Client: Date: Sample: Notes about sample: ALL ASSESSMENTS DONE AT 400X TOTAL MAGNIFICATION Field # Dilution used: Bacterial #
1
2
3
4
5
12
16
20
25
6 25
TEST FOR YOUR
1 drop of sample, multiply by 20 drops in a ml
2400 = # fields at 400X in a 22 X 18 mm cover slip
particular dropper
2 drops, multiply by 10 drops in a ml
3600 = #fields at 400X in a 22 by 22 mm coverslip 7
8
9
10
11
12
13
14
15
16
17
18
19
20
Mean
StDev
22,5
6,5
WRITE IN DILUTION USED HERE AND IN X7 8
1600 = #of fields at 400X in at 18 X 18 mm coverslip
20
29
33
18
29
22
23
23
35
22
25
20
18
27
Dilution factor 1000
Mean Organisms per gram (mean*dil)*fields*#drops
Mean Micrograms/g or ml Org * biomass per org
1 620 000 000
3240 1 bacterium on average weighs 2 picograms
Actinobacteria
0
0,2
0,1
0
0,1
0,05
0,1
0,05
0,05
0,1
0,2
0,1
0,2
0,3
0,1
0,1
0,05
0,1
0,3
0,05
0,1125
0,09
5
Bacterial size/shape Dilution used for fungi:
Fungal Strands
WRITE IN DILUTION USED HERE AND IN X15 0,2
0
# drops = 1 drop of sample, multiply by 20 drops in a ml 2 drops, multiply by 10 drops in a ml Fungal strands / gram Micrograms of fungi per gm or ml
Mean # fungal strands
0,1
0,1
0,05
0
0,2
0,1
0,05
0,2
0,15
0,2
0
0,1
0,1
0,2
0,2
0,05
0,2
3
4
2,5
2
3
3
2
5
2-4
2-3
0
3
5
4
4
2
3
Color
Bc
B
B
C
B
B
C
B
C-B
C-B
0
B
B
Bf
Bf
C
Bf
Flag Am Ciliates
1337
Fields = MUST CHANGE 1600 to 2400 or 3600 in all equations to reflect size of cover slip
Diameters µm, color
Dilution used for protozoa:
40500
0
0,11
0,08
5
3,0
39600 (mean*dil)* fields*drops
627 (strands*mult fact)*gm/cm3 biomass
convert this to biomass based on: 1 fungal strand is assumed to be at least 50 um long. I cubic cm of fungal biomass = 0.33 g per cubic cm
WRITE IN DILUTION USED HERE AND IN X22, X23 and X24
Protozoa numbers per gram or ml
conv ag weeds annuals:; vegetables productive pasture: shrubs, decid forest conifer/old growth
3 3,5 4 4,5 5
0,000000035 0,000000048 0,000000063 0,000000079 0,000000098
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0,00
0,00
5
0
5,5
0,000000119
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0,00
0,00
5
0
6
0,000000141
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0,00
0,00
5
0
6,5 7 7,5 8
0,000000166 0,000000192 0,000000221 0,000000251
10,0
8,5
0,000000284
9
0,000000318
9,5 10
0,000000354 0,000000393
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
B
But then: Always require more than 300 ug per gram soil for healthy systems, unless weeds desired Must balance with fungi for the plant desired Less than 300 ug, will have trouble holding nutrients, will have poor soil structure, will have trouble protecting roots
Bacteria need to be properly balanced with fungi BASED ON THE PLANT DESIRED Protozoa and nematodes
0,000000016 0,000000025
0
0
0
0
0
0,2000
5
Since scan whole slide at 1:5 dilution, only have to mult by the portion of a ml examined
Bacteria should always be above 600 000 000
2 2,5
0
WRITE IN DILUTION USED HERE AND IN X28
B, F, P, R
Manually select mult factor from table based on average diameter--->
Mult factor
0
mean * dil * fidls * drops
Nematodes
Avg diameter
CILIATES SHOULD NOT BE PRESENT FOLLOWING IS FOR FLAGELLATES AND AMOEBAE no prots, no good nemas Less than 0.7 per field 1 to 1.5 per field IN SPRING 1.5 to 5 per field IN GROWING SEASON 3 or more per field through the growing season OR EITHER 1 to 2 protozoa per field OR
NEMATODES Root-feeders present Root-feeders typically present At least 1 bacterial feeder and possibly fungal feeders present 1 - 2 bacterial feeder and possibly fungal feeders present 1 to 5 bacterial-feeders, 1 to 3 fungal-feeders; 1 predatory nematode desirable 1 to 5 bacterial-feeders, 1 to 3 fungal-feeders; 1 predatory nematode desirable
