B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
3rd Semester
Second Year Engineering Subject Code
Category
Course Name
PBT3I101
PC
Biochemistry
PBT3I102
PC
Cell Biology
PBT3I103
PC
Biostatistics
PBT3I104
PC
Microbiology
PBT3I001
PC
PEK3E001 / POB3E002
HS
Upstream Process Engineering.-I Engineering Economics/ Organizational Behavior
PBT3D001 PBT3G001
Total Total Marks: 1100 Total Credits: 23 Honours
Genetics
Minor Specialization
Biostatistics
Theory
Third Semester
Hours/ week L/T
Credit Theory
University Marks
Internal Evaluation
3-0 3-0 3-0 3-0 3-1
3 3 3 3 4
100 100 100 100 100
50 50 50 50 50
2-1
3
100
50
19
19
600
300
4
4
100
50
Practical
Hours/ Week L/T
Credit Practical
Marks
2 2 2 2
1 1 1 1
50 50 50 50
8
4
200
Page
7
--Semester : 3rd -------------1. PBT3D001 Honours(CP) Genetics 4-0-0 4 2. PEK3E001 HS(O1) Engineering Economics 3-0-0 3 3. POB3E002 HS(O1) Organizational Behavior 3-0-0 3 4. PBT3G001 Minor(CP) Biostatics 4-0-0 4 5. PBT3I001 PC(CP) Upstream Process Engineering - I 4-0-0 4 6. PBT3I101 PC(CP) Biochemistry 3-0-1 4 7. PBT3I102 PC(CP) Cell Biology 3-0-1 4 8. PBT3I103 PC(CP) Biostatistics 3-0-1 4 9. PBT3I104 PC(CP) Microbiology 3-0-1 4
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
3rd Semester
3RD SEM PBT3I101 BIOCHEMISTRY Module-I Structure and Function of Carbohydrates: Monosaccharide, Oligosaccharides, Polysaccharides (Starch, Glycogen, Cellulose), Optical Isomerism Structure and Function of Lipids: Saturated and Unsaturated Fatty Acids, Triacylglycerols, Phosphoglycerides, Sphingolipids, Waxes and Sterol Structure and Function of Proteins: 20 Amino acids, Peptide bond, Hierarchy of protein architecture, Ramachandran Plot Structure and Function of Nucleic Acids: DNA, RNA, Double Helix Model of DNA, Denaturation and Renaturation DNA; Structure and function of Hormones, Minerals and Vitamins Module-II Principle of Bioenergetics: Bioenergetics and Thermodynamics, Phosphoryl group transfer and energy currency-ATP; Biological Oxidation and reduction reactions Metabolism-I: Introduction to metabolic processes; Metabolism of Carbohydrates: Glycolysis, TCA Cycle, ETS and Oxidative Phosphorylation, Gluconeogenesis , Metabolism of Lipids: Anabolism (Saturated), Catabolism (α- Oxidation, β-Oxidation) and Energetics of lipid metabolism; Metabolism Of Nucleic Acids: Catabolism and anabolism of purine and pyrimidine nucleotides. Photosynthesis: Light reaction and dark reaction. Module-III Metabolism-II: Metabolism of proteins: Biosynthesis of amino acids (role of precursors); Enzymes: Properties of Enzyme, Classification of Enzymes, Mechanism of enzyme action, Kinetics of enzyme action, Activation energy, Enzyme Inhibition, Coenzyme Text Book 1. Principle of Bio-Chemistry – Lehinger, Nelson and Cox 2. Biochemistry of Biochemistry by L. Stryer 3. Fundamentals of Biochemistry – Voet & Voet 4. Biochemistry by Zubay. 5. Biochemistry, C.B.Powar & G.R.Chatwal, Himalaya Publishing House. 6. Biochemistry, Rastogi, Tata McGraw Hill. 7. Fundamental of Biochemistry, Jain and Jain
Books: Introduction to Practical Biochemistry, Plummer, Tata McGraw Hill
8
Spectrophotometric/Colorimetric estimation of Protein using Lowry’s Method Spectrophotometric/Colorimetric estimation of carbohydrates Spectrophotometric estimation of DNA using DPA method Spectrophotometric estimation of RNA using Orcinol Method Estimation of iodine Number and Saponification value of fatty acids Separation of Amino acids by Paper Chromatography Separation of Sugars by Thin Layer Chromatography Separation of Proteins by Column Chromatography Assay of Enzyme activity: Protease from bacteria Assay of Enzyme activity: Amylase from Plant tissue & Saliva Determination of Km and Vmax of an enzyme catalyzed reaction.
