, Escherichia coli stands out as an excellent microbial biocatalyst for this purpose (Dien et al., 2001; Grabar et al., 2006).

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Microbial conversion of sugars from plant biomass 585



Figure 2. Heterofermentative pathways of lactic acid production using glucose (a) or xylose (b) as substrate. (a) Glucose fermentation. 1, glucose transport; 2, phosphohexose isomerase and phosphofructokinase; 3, aldolase; 4, glyceraldehyde-3-phosphate dehydrogenase; 5, phosphoglycerate kinase; 6, phosphoglyceromutase; 7, enolase; 8, pyruvate kinase; 9, lactate dehydrogenase; 10, pyruvate formate lyase; 11, phosphotransacetylase and acetate kinase; 12, alcohol dehydrogenase; 13, methylglyoxal synthase; 14, enzymes of the methyl glyoxal pathway to lactate; 15, phosphotriose isomerase. DHAP, dihydroxyacetone phosphate; GAP, glyceraldehyde-3-phosphate; PGA, phosphoglyceric acid; PEP, phosphoenolpyruvate. (b) Xylose fermentation. 1, xylose isomerase; 2, xylulokinase; 3, phosphoketolase; 4, acetate kinase; 5, phospohotransacetylase; 6, aldehyde dehydrogenase; 7, alcohol dehydrogenase; 8, transketolase; 9, multiple enzymes involved in conversion of glyceraldehyde-3-phosphate to pyruvate; 10, pyruvate formate lyase; 11, lactate dehydrogenase.

Table 2 Lactic acid production from glucose (G) or xylose (X) using yeast, fungi or bacteria Organisms


Total lactate (g l)1)

Yield (%)


Lactobacillus sp. (NRRL B-30574) Lactobacillus helveticus (GRL89) Lactobacillus delbrueckii (mutant DP3) Lactococcus lactis Lactococcus lactis (10-1 JCM 7638) Lactococcus casei subsp. rhamnosus (ATCC 10863) Lactobacillus buchneri (NRRL B-30929) Kluyveromyces marxianus (CD590) Kluyveromyces lactis (BM-12D, pLAZ10) Kluyveromyces lactis (PMI/C1, pEPL2) Saccharomyces cerevisiae (RWB876) Saccharomyces cerevisiae (OC2T T165R) Pichia stipitis Escherichia coli (TG114) Escherichia coli (FBR9, FBR11) Escherichia coli (FBR11 pVALDH1) Escherichia coli (SZ79) Escherichia coli (SZ85)

G G G G X X X G G G G G X G X G G G, X

103 75 117 32 33 25 40, 83 (fed batch) 81 60 109 61 122 58 118 56–63 73 4.3 4.5, 3.95

79 92 56 63 60 80 60 81 55 55 82 61 58 98 78 73 86 94, 82

Eddington et al. (2004) Kyla-Nikkila et al. (2000) Demirci and Pometto (1992) Cock and de Stouvenel (2006) Tanaka et al. (2002) Iyer et al. (2000) Liu et al. (2007) Rajgarhia et al. (2004) Bianchi et al. (2001) Porro et al. (1999) Liu and Lievense (2005) Saitoh et al. (2005) Ilmen et al. (2007) Grabar et al. (2006) Dien et al. (2001) Dien et al. (2001) Zhou et al. (2003b) Zhou et al. (2003b)

E. coli is a well studied bacterium that uses glycolysis via the Embden–Meyerhoff–Parnas (EMP) pathway to convert hexose sugars into a mixture of acids (lactic, acetic, formic and succinic) and ethanol (Clark, 1989). E. coli also metabolizes pentose sugars through the pentose phosphate pathway, which feeds intermediates into the EMP pathway

towards homolactate production, in contrast to the use of phosphoketolase by lactic acid bacteria, which leads to production of a mixture of lactic acid and acetic acid during pentose fermentation. Because E. coli ferments all the sugars present in plant biomass in a simple salt-based medium, this organism has been engineered for production

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586 Joy Doran-Peterson et al. of optically pure lactic acid by deleting competing pathways (Chang et al., 1999; Dien et al., 2001; Zhou et al., 2003a,b, 2005). E. coli produces only the D-())-LDH enzyme, and the resulting product is 100% D-()) lactic acid (Zhou et al., 2003a). Zhou et al. (2003b) further engineered E. coli by deleting the native d-ldh gene and replacing it with the gene encoding L-(+)-LDH from Pediococcus to produce optically pure L-(+)-lactic acid. Further metabolic engineering and directed evolution led to E. coli strains, TG114 and TG108, that produced optically pure (99.9%) D-()) and L-(+)-lactate, respectively, from 12% w/v glucose at greater than 95% of the theoretical yield (Grabar et al., 2006). The very high lactic acid production titre and yield achieved by engineered E. coli strains TG114 and TG108 in simple salts medium is equal to or higher than the best values reported for lactic acid bacteria in complex medium (Table 2). Apparently, these E. coli derivatives have overcome some of the growthand fermentation-inhibitory properties of high concentrations of lactic acid (Pieterse et al., 2005). The engineered lactic acid-producing E. coli demonstrate the power of directed evolution of the fermenting organism coupled with metabolic pathway engineering for production of desired product (Grabar et al., 2006). Ethanol (CH3CH2OH; C2H6O) Saccharomyces yeast is the main biocatalyst for commercial ethanol production from sugar cane or corn starch. Yeast fermentation of corn involves a liquefaction step for starch solubilization, hydrolysis to break the polymer down to glucose, followed by fermentation of the glucose to ethanol. In contrast to starch processes, plant biomass processes include a mixture of five-carbon (C5) and six-carbon (C6) sugars and inhibitors released from the plant itself (i.e. ferulic acid, organic acids) and/or generated during pre-treatment (i.e. furfural, hydroxymethylfurfural; Larsson et al., 2000; McMillan, 1994b; Zaldivar and Ingram, 1999). Depending upon the type of plant biomass, chemical pre-treatment and enzymes used, much of the potentially fermentable sugar content may be pentoses. However, Saccharomyces and the bacterium Z. mobilis, which are naturally ethanologenic microbial biocatalysts, lack the native ability to ferment pentose sugars, the major component of the hemicellulose fraction of plant biomass (Feldmann et al., 1992; HahnHa¨gerdal et al., 1993). There are naturally occurring xylose-fermenting, ethanol-producing microbes; however, their use is limited due to inhibition by compounds generated during plant biomass pre-treatment, by-product formation or slow rates of ethanol production (HahnHa¨gerdal et al., 2006). To meet the challenge of fermenting all the plant biomass sugars to ethanol, a number of engineered bacteria and yeasts have been produced. Pentose-fermenting S. cerevisiae strains have been

