Correction

BIOCHEMISTRY. For the article ‘‘Enzyme structure and dynamics

affect hydrogen tunneling: The impact of a remote side chain (I553) in soybean lipoxygenase-1,’’ by Matthew P. Meyer, Diana R. Tomchick, and Judith P. Klinman, which appeared in issue 4, January 29, 2008, of Proc Natl Acad Sci USA (105:1146–1151; first published January 23, 2008; 10.1073兾pnas.0710643105), the

authors note that in Table 1, the values given for the calculated Arrhenius parameters and the input parameters appeared incorrectly. The parameters were calculated incorrectly due to a sign inversion. Because the trends remain the same, this error does not affect the conclusions of the article. The corrected table appears below.

Table 1. Summary of empirical and computed Arrhenius parameters Calculated Arrhenius parameters*

Experimental Arrhenius parameters

Input parameters†

SLO mutant

Ea(H), kcal/mol

Ea(D) ⫺ Ea(H), kcal/mol

AH/AD

Ea(D) ⫺ Ea(H), kcal/mol

AH/AD

r0, Å

⍀Gating, cm⫺1

WT Ile553 3 Val Ile553 3 Leu Ile553 3 Ala Ile553 3 Gly

2.1 ⫾ 0.2‡ 2.4 ⫾ 0.5 0.4 ⫾ 0.7 1.9 ⫾ 0.2‡ 0.03 ⫾ 0.04

0.9 ⫾ 0.2‡ 2.6 ⫾ 0.5 3.4 ⫾ 0.6 4.0 ⫾ 0.3‡ 5.3 ⫾ 0.7

18 ⫾ 5‡ 0.3 ⫾ 0.2 0.3 ⫾ 0.4 0.12 ⫾ 0.06‡ 0.027 ⫾ 0.034

1.0§ 3.1 3.4 4.0§ 5.2

15§ 0.4 0.2 0.11§ 0.027

0.66§ 1.24 1.43 1.79§ 2.55

292§ 64 56 47§ 38

*Calculated by treating the hydrogen wave functions as Morse oscillators. The significance of these calculations is the trends in parameters, not their absolute magnitude. † r is the initial distance that hydrogen would have to transfer between the donor and acceptor (prior to gating). ⍀ 0 gating is the frequency of the gating mode. ‡Data from ref. 15. §Data from ref. 16.

CORRECTION

www.pnas.org兾cgi兾doi兾10.1073兾pnas.0809757105

www.pnas.org

PNAS 兩 December 9, 2008 兩 vol. 105 兩 no. 49 兩 19561

Correction

Jan 29, 2008 - AH/AD. Ea(D). Ea(H), kcal/mol. AH/AD r0, Å. Gating, cm 1. WT. 2.1. 0.2‡. 0.9. 0.2‡. 18. 5‡. 1.0§. 15§ ... ‡Data from ref. 15. §Data from ref. 16.

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