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BIOCHEMISTRY LAB
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
3rd Semester
PBT3I102 CELL BIOLOGY
Module-I Structural intricacy & Chemical composition of cells, Organization of Cell (Prokaryotic and Eukaryotic), Cell Wall & Cell Membrane, Cell Organelles, Endoplasmic reticulum, Nucleus, Cytoskeleton
Module-II Molecular Organization of Chromosome (Nucleosome concept), Cell Cycle, Cell Divisions- Mitosis and Meiosis, Programmed cell death. introduction to cell fate and early embryonic patterning in different organisms (Drosophila), Stem Cell (Embryonic and adult types and characteristics )
Module-III Membrane transport & trafficking, mechanisms of protein sorting and targeting, intercellular communication and associated signaling pathways, cell death pathways, Cancer Cell Biology (Cause, Cell Characteristics) Text Books 1. Theory & Problems in Molecular & Cell Biology, Stansfield, Tata McGraw Hill 2. The Cell Molecular approach, Geoffrey M. Cooper, ASM press Washington D.C. Sinauer Associates, Inc. 3. Principles of Genetics, Robert Tamarin, Tata McGraw Hill 4. Molecular Biology of Cell – Alberts, Garland Science, Taylor & Francis Group.
5. Molecular Biology of The Cell (Fifth edition), 2007 by Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter. Publisher: Garland Publications 6. Molecular Cell Biology (sixth edition), 2008 by Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Matthew P. Scott, Anthony Bretscher, Hidde Ploegh, Paul Matsudaira. Publisher: W H Freeman & Company 7. Essential Cell Biology (Third Edition), 2009 by Bruce Alberts, Dennis Bray, Karen Hopkin, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter. Publisher: Garland Science
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CELL BIOLOGY LAB.
1. This Exercise focuses on how to develop a working knowledge of the Microscope and its use. Students should identify the different parts of the Microscope. List and follow recommended procedures in using and caring for the Microscope. 2. Mitosis and the Cell Cycle in Onion Root-Tip Cells 3. Meiotic cell division in grasshopper testis 4. Cell Counting and viability study 5. To isolate the mitochondria from the given sample 6. To identify the blood cell types in human blood smear. 7. To identify the different types cells present in the leaf cross section. 8. To prepare permanent slides using the given sections like Plant and animal samples. 9. Separation of lymphocytes and granulocytes from blood sample 10. To study cellular fractionation of a homogenized rat liver via a technique called differential centrifugation. 11. To study the technical principles underlying Scanning Electron Microscopy (SEM).
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
PBT3I104
3rd Semester
MICRO BIOLOGY
Module-I Introduction to Microbial Kingdom- Bacteria, Viruses, Fungi and Yeast; Classical and Modern approaches of microbial taxonomy; Classification of bacteria, fungi and Viruses; Methods of Microbiology- Culture media, Sterilization, Establishment of pure culture, Staining of bacteria (Gram’s, Acid Fast, Capsule), Micrometry and Microscopy(Bright Field, Fluorescence, Phase Contrast and Electron). Module-II
Microbial growth and metabolism: Pattern of bacterial growth, Growth kinetics, Monod’s Equation, Synchronous Growth and its Kinetics, Continuous culture and its growth kinetics, Growth inhibitory substances. Metabolism of carbohydrate in bacteria, Enerdoudorf,s pathway and glyoxalate pathway, Energy transduction mechanism in bacteria, Cyanobacteria and nitrogen fixation, Anaerobic respiration. Microbial genetics: Organization of bacterial and viral genome, Plasmids and Episomes, Genetic recombination in bacteria (Transformation, Conjugation and Transduction), Genetic analysis in bacteria, DNA repair mechanisms in bacteria, Transposons, Mutation in Microorganisms. Module-III
Food Microbiology: Microbiology of foods, Types of microbes associated with food spoilage, Food preservation methods, Food poisioning, Microbiology of Milk and dairy products. Medical Microbiology: disease causing bacteria, virus and fungi; Antimicrobial agents, Antibiotics, Disinfectants and Vaccines Environmental Microbiology: Microbiology of water, Microbiology of Air, Baceriological analysis of water & water treatment, Microbiology of extreme environments (Halobacteria, Methanogens, Thermofiles), Microbiology of sewage. Text Books : 1. Text book of Microbiology by Stanier. 2. Microbiology by Pelczar 3. Brock Biology of micro-organisms 4. Microbiology by Presscott. 5. Microbial Genetics- Freifelder 6. Microbiology and Immunology by B K Patnaik, T.C. Kar, H.N. Thatoi, India-Tech publication. New Delhi Micrometry: calibration of stage and ocular micrometer and measurement of microbial sample. Staining of microbial sample ( Gram’s Staining, Capsule staining, Fungal staining) Media preparation and sterilization (Slant, Stab and Broth culture) Isolation of micro organisms from natural habitats (Air, Water, Soil & Milk) Establishment of pure culture by streak plate and serial dilution method. Study the bacterial growth curve using spectrophotometer and viability assessment. Antibiotic assay and estimation of Zone of inhibition. Chemical assay and MIC determination of antibiotics. Biochemical assay of microorganisms (Starch Hydrolysis, Casein Hydrolysis and IMVIC test). Microscopy: Study of Compound, Phase contrast and Fluorescence Microscopes.