generated by adding the xylose metabolic pathway from P. stipitis (Kotter and Ciriacy, 1993) and introducing the xylose isomerase gene either from a bacterium (Thermus thermophilus; Walfridsson et al., 1996) or from an anaerobic fungus (Piromyces sp.; Kuyper et al., 2003; van Maris et al., 2007). Arabinose utilization capability has been introduced by adding fungal (Richard et al., 2003) and bacterial (Sedlak and Ho, 2001) arabinose-metabolizing genes. Pentose fermentation in industrial strains of S. cerevisiae such as TMB 3006, TMB 3400 and 424A (LNF-ST), containing heterologous xylose reductase and xylitol dehydrogenase, achieved yields of over 0.4 g ethanol per g sugar (Hahn-Ha¨gerdal et al., 2007). S. cerevisiae metabolic engineering is still a topic of great interest, and a number of excellent reviews have addressed this specific topic (Ho et al., 2000; Jeffries and Jin, 2004; Ostergaard et al., 2000). Metabolic pathway engineering may also improve the ethanol production of some native xylose-metabolizing yeasts such as P. stipitis, Pichia segobiensis, Candida sheatae and Pachysolen tannophilus, although the metabolic potential of these yeasts is still poorly understood (Jeffries, 2006). The bacterium Z. mobilis also efficiently produces ethanol from glucose and tolerates up to 12% w/v ethanol (Dien et al., 2003), but does not produce ethanol from pentose sugars. A xylose-fermenting Z. mobilis was engineered by introducing a xylose-metabolizing pathway from E. coli (Zhang et al., 1995); however, the ethanol tolerance appears to be significantly lower when fermenting a xylose/glucose mixture compared to glucose alone (Joachimsthal and Rogers, 2000). More recently, a xylose- and arabinose-fermenting strain of Z. mobilis was produced (Mohagheghi et al., 2002). This strain, AX101, used glucose, then xylose and finally arabinose in mixed-sugar fermentations, and was hampered by acetic acid toxicity. Further exploration of these engineered Z. mobilis biocatalysts is still of interest due to their ability to ferment at low pH and form minimal by-products (Mohagheghi et al., 2002). Although E. coli lacks the native ability to produce ethanol as the major fermentation product, it uses both hexose and pentose sugars (Lin and Tanaka, 2006) and the uronic acid constituents of pectin (Grohmann et al., 1994). Engineering of the Z. mobilis ethanol pathway in E. coli has been previously reviewed (Dien et al., 2003; Ingram et al., 1997, 1999; Lin and Tanaka, 2006; Zaldivar et al., 2001). Briefly, pyruvate decarboxylase (PDC) and alcohol dehydrogenase II (ADH II) genes from Z. mobilis were integrated into the chromosome of E. coli to generate E. coli strain K011. The Z. mobilis PDC has a low Km for pyruvate compared with other pyruvate-consuming reactions in the cell, such as lactate dehydrogenase and pyruvate formate lyase, effectively shifting metabolic products to higher concentrations of ethanol (Figure 3).

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Microbial conversion of sugars from plant biomass 587

Figure 3. Conversion of hexose and pentose sugars to ethanol by recombinant E. coli in conjunction with the Z. mobilis ethanol pathway. Native E. coli reactions are indicated by a solid arrow; those from Z. mobilis with a dashed arrow.

The easily manipulated genetic system, broad substrate range, and history of industrial use made E. coli a good choice for engineering of a microbial biocatalyst for a lignocellulose-to-ethanol process. A brief summary of the various ethanologenic E. coli derivatives is presented below. Fermentations using E. coli strains KO11 and LY01 E. coli strain KO11 and derivatives have been used for laboratory production of ethanol from many types of plant biomass including rice hulls (Moniruzzaman and Ingram, 1998), sugar cane bagasse, agricultural residues (Asghari et al., 1996), corn cobs, hulls and ammonia fiber explosion (AFEX)-pre-treated fibres (Beall et al., 1992; Lau et al., 2007; Moniruzzaman et al., 1996), orange peel (Grohmann et al., 1994), pectin-rich beet pulp (Doran et al., 2000), sweet whey (Leite et al., 2000), brewery

