ANGELA MARIA RIZZO

INSTITUTION(S) AND LOCATION

BIOGRAPHICAL SKETCH POSITION TITLE ASSISTANT PROFESSOR OF BIOCHEMISTRY EDUCATION/TRAINING DEGREE(S) (if applicable)

YEAR(S)

FIELD(S) STUDY

University of Milan

Degree in Pharmaceutical Chemistry

1989

Molecular Biology

University of Milan

Degree Pharmacy

in

1992

Biochemistry

University of Milan

PhD Biochemistry

in

1997

Biochemistry

OF

YEAR(S)

PROFESSIONAL EXPERIENCE AND HONORS

1989

Research fellow from University of Milan.

1990

Research Fellow to the Department of Pharmacology, Medical School University of Minnesota in Minneapolis MN USA

1997

Post Doc Fellow University of Milan.

1998

Researcher in Biochemistry University of Milan Faculty of Pharmacy

From 1999

Assistant professor in Biochemistry, Faculty of Pharmacy University of Milan..

2007

Member of the Working Group of Medicine and Biotechnology of the Italian Space Agency for PTA e PASN development

2008

Reviewer for the Science Foundation (ESF) and its Space Sciences Expert Committee (ESSC) to carry out an independent scientific evaluation of the ELIPS programme Member of the ELGRA (European Low Gravity Research Association) Member of the SIB (Italian Society of Biochemistry) Reviewer for many international journals

Research Areas: a) Glycolipids and cell membrane metabolisms. b) Biochemical and nutraceutical systems against oxidative stress c) Omega-3 fatty acid involvement in psychological issues d) Omega-3 fatty acid involvement in cancer progression and degenerative diseases. e) Space biology f) Nutrition SPACE RESEARCH RELATED PROJECTS PARTICIPATION OR COORDINATION Project funded by ASI: - X.E.CO.RA: Effects of spatial radiation on the development of XENOPUS laevis P.I. Angela M. Rizzo

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Topical Team in human nutrition in microgravity Coordinator Prof. Di Nardo. In vitro and in vivo effects of microgravity on cell membrane P.I. Prof. Bruno Berra Effects of cosmic radiation on skin cells. (RA.SKIN) PI Prof. Bruno Berra MoMa “From Molecules to Man: Space Research Applied to the improvement of the Quality of Life of the Ageing Population on Earth National coordination Prof. Saverio Ambesi Impiombato Dr. A.M. Rizzo had the Italian coordination of ASSC line: “ANIMAL MODELS TO STUDY DELETERIOUS EFFECTS OF SPACE AND POSSIBLE CONTERMEASURES” (ASSC) Omega-3 polyunsaturated fatty acid and psychological wellness during long duration space missions. AM RIZZO PI

Projects accepted by NASA/ESA: - EFFECTS OF SPACE FLIGHT ON ERYTHROCYTES, ANDROGEN LEVELS AND OXIDATIVE STRESS OF RATS FLOWN ON STS-107 - MARS 500: Omega-3 polyunsaturated fatty acid and psychological wellness during long duration space missions She was also involved as Unit responsible in VI and VII PQ EU nutrition related projects SPACE FLIGHT Experiences: 2000- 2003 Stratospheric Balloon flights BI.R.BA, BI.R.BA I, BI.R.BA II. 2003- Shuttle flight, STS-107: “Effects of space flight on erythrocytes, androgen levels and oxidative stress of rats flown on STS-107” 2005-Soyuz Taxi flight: “Blood oxidative stress (BOS) on the Italian Astronaut R. Vittori “ 2007 Foton M3: LIFE Mission, TARSE EXPERIMENT 2009 MDS- Mice drawer system, "Effects of space flight on erythrocytes and oxidative stress of rodents" 2010 BIOKISS- TRADIKISS: TARDIgrades in Space 2008-2011: ESA-MARS 500 Selected Publications from the last 10 years: 1. Rizzo AM, Altiero T, Corsetto PA, Montorfano G, Guidetti R, Rebecchi L. Space Flight Effects on Antioxidant Molecules in Dry Tardigrades: The TARDIKISS Experiment. Biomed Res Int. 2015;2015:167642. 2. Mereghetti P, Corsetto PA, Cremona A, Rizzo AM, Doglia SM, Ami D. A Fourier transform infrared spectroscopy study of cell membrane domain modifications induced by docosahexaenoic acid. Biochim Biophys Acta. 2014 Oct;1840(10):3115-22. 3. Rizzo AM, Corsetto PA, Farina F, Montorfano G, Pani G, Battaglia C, Sancini G, Palestini P. Repeated intratracheal instillation of PM10 induces lipid reshaping in lung parenchyma and in extra-pulmonary tissues. PLoS One. 2014 Sep 26;9(9):e106855. 4. Degiorgio D, Corsetto PA, Rizzo AM, Colombo C, Seia M, Costantino L, Montorfano G, Tomaiuolo R, Bordo D, Sansanelli S, Li M, Tavian D, Rastaldi MP, Coviello DA (2014). Two ABCB4 point mutations of strategic NBD-motifs do not prevent protein targeting to the plasma membrane but promote MDR3 dysfunction. European Journal of Human Genetic 22(5):633 . 5. Gelsomino G, Corsetto PA, Campia I, Montorfano G, Kopecka J, Castella B, Gazzano E, Ghigo D, Rizzo AM and Riganti C. (2013). Omega 3 fatty acids chemosensitize multidrug resistant

