St. Joseph’s College of Arts & Science (Autonomous) St. Joseph’s College Road, Cuddalore – 607001 BC303 - INTERMEDIARY METABOLISM - I
Time : 3 hrs
Max Marks :75 SECTION – A (25X1=25) Answer ALL Questions
I. Choose the correct answer 1. The protein part of a holoenzyme is termed as a) Apoenzyme b) Coenzyme c) Cofactor
d) All of the above
2. Which class of enzyme do not exhibit stereospecificity? a) Isomerases b) Lyases c) Hydrolases d) Transferases 3. Which explains the kinetics of an enzyme a) Michaelis - Menten equation b) Lineweaver - Burk plot c) Hill plot d) All of these 4. Maximum enzyme activity is observed at a) Acidic pH b) Neutral pH c) Basic pH
d) Optimum Ph
5. PDH contains all except a) Biotin b) NAD
d) CoA
c) FAD
6. In glycogenesis, the branching enzyme is called as a) Amylo 1-6 transglucosidase b) Amylo (1-4 )- (1-6) transglucosidase c) Alpha 1-6 glucosidase d) UDP- glucose pyrophosphorylase 7. Glyoxalate cycle take place in a) Man and animals b) Animals and plants c) Plants and microbes d) Microbes 8. The connecting link between HMP shunt and lipid synthesis is a) Ribose b) NADPH c) NADH d) Sedoheptulose-7 phosphate
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9. The structure of CoQ is similar to a) Vitamin K b) Vitamin D c) Vitamin A
d) Vitamin B1
10. The number of coupling sites in ETC are a) One b) Two c) Three d) Four II. True or False 11. Coenzymes are regarded as second substrate. 12. Competitive inhibitor binds to allosteric site of enzymes. 13. TCA cycle operates in mitochondrial matrix. 14. HMP shunt produces ATP molecules. 15. Uncouplers inhibit electron transfer in ETC. III. Answer in One or Two Sentences 16. What is Allosteric site? 17. Define P:O ratio. 18. What is Q10? 19. What is Debranching enzyme? 20. Mention the enzymes present in PDH complex. 21. Glycogen synthase. 22. Write the reaction catalysed by isocitrate lyase. 23. What is the importance of Line weaver Burk equation? 24. Write any two transporter systems in inner mitochondrial membrane. 25. Write the function of Coenzyme Q.
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SECTION – B (4X5=20) Answer any FOUR Questions 26. Write briefly on induced fit model of enzyme action. 27. What are isoenzymes? Give examples. 28. Write briefly on specificity of enzymes. 29. Write the reactions of TCA cycle. 30. What are uncouplers? Give examples. 31. Explain glycerophosphate shuttle system. SECTION – C (3X10=30) Answer any THREE Questions 32. Explain the factors that influence enzyme activity. 33. Elaborate on enzyme inhibition and its types. 34. Describe the steps of glycogenesis. 35. Discuss in detail the reactions of gluconeogenesis. 36. Write in detail the chemiosmotic theory.
a) Michaelis - Menten equation b) Lineweaver - Burk plot. c) Hill plot d) All of these. 4. Maximum enzyme activity is observed at. a) Acidic pH b) Neutral pH c) ...
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1. Choose the correct answer: (10X1=10). 1. D-galactose and D-glucose are. (a) epimers of each other. (b) enantiomers of each other. (c) anomers of each other.
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Page 1 of 2. By Emma Scott,. Emelia Skogstad. and Grace Isaacson. An ad-room was held to. begin the day today as students. and staff prepare for standard- ized testing of juniors to begin. Wednesday. On Wednesday, juniors. will take the S.D. Science