waste (Rao et al., 2007), cotton gin waste (Jeoh and Agblevor, 2001) and waste house wood (Okuda et al., 2007), in addition to several other sources. Ethanol production and yield on a grams of total ethanol per gram of sugar basis is presented in Table 3. KO11 was recently used in a 1000-l scale bioethanol process using dilute acid hydrolysis of waste house wood, in which all of the xylose was used and a yield of 0.45 g ethanol per g sugar for a 63 h fermentation was obtained (Okuda et al., 2007). Many biomass substrates are subjected to dilute acid hydrolysis to release the pentose sugars and make the cellulose amenable to cellulases, although fermentation inhibitors are created during the process (Dupreez, 1994; McMillan, 1994b). Furfural and 5-hydroxymethylfurfural are pentose and hexose sugar derivatives, respectively, and can inhibit microbial growth (Beall et al., 1991; Zaldivar et al., 1999). Boopathy et al. (1993) demonstrated biotransformation of furfural and 5-hydroxymethylfurfural by enteric bacteria. Ethanologenic E. coli KO11 has been shown to have the ability to transform furfural to less toxic furfuryl alcohol (Gutierrez et al., 2002), demonstrating the potential of E. coli to function even in the presence of some of the inhibitors generated during pre-treatment of biomass. An E. coli K011 derivative, strain LY01, is more resistant than many ethanologens to naturally occurring plant biomass-derived inhibitors and inhibitors generated during chemical pre-treatments (Zaldivar et al., 1999). E. coli LYO1 was used to ferment pressurized-batch hot water-hydrolysed bermudagrass (Brandon et al., 2007). Bermudagrass contains lignified cell walls and low-molecular-weight phenolic acids ester-linked to arabinose (Hartley and Ford, 1989). Hot water pre-treatment can release acetyl groups from hemicellulose, increase depolymerization, and reduce pH (Hamelinck et al., 2005). An ethanol yield of 0.40 g ethanol per g sugar (0.2 g ethanol per g dry weight total

Table 3 Selected plant biomass types and ethanol yield for ethanologenic enteric micro-organisms Biomass


Biomass pre-treatment

Yield (g TE g S)1)a


Corn hulls Pinus sp. (softwood) Crystalline cellulose Sugar cane bagasse Orange peel Mixed waste office paper Corn hulls and fibres Corn stover Sugar cane bagasse Rice hulls Beet pulp Beet pulp Sweet whey Bermudagrass Waste house wood

E. coli KO11 E. coli KO11 K. oxytoca P2 K. oxytoca P2 E. coli KO11 K. oxytoca P2 E. coli KO11 E. coli KO11 E. coli KO11 E. coli KO11 E. coli KO11 K. oxytoca P2 E. coli KO11 E. coli LY01 E. coli KO11

Dilute acid hydrolysis Dilute acid hydrolysis Enzymes only Dilute acid hydrolysis Grinding, enzymes, filtration Dilute acid pulped Dilute acid hydrolysis Dilute acid hydrolysis Dilute acid hydrolysis Dilute acid hydrolysis Enzymes only Enzymes only Proteinase Pressurized batch hot water Dilute acid hydrolysis

0.51 0.48 0.47 0.39b 0.41 0.43 0.46–0.48 0.45–0.53 0.44–0.51 0.46 0.32–0.41b 0.20b 0.43 0.40 0.45

Beall et al. (1992) Barbosa et al. (1992) Doran and Ingram (1993) Doran et al. (1994) Grohmann et al. (1994) Brooks (1995) Asghari et al. (1996) Asghari et al. (1996) Asghari et al. (1996) Moniruzzaman and Ingram (1998) Doran et al. (2000) Sutton and Doran Peterson (2001) Leite et al. (2000) Brandon et al. (2007) Okuda et al. (2007)

g TE g)1 S , grams of total ethanol per gram of sugar. Grams of total ethanol divided by grams of treated biomass on a dry weight basis.



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588 Joy Doran-Peterson et al.


Ethanol (g l)1)

Yield (g TE g)1 S)a

E. coli KO11



E. coli LY01 E. coli FBR5 (pLOI297) K. oxytoca M5A1 (pLOI555) Z. mobilis CP4 (pZB5) Z. mobilis CP4 (pZB5) Saccharomyces sp 1400 (pLNH32) S. cerevisiae RE700A (pKDR) S. cerevisiae RWB202-AFX S. cerevisiae RWB217 S. cerevisiaeTMB3400 Pichia stipitis CBS 6054

42.4 41.5 46.0 36.6 23.0 23.0 23.0 8.6 8.7 3.5 3.7

0.47 0.44 0.46 0.42 0.38 0.46 0.46 0.43 0.44 0.25 0.31


Table 4 Comparison of ethanol yield from xylose

L.O. Ingram (University of Florida, USA) personal communication Yomano et al. (1998) Dien et al. (2000) Ohta et al. (1991) Lawford and Rousseau (1999) Lawford and Rousseau (1999) Ho et al. (2000) Sedlak and Ho (2004) Kuyper et al. (2004) Kuyper et al. (2005) Wahlbom et al. (2003) Wahlbom et al. (2003)

g TE g)1 S, grams of total ethanol per gram of sugar.


pre-treated grass) was obtained after pre-treatment at 230C for 2 min with a very low fungal cellulase load. Other ethanologenic E. coli strains E. coli K12 strains were engineered with the same Z. mobilis alcohol pathway genes (pet operon) used in engineering strain KO11, and are designated FBR (Fermentation Biochemistry Research Unit of the US Department of Agriculture). In these strains, pyruvate reduction is blocked and the NADH,H+ recycling from glycolysis is limited due to pfl (pyruvate formate lyase) and ldh (lactate dehydrogenase) mutations (Figure 3). Adding the pet operon on a plasmid restored fermentative growth, such that the cell is obliged to retain the plasmid to support growth (Dien et al., 2000; Hespell et al., 1996). E. coli strain FBR5 produced ethanol from various substrates at 86–92% of the theoretical yield (Dien et al., 2000). All E. coli strains discussed previously expressed the Z. mobilis ethanol pathway; however, ethanol production by an E. coli strain lacking foreign genes has been recently described (Kim et al., 2007). A mutant generated by exposure to chemicals from an anaerobic growth-defective strain (DldhA/DpflB) was isolated that is capable of anaerobic growth. This strain, SE2378, has a mutation within the pyruvate dehydrogenase (pdh) operon, and fermented xylose and glucose to ethanol with 82% yield. This pdh operon mutation produced an additional NADH for each pyruvate, allowing the balanced production of two moles of ethanol per mole of glucose. Zhou et al. (2008) recently described engineering of a native homoethanol pathway (pyruvate to acetyl CoA to acetaldehyde to ethanol) in E. coli B. Chromosomal deletions of the genes encoding lactate dehydrogenase (ldhA), fumarate reductase (frdABCD), acetate kinase (ackA) and pyruvate formate

lyase (pflB) removed competing fermentation pathways. The pyruvate dehydrogenase complex (aceEF–lpd) was highly expressed anaerobically using a native anaerobic inducible promoter and resulted in a strain containing no foreign genes. This new strain, SZ420, efficiently fermented glucose and xylose into ethanol with a yield of 90% under anaerobic conditions.