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colon cancer cells by down-regulating cholesterol synthesis and altering detergent resistant membranes composition. Molecular Cancer 13;12:137. Mainini F, Contini A, Nava D, Corsetto PA, Rizzo AM, •Agradi E,• Pini E (2013). Synthesis, Molecular Characterization and Preliminary Antioxidant Activity Evaluation of Quercetin Fatty Esters. J Am. Oil. Chem Soc. DOI: 10.1007/s11746-013-2314-0. Monici M, Cialdai F, Romano G, Corsetto PA, Rizzo AM, Caselli A, Ranaldi F (2013). Effect of IR laser on myoblasts: Prospects of application for counteracting microgravity-induced muscle atrophy. Microgravity Science and Technology 25, 35:42. Rizzo AM, Corsetto PA, Montorfano G, Opizzi A, Faliva M, Giacosa A, Ricevuti G, Berra B, M Rondanelli (2012).Comparison between the AA/EPA ratio in depressed and non-depressed elderly females: omega-3 fatty acids supplementation correlates with improved symptoms but does not change immunological parameters. Nutrition Journal 11:82. Orsini F, Cremona A, Arosio P, Corsetto PA, Montorfano G, Lascialfari A, Rizzo AM (2012). Atomic force microscopy imaging of lipid rafts of human breast cancer cells. Biochim Biophys Acta. 1818(12):2943-2949. Guidetti R, Rizzo AM, Altiero T, Rebecchi L (2012). What can we learn from the toughest animals of the Earth? Water bears (tardigrades) as multicellular model organisms in order to perform scientific preparations for lunar exploration. Planetary and Space Science Corsetto PA, Cremona A, Montorfano G, Jovenitti IE, Orsini F, Arosio P, Rizzo AM (2012). Chemical-Physical Changes in Cell Membrane Microdomains of Breast Cancer Cells After Omega-3 PUFA Incorporation. Cell Biochem Biophys. 6: 45-59. Rizzo AM, Corsetto PA, Montorfano G, Milani S, Zava S, Tavella S, Cancedda R, Berra B (2012). Effects of long-term space flight on erythrocytes and oxidative stress of rodents. PLoS One. 7(3):e32361. IF 4.092 Rondanelli M, Giacosa A, Opizzi A, Pelucchi C, La Vecchia C, Montorfano G, Negroni M, Berra B, Politi P, Rizzo AM (2011). Long chain omega 3 polyunsaturated fatty acids Supplementation in the treatment of elderly depression: Effects on depressive symptoms, on phospholipids fatty acids Profile and on health-related quality of life. J Nutr Health Aging. 15(11):37-44. IF 1.712 Tedeschi G, Pagliato L, Negroni M, Montorfano G, Corsetto P, Nonnis S, Negri A, Rizzo AM (2011). Protein pattern of Xenopus laevis embryos grown in simulated microgravity. Cell Biol Int. 35:249-5. Rebecchi L, Altiero T, Cesari M, Bertolani R, Rizzo AM, Corsetto Paola, Guidetti R (2011). Resistance of the anhydrobiotic eutardigrade Paramacrobiotus richtersi to space flight (LIFE– TARSE mission on FOTON-M3)..Journal of Zoological Systematics and Evolutionary Research, 49:98-103. Rea G;, Antonacci A, Lambreva M, Pastorelli S, Tibuzzi A Ferrari S, Fischer D, Johanningmeier U, Oleszek W, Doroszewska T, Rizzo AM, Berselli P, Berra B, Bertoli A, Pistelli L, Ruffoni B, Blanchard CC, Marty JL, Litescu SC, Diaconu M, Touloupakis E, Ghanotakis D, Giardi MT (2011). Integrated plant biotechnologies applied to safer and healthier food production: the NutraSnack manufacturing chain. Trends in Food Science and Technology. 22: 353-366. Riganti C, Voena C, Kopecka A, Corsetto PA, Montorfano G, Enrico E, Costamagna C, Rizzo AM, Ghigo D, Dario, Bosia A (2011). Liposome-encapsulated doxorubicin reverses drug-resistance by inhibiting P-glycoprotein in human cancer cells. Molecular Pharmaceutics, 8: 683-700. Corsetto PA, Montorfano G, Zava S, Jovenitti IE, Cremona A, Berra B, Rizzo AM (2011). Effects of n-3 PUFAs on breast cancer cells through their incorporation in plasma membrane. Lipids Health Dis. 10(1) 73.