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MICRO BIOLOGY LAB
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
3rd Semester
MOLECULAR BIOLOGY Module-I Genome Organization Prokayotes and Eukaryotes, Nuclear genome and Organellar genome, DNA as the genetic material, Central dogma of molecular biology, Genome complexity, C- value Paradox, Cot curve analysis, Repetitive DNA, satellite DNA; Gene structure in prokaryotes and Eukaryotes, Cistron, Recon, Muton; Variants of gene- Split genes, pseudogenes, Overlapping genes and selfish DNA. DNA Replication: Models of DNA replication, Enzymology of DNA replication, Process of DNA replication (Initiation, Elongation, Termination), DNA replication at the telomere, Replication of Mitochondrial and Chloroplast genome, DNA-protein interaction, DNA repair. Module-II Transcription: Components of transcription machinery in prokaryotes and eukaryotes, Transcription factors, Transcription process (Initiation, Elongation, Termination), Transcription factors, m-RNA processing, Pre and Post transcriptional processing, Capping and poly (A) tailing, m-RNA stability, m-RNA editing, Gene Silencing
Module-III Translation: Genetic code- the principle of translation, Translation machinery (t-RNA, Aminoacyl synthetase, Ribosome), Translation process, Post translational modification of protein. Regulation of Gene Expression: Constitutive and Induced gene expression, Regulation of gene expression in prokaryotes and eukaryotes, Operon model (Lac-operon and Trp- operon), DNA methylation. Molecular evolution (DNA based phylogenetic trees and its applications), Introduction to recombinant DNA technology. Books :
Text Book of Molecular Biology By Padmanabhan and Shastri. MOLBIO – Avinash & K. Upadhyaya, Himalaya Publishing House. Mol. Biology. by Turner. Mol. “Biology of Gene” – Watson Principles of Mol. Biology - OS Prim Rose Recombinant DNA Technology – Watson Mol. Cell Biology. - Baltimore
1. Isolation, purification of DNA from plant sample and its yield estimation. 2. Isolation, purification of DNA from blood sample and its quantification using UV spectrophotometer. 3. Isolation, purification of DNA from bacterial sample and its quality assessment using UV spectrophotometry. 4. Isolation of plasmid DNA and estimation its size using agarose gel electrophoresis. 5. Effect of gel concentration on solidification and migration of DNA sample 6. Restriction digestion of supplied DNA sample and estimate the molecular weight of the fragments resulted. 7. Elution of the DNA from the supplied gel and assess the integrity of the fragments. 8. Isolation and purification of RNA from plant/bacterial sample and its quantification using UV spectrophotometer. 9. Isolation and purification of protein from the supplied sample and its quantification using UV spectrophotometer.
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MOLECULAR BIOLOGY LAB
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B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
3rd Semester
PBT3I103 BIOSTATISTICS
Module-I: Introduction and definition of Biostatistics; Concept of variables in biological systems. Collection, Classification, tabulation graphical and diagrammatic representation of numerical data; Measures of central tendency: Mean, Median and Mode and their relationship; Measures of dispersion: Range, Quartile deviation, Mean deviation, Standard deviation, Concept of standard error, Coefficient of variation, Skew ness and Kurtosis.
Module-II: Probability: Random experiment, events, sample space, mutually exclusive events, independent and dependent events; Various definitions of probability, addition and multiplication theorems of probability, Random variables (discrete and continuous), Probability density functions and its properties; Probability distributions: normal, Binomial, Poisson and their application.