Conclusion In the fermentation platform, plant biomass substrate cost is reduced by using all of the plant-derived sugars available, C5 and C6, for conversion to ethanol. There is no single naturally occurring microbial biocatalyst that effectively and efficiently converts all C5 and C6 plant-derived sugars to one product with high yields, be it lactic acid or ethanol. This has lead to a number of laboratories working to engineer robust biocatalysts for industrial production of lactic acid or ethanol using both yeast and bacterial metabolic processes and enzymes. Significant progress has been made in enhancing conversion of plant biomass sugars to lactic acid and ethanol, and further improvements in enzyme efficiency, biocatalyst tolerance to biomass inhibitors, and process step consolidation will increase the economic viability of harnessing plant biomass for biofuels and commodity chemicals. References Agrawal, A.K. and Bhalla, R. (2003) Advances in the production of poly(lactic acid) fibres. A review. J. Macromol. Sci. 43, 479– 504. Almeida, J.P.M., Modig, T., Petersson, A., Hahn-Hagerdahl, B., Linden, G. and Gorwa-Grauslund, M.F. (2007) Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae. J. Chem. Technol. Biotechnol. 82, 340–349.

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Microbial conversion of sugars from plant biomass 589 Antoni, D., Zverlov, V. and Schwarz, W. (2007) Biofuels from microbes. Appl. Microbiol. Biotechnol. 77, 23–35. Asghari, A., Bothast, R.J., Doran, J.B. and Ingram, L.O. (1996) Ethanol production of hemicellulose hydrolysates of agricultural residue using genetically engineered Escherichia coli strain KO11. J. Indust. Microbiol. 21–26, 21–26. Barbosa, M.F., Beck, M.J., Fein, J.E., Potts, D. and Ingram, L.O. (1992) Efficient fermentation of Pinus sp. acid hydrolysates by an ethanologenic strain of Escherichia coli. Appl. Environ. Microbiol. 58, 1382–1384. Beall, D.S., Ohta, K. and Ingram, L.O. (1991) Parametric studies of ethanol production from xylose and other sugars by recombinant Escherichia coli. Biotechnol. Bioeng. 38, 269–303. Beall, D.S., Ingram, L.O., Ben-Bassat, A., Doran, J.B., Fowler, D.E., Hall, R.G. and Wood, B.E. (1992) Conversion of hydrolysates of corn cobs and hulls into ethanol by recombinant Escherichia coli B containing integrated genes for ethanol production. Biotechnol. Lett. 14, 857–862. Bianchi, M.M., Brambilla, L., Protani, F., Liu, C.L., Lievense, J.C. and Porro, D. (2001) Efficient homolactic fermentation by Kluveromyces lactis strains defective in pyruvate utilization and transformed with heterologous LDH gene. Appl. Environ. Microbiol. 67, 5621–5625. Bolotin, A., Wincker, P., Mauger, S., Jaillon, O., Malarme, K., Weissenback, J., Ehrlich, S.D. and Sorokin, A. (2001) The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp IL1403. Genome Res. 11, 731–753. Boopathy, R., Bokang, H. and Daniels, L. (1993) Biotransformation of furfural and 5-hydroxymethylfurfural by enteric bacteria. J. Indust. Microbiol. 11, 147–150. Bothast, R.J. and Schlicher, M.A. (2005) Biotechnological processes for conversion of corn into ethanol. Appl. Microbiol. Biotechnol. 67, 19–25. Brandon, S.K., Eiteman, M.A., Patel, K., Richbourg, M.M., Miller, D.J., Anderson, W.F. and Peterson, J.D. (2007) Hydrolysis of Tifton 85 bermudagrass in a pressurized batch hot water reactor. J. Chem. Technol. Biotechnol. 83, 505–512. Brooks, T.A. (1995) Conversion of mixed waste office paper to ethanol by genetically engineered Klebsiella oxytoca strain P2. Biotechnol. Prog. 11, 619–625. Chang, D.E., Jung, H.C., Rhee, J.S. and Pan, J.G. (1999) Homofermentative production of D- or L-lactate in metabolically engineered Escherichia coli RR1. Appl. Environ. Microbiol. 65, 1384–1389. Clark, D.P. (1989) The fermentation pathways of Escherichia coli. FEMS Microbiol. Rev. 5, 223–234. Cocaign-Bousquet, M., Garrigues, C., Loubiere, P. and Lindley, N.D. (1996) Physiology of pyruvate metabolism in Lactococcus lactis. Antonie van Leeuwenhoek 70, 253–267. Cock, L.S. and de Stouvenel, A.R. (2006) Lactic acid production by a strain of Lactococcus lactis subs lactis isolated from sugar cane plants. Electron. J. Biotechnol. 9, 40–45. Datta, R. and Henry, M. (2006) Lactic acid: recent advances in products, processes and technologies – a review. J. Chem. Technol. Biotechnol. 81, 1119–1129. Delgenes, J.P., Moletta, R. and Navarro, J.M. (1996) Effects of lignocellulose degradation products on ethanol fermentations of glucose and xylose by Saccharomyces cerevisiae, Zymomonas mobilis, Pichia stipitis, and Candida shehatae. Enzyme Microb. Technol. 19, 220–225. Demirci, A. and Pometto, A.L. (1992) Enhanced production of D())-lactic acid by mutants of Lactobacillus delbrueckii ATCC9649. J. Indust. Microbiol. 11, 23–28. Dien, B.S., Nichols, N.N., O’Bryan, P.J. and Bothast, R.J. (2000) Development of new ethanologenic Escherichia coli strains for