19. Rizzo AM, Montorfano G, Negroni M, Adorni L, Corsetto PA, Berselli P, Wahle K, Berra B (2010) A rapid method for determining arachidonic:eicosapentaenoic acid ratios in whole blood lipids: correlation with erythrocyte membrane ratios and validation in a large Italian population of various ages and pathologies. Lipids Health Dis. 9(1):7. 20. Rizzo AM, Negroni M, Altiero T, Montorfano G, Corsetto P, Berselli P, Berra B, Guidetti R, Rebecchi L (2010) . Antioxidant defences in hydrated and desiccated states of the tardigrade Paramacrobiotus richtersi. Comp Biochem Physiol B Biochem Mol Biol. 156(2):115-21. 21. Rondanelli M, Opizzi A, Giacosa A, Pelucchi C, Lavecchia C, Montorfano G, Negroni M, Berra B, Rizzo AM (2010). Effect of omega 3 Fatty Acids supplementation on health-related quality of life and on depressive symptoms in the treatment of elderly depression. A double blind placebo controlled randomized clinical trial. J Am Coll Nutr. 29(1):55-64. 22. Berselli P, Negroni M, Montorfano G, Corsetto, Krzyzanowska J, Oleszek W, Berra, B, Rizzo AM (2010). A Mint purified extract protects human keratinocytes from short-term, chemically induced oxidative stress. Journal of Agricultural and Food Chemistry, 58 (21):11428–11434. 23. Rizzo AM, Berselli P, Zava S, Montorfano G, Negroni M, Corsetto P, Berra B (2010). Endogenous antioxidants and radical scavengers. Adv Exp Med Biol. 698:52-67. 24. Berra B, Rizzo AM (2009). Melatonin: circadian rhythm regulator, chronobiotic, antioxidant and beyond. Clin Dermatol. 27(2):202-9. 25. Rebecchi L, Altiero T, Guidetti R, Cesari M, Bertolani R, Negroni M, Rizzo AM (2009) Tardigrade Resistance To Space Effects: First Results Of The Experiment Of Life – Tarse Mission On Foton-M3 (September 2007). Astrobiology. 9(6):581-91. 26. Rizzo AM, Montorfano G, Negroni M, Corsetto P, Berselli P, Marciani P, Zava S, Berra B (2009). Simulated microgravity induce glutathione antioxidant pathway in Xenopus laevis embryos. Cell Biol Int. 33(8):893-8. 28. Berra B, Rizzo AM (2009) . Glycemic Index, Glycemic Load: New Evidence for a Link with Acne. J Am Coll Nutr. 28: 450S-454S 29. Rizzo AM, Montorfano G, Adorni L, Berra B (2007). Antioxidant Metabolism of Xenopus laevis during the first days of development. Comp Biochem Physiol B Biochem Mol Biol. 146(1):94100. 30. Rizzo AM, Rossi F, Zava S, Montorfano G, Adorni L, Cotronei V, Zanini A, Berra B (2007) Antioxidant Metabolisms of Xenopus laevis embryos is affected by stratospheric balloon flight. Cell Biol Int. 2007 Jul;31(7):716-23. 31. Germano M, Meleleo D, Montorfano G, Adorni L, Negroni M, Berra B, Rizzo A M (2007) Plasma, red blood cells phospholipids and clinical evaluation after long chain omega-3 supplementation in children with attention deficit hyperactivity disorder (ADHD) Nutr Neurosci. 10(1-2):1-9 32. Rizzo AM, Montorfano G, Adorni L, Negroni M, Berra B. (2007). Blood and Oxidative Stress (BOS) during the Soyuz mission “ Eneide”. Microgravity science and Technology, XIX-2, 45-49. 33. Fontani G, Corradeschi A, Felici A, Slfatti F, Burgarini R, Fiaschi AI, Cerretani D, Montorfano G, Rizzo AM, Berra B (2005). Blood profiles, body fat and mood state in healthy subjects on different diets supplemented with omega-3 polyunsaturated fatty acids. Eur. J. Clin Invest. 35, 499-507.