fermentation of lignocellulosic biomass. Appl. Biochem. Biotechnol. 84, 181–196. Dien, B.S., Nichols, N.N. and Bothast, R.J. (2001) Recombinant Escherichia coli engineered for production of L-lactic acid from hexose and pentose sugars. J. Indust. Microbiol. Biotechnol. 27, 259–264. Dien, B.S., Cotta, M.A. and Jeffries, T.W. (2003) Bacteria engineered for fuel ethanol production: current status. Appl. Microbiol. Biotechnol. 63, 258–266. Doran, J.B. and Ingram, L.O. (1993) Fermentation of crystalline cellulose to ethanol by Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes. Biotechnol. Prog. 9, 533–538. Doran, J.B., Aldrich, H.C. and Ingram, L.O. (1994) Saccharification and fermentation of sugar cane bagasse by Klebsiella oxytoca P2 containing chromosomally integrated genes encoding the Zymomonas mobilis ethanol pathway. Biotechnol. Bioeng. 44, 240–247. Doran, J.B., Cripe, J., Sutton, M. and Foster, B. (2000) Fermentations of pectin-rich biomass with recombinant bacteria to produce fuel ethanol. Appl. Biochem. Biotechnol. 84–86, 141–152. Drake, H.L. and Daniel, S.L. (2004) Physiology of the thermophilic acetogen Moorella thermoacetica. Res. Microbiol. 155, 869– 883. Dupreez, J.C. (1994) Process parameters and environmental-factors affecting D-xylose fermentation by yeasts. Enzyme Microb. Technol. 16, 944–956. Eddington, J.M., Johnson, K.B., Liaw, H.J., Rowe, M. and Yang, Y. (2004) Lactobacillus Strains and the Use Thereof in Fermentation Process for Lactic Acid Production. USA Patent 7, 300, 787. Feldmann, S.D., Sahm, H. and Sprenger, G.A. (1992) Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains. Appl. Microbiol. Biotechnol. 38, 354–361. Grabar, T.B., Zhou, S., Shanmugam, K.T., Yomano, L.P. and Ingram, L.O. (2006) Methylglyoxal bypass identified as a source of chiral contamination in L(+) and D())-lactate fermentations by recombinant Escherichia coli. Biotechnol. Lett. 28, 1527–1535. Grohmann, K., Baldwin, E.A., Buslig, B.S. and Ingram, L.O. (1994) Fermentation of galacturonic acid and other sugars in orange peel hydrolysates by the ethanologenic strain of Escherichia coli. Biotechnol. Lett. 16, 281–286. Gutierrez, T., Buszko, M.L., Ingram, L.O. and Preston, J.F. (2002) Reduction of furfural to furfuryl alcohol by ethanologenic strains of bacteria and its effect on ethanol production from xylose. Appl. Biochem. Biotechnol. 98–100, 327–340. Hahn-Ha¨gerdal, B., Hallborn, J., Jeppson, H., Olsson, L., Skoog, K. and Walfridsson, M. (1993) Pentose fermentation to alcohol. Biotechnol. Agric. 9, 231–290. Hahn-Ha¨gerdal, B., Galbe, M., Gorwa-Grauslund, M.F., Linde´n, G. and Zacchi, G. (2006) Bio-ethanol – the fuel of tomorrow from the residues of today. Trends Biotechnol. 24, 549–556. Hahn-Ha¨gerdal, B., Karhumaa, K., Fonseca, C., Spencer-Martins, I. and Gorwa-Grauslund, M. (2007) Towards industrial pentosefermenting yeast strains. Appl. Biochem. Biotechnol. 74, 937– 953. Hamelinck, C.N., von Hooijdonk, G. and Faaij, A.P.C. (2005) Ethanol from lignocellulosic biomass: techno-economic performance in short-, middle- and long-term. Biomass Bioenergy, 28, 384–410. Hartley, R. and Ford, C. (1989) Phenolic constituents of plant cell walls and wall degradability. In Plant Cell Wall Polymers: Biogenesis and Biodegradation. American Chemical Society Symposium Series (Lewis, N. and Paice, M., eds). Washington, DC: American Chemical Society, pp. 137–144.

ª 2008 The Authors Journal compilation ª 2008 Blackwell Publishing Ltd, The Plant Journal, (2008), 54, 582–592

590 Joy Doran-Peterson et al. Hespell, R.B., Wyckoff, H., Dien, B.S. and Bothast, R.J. (1996) Stabilization of pet operon plasmids and ethanol production in Escherichia coli strains lacking lactate dehydrogenase and pyruvate formate-lyase activities. Appl. Environ. Microbiol. 62, 4594–4597. Ho, N.W.Y., Chen, Z., Brainard, A.P. and Sedlak, M. (2000) Genetically engineered Saccharomyces yeasts for conversion of cellulosic biomass to environmentally friendly transportation fuel ethanol. In Green Chemical Syntheses and Processes (Anastas, P.T., Heine, L.G. and Williamson, T.C., eds). Washington, DC: American Chemical Society, pp. 143–159. Hofvendahl, K. and Hans-Hagerdal, B. (2000) Factors affecting the fermentative lactic acid production from renewable resources. Enzyme Microb. Technol. 26, 87–107. Huang, Y.L., Mann, K., Novak, J.M. and Yang, S.T. (1998) Acetic acid production from fructose by Clostridium formicoaceticum immobilized in a fibrous-bed bioreactor. Biotechnol. Prog. 14, 800–806. Ilmen, M., Koivuranta, K., Ruohonen, L., Suominen, P. and Penttila, M. (2007) Efficient production of L-lactic acid from xylose by Pichia stipitis. Appl. Environ. Microbiol. 73, 117–123. Ingram, L.O., Lai, X. and Moniruzzaman, M. (1997) Fuel ethanol production from lignocellulose using genetically engineered bacteria. In Fuels and Chemicals from Biomass (Saha, B. and Woodward, J., eds). Washington, DC: American Chemical Society, pp. 57–63. Ingram, L.O., Aldrich, H.C., Borges, A.C. et al. (1999) Enteric bacterial catalysts for fuel ethanol production. Biotechnol. Prog. 15, 855–866. Iyer, P.V., Thomas, S. and Lee, Y.Y. (2000) High-yield fermentation of pentoses into lactic acid. Appl. Biochem. Biotechnol. 84–86, 665–677. Jeffries, T.W. (2006) Engineering yeasts for xylose metabolism. Curr. Opin. Biotechnol. 17, 320–326. Jeffries, T.W. and Jin, Y.S. (2004) Metabolic engineering for improved fermentation of pentoses by yeasts. Appl. Microbiol. Biotechnol. 63, 495–509. Jeoh, T. and Agblevor, F.A. (2001) Characterization and fermentation of steam exploded cotton gin waste. Biomass Bioenergy, 21, 109–120. Joachimsthal, E.L. and Rogers, P.L. (2000) Characterization of a high-productivity recombinant strain of Zymomonas mobilis for ethanol production from glucose/xylose mixtures. Appl. Biochem. Biotechnol. 77, 147–157. Jones, D.T. and Woods, D.R. (1986) Acetone–butanol fermentation revisited. Microbiol. Rev. 50, 484–525. Kenealy, W.R., Houtman, C.J., Laplaza, J., Jeffries, T.W. and Horn, E.G. (2007) Pretreatments for converting wood into paper and chemicals. In Materials, Chemicals, and Energy From Forest Biomass (Argyropoulos, D.S., ed.). Washington, DC: American Chemical Society, pp. 392–408. Kim, Y., Ingram, L.O. and Shanmugam, K.T. (2007) Construction of an Escherichia coli K-12 mutant for homoethanologenic fermentation of glucose or xylose without foreign genes. Appl. Environ. Microbiol. 73, 1766–1771. Kotter, P. and Ciriacy, M. (1993) Xylose fermentation by Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 38, 776–783. Kuyper, M., Harhangi, H.R., Stave, A.K., Winkler, A.A., Jetten, M.S.M., de Laat, W.T.A.M., den Ridder, J.J.J., Op den Camp, H.J.M., van Dijken, J.P. and Pronk, J.T. (2003) High-level functional expression of a fungal xylose isomerase: the key to efficient ethanolic fermentation of xylose by Saccharomyces cerevisiae. FEMS Yeast Res. 4, 69–78.

Kuyper, M., Winkler, A.A., van Dijken, J.P. and Pronk, J.T. (2004) Minimal metabolic engineering of Saccharomyces cerevisiae for efficient anaerobic xylose fermentation: a proof of principle. FEMS Yeast Res. 4, 655–664. Kuyper, M., Toirkens, M.J., Diderich, J.A., Winkler, A.A., van Dijken, J.P. and Pronk, J.T. (2005) Evolutionary engineering of mixedsugar utilization by a xylose-fermenting Saccharomyces cerevisiae strain. FEMS Yeast Res. 5, 925–934. Kyla-Nikkila, K., Hujanen, M., Leisola, M. and Palva, A. (2000) Metabolic engineering of Lactobacillus helveticus CNR32 for production of pure L(+)-lactic acid. Appl. Environ. Microbiol. 66, 3835–3841. Larsson, S., Quintana-Sainz, A., Reimann, A., Nilvebrant, N.-O. and Jonsson, L.J. (2000) Influence of lignocellulose-derived aromatic compounds on oxygen-limited growth and ethanolic fermentation by Saccharomyces cerevisiae. Appl. Biochem. Biotechnol. 84–86, 617–632. Lau, M.W., Dale, B.E. and Balan, V. (2008) Ethanolic fermentation of hydrolysates from ammonia fiber expansion (AFEX) treated corn stover and distillers grain without detoxification and external nutrient supplementation. Biotechnol. Bioeng. 99, 529– 539. Lawford, H.G. and Rousseau, J.D. (1999) The effect of glucose on high-level xylose fermentations by recombinant Zymomonas in batch and fed-batch fermentations. Appl. Biochem. Biotechnol. 77–79, 235–249. Lee, S.Y., Hong, S.H., Lee, S.H. and Park, S.J. (2004) Fermentative production of chemicals that can be used for polymer synthesis. Macromol. Biosci. 4, 157–164. Leite, A.R., Guimaraes, W.V., de Araujo, E.F. and Silva, D.O. (2000) Fermentation of sweet whey by recombinant Escherichia coli KO11. Braz. J. Microbiol. 31, 212–215. Lequart, C., Ruel, K., Lapierre, C., Pollet, B. and Kurek, B. (2000) Abiotic and enzymatic degradation of wheat straw cell wall: a biochemical and ultrastructural investigation. J. Biotechnol. 80, 249–259. Li, X., Weng, J.-K. and Chapple, C. (2008) Tailoring lignin for the improvement of forage, pulp, and biofuel: from genetics to genetic engineering. Plant J. 54, 569–581. Lin, Y. and Tanaka, S. (2006) Ethanol fermentation from biomass resources: current state and prospects. Appl. Microbiol. Biotechnol. 69, 627–642. Liu, C.L. and Lievense, J.C. (2005) Lactic Acid-Producing Yeast. USA Patent 20050112737. Liu, S., Skinner-Nemec, K.A. and Leathers, T.D. (2008) Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of xylose and glucose into ethanol and other products. J. Indust. Microbiol. Biotechnol. 35, 75–81. Lynd, L.R., van Zyl, W.H., McBride, J.E. and Laser, M. (2005) Consolidated bioprocessing of cellulosic biomass: an update. Curr. Opin. Biotechnol. 16, 577–583. Makarova, K., Slesarev, A., Wolf, Y. et al. (2006) Comparative genomics of the lactic acid bacteria. Proc. Natl Acad. Sci. USA, 102, 15611–15616. van Maris, A.J., Winkler, A.A., Porro, D., van Dijken, J.P. and Pronk, J.T. (2004) Homofermentative lactate production cannot sustain anaerobic growth of engineered Saccharomyces cerevisiae: possible consequence of energy-dependent lactate export. Appl. Environ. Microbiol. 70, 2898–2905. van Maris, A., Winkler, A., Kuyper, M., de Laat, W., van Dijken, J. and Pronk., J.T. (2007) Development of efficient xylose fermentation in Saccharomyces cerevisiae: xylose isomerases as a key component. Adv. Biochem. Eng. Biotechnol. 108, 179–204.

ª 2008 The Authors Journal compilation ª 2008 Blackwell Publishing Ltd, The Plant Journal, (2008), 54, 582–592

Microbial conversion of sugars from plant biomass 591 McKinlay, J.B., Vieille, C. and Zeikus, G. (2007) Prospects for biobased succinate industry. Appl. Microbiol. Biotechnol. 76, 727– 740. McMillan, J.D. (1994a) Conversion of hemicellulose hydrolysates to ethanol. In Enzymatic Conversion of Biomass for Fuels Production (Himmel, M.E., Bake, J.O. and Overend, R.P., eds). Washington, DC: American Chemical Society, pp. 411–437. McMillan, J.D. (1994b) Pretreatment of lignocellulosic biomass. In Enzymatic Conversion of Biomass for Fuels Production (Himmel, M.E., Bake, J.O. and Overend, R.P., eds). Washington, DC: American Chemical Society, pp. 292–324. Mohagheghi, A., Evans, K., Chou, Y.-C. and Zhang, M. (2002) Co-fermentation of glucose, xylose, and arabinose by genomic DNA-integrated xylose/arabinose fermenting strain of Zymomonas mobilis AX101. Appl. Biochem. Biotechnol. 98–100, 885–898. Moniruzzaman, M. and Ingram, L.O. (1998) Ethanol production from dilute acid hydrolysate of rice hulls using genetically engineered Escherichia coli. Biotechnol. Lett. 20, 943–947. Moniruzzaman, M., Dien, B.S., Ferrer, B., Hespell, R.B., Dale, B., Ingram, L.O. and Bothast, R.J. (1996) Ethanol production from AFEX pretreated corn fiber by recombinant bacteria. Biotechnol. Lett. 18, 985–990. Mosier, N., Wyman, C., Dale, B., Elander, R., Lee, Y.Y., Holtzapple, M. and Ladisch, M. (2005) Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour. Technol. 96, 673–686. Mussatto, S.I. and Roberto, I.C. (2004) Alternatives for detoxification of diluted-acid lignocellulosic hydrolyzates for use in fermentative processes: a review. Bioresour. Technol. 93, 1–10. Narayanan, N., Roychoudhury, P.K. and Srivastava, A. (2004) L-(+)lactic acid fermentation and its product polymerization. Electron. J. Biotechnol. 7, 167–179. Ohta, K., Beall, D.S., Mejia, J.P., Shanmugam, K.T. and Ingram, L.O. (1991) Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II. Appl. Environ. Microbiol. 57, 893–900. Okuda, N., Ninomiya, K., Takao, M., Katakura, Y. and Shioya, S. (2007) Microaeration enhances productivity of bioethanol from hydrolysate of waste house wood using ethanologenic Escherichia coli KO11. J. Biosci. Bioeng. 4, 350–357. Ostergaard, S., Olsson, L. and Nielsen, J. (2000) Metabolic engineering of Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 64, 34–50. Pauly, M. and Keegstra, K. (2008) Cell-wall carbohydrates and their modifications as a resource for biofuels. Plant J. 54, 559–568. Pieterse, B., Leer, R.J., Schuren, F.H.J. and van der Werf, M.J. (2005) Unravelling the multiple effects of lactic acid stress on Lactobacillus plantarum by transcription profiling. Microbiology, 151, 3881–3894. Porro, D., Michele, M.B., Luca, B. et al. (1999) Replacement of a metabolic pathway for large-scale production of lactic acid from engineered yeasts. Appl. Environ. Microbiol. 65, 4211– 4215. Ragauskas, A.J., Williams, C.K., Davison, B.H. et al. (2006) The path forward for biofuels and biomaterials. Science, 311, 484– 489. Rajgarhia, V., Dundon, C.A., Olson, S., Suominen, P. and Hause, B. (2004) Methods and Materials for the Production of D-Lactic Acid in Yeast. USA Patent 20040029256. Rao, K., Chaudhari, V., Varanasi, S. and Kim, D.-S. (2007) Enhanced ethanol fermentation of brewery wastewater using the genetically modified strain E. coli KO11. Appl. Microbiol. Biotechnol. 74, 50–60.

Ray, S.S. and Bousmina, M. (2005) Biodegradable polymers and their layered silicate nanocomposites: greening the 21st century materials world. Prog. Mater. Sci. 50, 962–1079. Richard, P., Verho, R., Putkonen, M., Londesborough, J. and Penttila, M. (2003) Production of ethanol from L-arabinose by Saccharomyces cerevisiae containing a fungal L-arabinose pathway. FEMS Yeast Res. 3, 185–189. Rose, J.K.C., O’Neil, M.A., Albersheim, P. and Darvill, A. (2000) The primary cell wall of higher plants. In Carbohydrates in Chemistry and Biology (Ernst, B., Hart, G.W. and Sinay, P., eds). Weinheim, Germany: Wiley-VCH, pp. 783–808. Saitoh, S., Ishida, N., Onishi, T., Tokuhiro, N., Nagamori, E., Kitamota, K. and Takahashi, H. (2005) Genetically engineered wine yeast produces a high concentration of L-lactic acid of extremely high optical purity. Appl. Environ. Microbiol. 71, 2789–2792. Sedlak, M. and Ho, N.W.Y. (2001) Expression of E.coli araBAD operon encoding enzymes for metabolizing L-arabinose in Saccharomyces cerevisiae. Enzyme Microb. Technol. 28, 16–24. Sedlak, M. and Ho, N.W.Y. (2004) Characterization of the effectiveness of hexose transporters for transporting xylose during glucose and xylose co-fermentation by a recombinant Saccharomyces yeast. Yeast, 21, 671–684. Sheehan, J., Aden, A., Paustian, K., Killian, K., Brenner, J., Walsh, M. and Nelson, R. (2004) Energy and environmental aspects of using corn stover for fuel ethanol. J. Indust. Ecol. 7, 117–146. Singh, S.K., Ahmed, S.U. and Pandey, A. (2006) Metabolic engineering approaches for lactic acid production. Process Biochem. 41, 991–1000. Skory, C. (2000) Isolation and expression of lactate dehydrogenase genes from Rhizopus oryzae. Appl. Environ. Microbiol. 66, 2343– 2348. Smith, A. (2008) Prospects for increasing starch and sucrose yields for bioethanol production. Plant J. 54, 546–558. Sommerville, D., Bradley, R. and Mailly, D. (2004) Leaf litter quality and decomposition rates of yellow birch and sugar maple seedlings grown in mono-culture and mixed-culture pots at three soil fertility levels. Trees, 18, 608–613. Sun, Y. and Cheng, J. (2005) Dilute acid pretreatment of rye straw and bermudagrass for ethanol production. Bioresour. Technol. 96, 1599–1606. Sutton, M. and Doran Peterson, J. (2001) Fermentation of sugarbeet pulp for ethanol production using bioengineered Klebsiella oxytoca strain P2. J. Sugar Beet Res. 38, 19–34. Tanaka, K., Komiyama, A., Sonotomoto, K., Ishizaki, A., Hall, S.J. and Stanbury, P.F. (2002) Two different pathways for D-xylose metabolism and the effect of xylose concentration on the yield coefficient of L-lactate in mixed-acid fermentation by the lactic acid bacterium Lactococcus lactis IO-1. Appl. Microbiol. Biotechnol. 60, 160–167. Vincken, J.-P., Schols, H.A., Oomen, R.F.J., McCann, M.C., Ulvskov, P., Voragen, A.G.J. and Visser, R.G.F. (2003) If homogalacturonan were a side chain of rhamnogalacturonan I. Implications for cell wall architecture. Plant Physiol. 132, 1781–1789. Wahlbom, C.F., van Zyl, W.H., Jo¨nsson, L.J., Hahn-Ha¨gerdal, B. and Otero, R.R. (2003) Generation of the improved recombinant xylose-utilizing Saccharomyces cerevisiae TMB 3400 by random mutagenesis and physiological comparison with Pichia stipitis CBS 6054. FEMS Yeast Res. 3, 319–326. Walfridsson, M., Bao, M., Anderlund, X., Lilius, G., Bu¨low, L. and Hahn-Ha¨gerdal, B. (1996) Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase. Appl. Environ. Microbiol. 62, 4648–4651.

ª 2008 The Authors Journal compilation ª 2008 Blackwell Publishing Ltd, The Plant Journal, (2008), 54, 582–592

592 Joy Doran-Peterson et al. Wyman, C. (ed.) (1996) Handbook on Bioethanol: Production and Utilization. Applied Energy Technology Series. Washington, DC: Taylor & Francis. Wyman, C.E. (2003) Potential synergies and challenges in refining cellulosic biomass to fuels, chemicals, and power. Biotechnol. Prog. 19, 254–262. Yomano, L.P., York, S.W. and Ingram, L.O. (1998) Isolation and characterization of ethanol-tolerant mutants of Escherichia coli KO11 for fuel ethanol production. J. Indust. Microbiol. Biotechnol. 20, 132–138. Zaldivar, J. and Ingram, L.O. (1999) Effect of organic acids on the growth and fermentation of ethanologenic Escherichia coli LY01. Biotechnol. Bioeng. 66, 203–210. Zaldivar, J., Martinez, A. and Ingram, L.O. (1999) Effect of selected aldehydes on the growth and fermentation of ethanologenic Escherichia coli. Biotechnol. Bioeng. 65, 24–33. Zaldivar, J., Nielsen, J. and Olsson, L. (2001) Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration. Appl. Microbiol. Biotechnol. 56, 17–34.

Zhang, M., Eddy, C., Deanda, K., Finkelstein, M. and Picataggio, S. (1995) Metabolic engineering of a pentose metabolism pathway in ethanologenic Zymomonas mobilis. Science, 267, 240– 243. Zhou, S., Causey, T.B., Hasona, A., Shanmugam, K.T. and Ingram, L.O. (2003a) Production of optically pure D-lactic acid in mineral salts medium by metabolically engineered Escherichia coli W3110. Appl. Environ. Microbiol. 69, 399–407. Zhou, S., Shanmugam, K.T. and Ingram, L.O. (2003b) Functional replacement of the Escherichia coli D-())-lactate dehydrogenase gene (ldhA) with the L-(+)-lactate dehydrogenase gene (ldhL) from Pediococcus acidilactici. Appl. Environ. Microbiol. 69, 2237– 2244. Zhou, S.G., Causey, T.B., Yomano, L.P., Shanmugam, K.T. and Ingram, L.O. (2005) Fermentation of 10% (w/v) sugar to D())-lactate by engineered Escherichia coli B. Biotechnol. Lett. 27, 1891– 1896. Zhou, S., Iverson, A.G. and Grayburn, W.S. (2008) Engineering a native homoethanol pathway in Escherichia coli B for ethanol production. Biotechnol. Lett. 30, 335–342.

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Microbial conversion of sugars from plant ... - Wiley Online Library

selected chemicals and fuels that can be produced from microbial fermentation of plant-derived cell-wall sugars and directed engineering for improvement of microbial biocatalysts. Lactic acid and ethanol production are highlighted, with a focus on engineered Escherichia coli. Keywords: plant sugars, ethanol, lactic acid, ...

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