Module-III: Concept of populations and sample. Simple random sampling without replacement. Definition of simple random sample; Designing of Experiments-Random block design and Split plot design; Correlation and Regression, linear and quadratic regression; Analysis of variance: One- way and two-way classifications with single observation per cell. Duncan’s multiple range test; Tests of significance: Chi-square, student’s t, z and f-distributions, their properties and uses. Text Books: 1. Biostatistics: Rao KS, Himalaya Publishing House 2. Introduction to Biostatistics & Research Methods: Sundar Rao PSS & Richard J, PHI learning Pvt. Ltd. 3. Biostatistics: Arora and Mohan, Himalaya Publishing House
BIOSTATISTICS LAB
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12
The practicals to be conducted with available sample data set. 1. Introduction to biostatistics and measurement. 2. Construct and interpret graphical displays such as histograms, box plots, bar charts, stem & leaf plots, and bivariate scatterplots 3. Collection of sample data and opening sample datasets 4. Calculating measures of central tendency and dispersion. 5. Hypothesis testing; Define null and research hypotheses. Practice conducting and interpreting T-test in Stata and interpreting confidence intervals 6. Hypothesis testing; Define null and research hypotheses. Practice conducting and interpreting ANOVA and interpreting confidence intervals 7. Conduct and interpret correlation 8. Conduct and interpret linear regression 9. Conduct and interpret multiple linear regression 10. To calculate and interpret binomial probabilities and Normal probabilities
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
3rd Semester
PBT3I001 UPSTREAM PROCESS ENGINEERING-I Module-I: Fluid Mechanics concept ( 14 lectures) Physical properties of Fluid: Density, specific gravity, specific weight, specific volume, surface tension and capillarity, viscosity, compressibility and bulk modulus, fluid classification. Fluid static pressure, Pascal’s, pressure variation for incompressible fluid, atmospheric pressure, absolute pressure, gauge pressure and vacuum pressure, manometer. Hydrostatic pressure on submerged surface, force on a horizontal submerged plane surface, force on a vertical submerged plane surface. Buoyancy and flotation, Archimedes’ principle, stability of immersed and floating bodies, determination of metacentric height. Module –II: Elements of fluid dynamics ( 12 lectures ) Basic equations of fluid flow: Newtonian and non-Newtonian fluids, turbulence and its nature, Reynolds number and transition from laminar to turbulent flow, boundary layer flow, continuity equation, Bernoulli’s equation, pump workin Bernoulli’s equation. Flow of incompressible fluids: Fluid flow in pipes, friction factor, laminar flow in pipes, HagenPoiseuille equation, effect of roughness, friction factor charts, Reynolds number and friction factor relationship Flow through packed beds: Ergun, Kozney- Carman and Blake-Plummer equations, principles of two-phase and three-phase fluidizaion. Module-III : Transportation and metering of fluids ( 10 lectures ) Pipe fittings and valves, positive displacement pumps, power and efficiency of pumps, centrifugal, plunger and piston pumps, blower and compressor, flow measuring devices viz. venture meter, orifice meter, pitot tube and rotameter. Drag force and terminal settling velocity, principles of solid-liquid separation- filters and centrifuges.
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13
Text Books 1. McCabe, Smith and Harriot, Unit Operations of Chemical Engineering 2. Foust et al, Principles of Unit Operations. 3. Treybal, R.E. Mass Transfer Operations 4. Badger and Banchero. Introduction to Chemical Engineering. 5. Foust, Wenzel, Clump, Maus and Andersen, Principles of Unit Operations. 6. Geankoplis, Transport processes and unit operations
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
Module-II
PBT3D001
3rd Semester
GENETICS
Principles of Inheritance: Chromosome theory of Heredity, Mendelism, Non-Mendelian Gene Interactions (Epistasis, Lethality, Pleiotropy), Allelic Complementation, Cytoplasmic Inheritance,
Module-II
Linkage and Crossing over, Chromosome mapping, Mutation and Chromosomal Aberration, Transposable genetic elements, Heterosis and Hybrid vigour, Inbreeding depressions, Genetic diseases in Human ( Colour blindness, Haemophillia).
Module-III
Quantitative Inheritance: Polygenes and Multiple alleles, Introduction to QTLs and its inheritance, Detection of QTLs Principles breeding in plants and animals Population Genetics: Hardy-Weinberg’s law, Genetic Equilibrium, Changes in gene frequency, Genetic Drift, Effect of evolutionary forces on genetic equilibrium of apopulation. Developmental genetic with reference to Arabidopsis and Drosophilla.
Text Books
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1. Theory & Problems in Molecular & Cell Biology, Stansfield, Tata McGraw Hill 2. The Cell Molecular approach, Geoffrey M. Cooper, ASM press Washington D.C. Sinauer Associates, Inc. 3. Principles of Genetics, Robert Tamarin, Tata McGraw Hill 4. Molecular Biology of Cell – Alberts, Garland Science, Taylor & Francis Group. 5. Concept of Genetics by Klug. 6. Genetics, David R Hyde, Tata McGraw Hill.
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
PEK3E001
3rd Semester
ENGINEERING ECONOMICS
Theory L/T (Hours per week):2/1, Credit: 3
Module I (12 hours)
Engineering Economics- Nature, Scope, Basic problems of an economy, Micro Economics and Macro Economics. Demand- Meaning of demand, Demand function, Law of Demand and its exceptions, Determinants of demand, Elasticity of demand & its measurement (Simple numerical problems to be solved ), Supply-Meaning of supply, Law of supply and its exception, Determinants of supply, Elasticity of supply, Determination of market equilibrium (Simple numerical problems to be solved). Production-Production function, Laws of returns: Law of variable proportion, Law of returns to scale
Module II (12 hours)
Cost and revenue concepts, Basic understanding of different market structures, Determination of equilibrium price under perfect competition (Simple numerical problems to be solved), Break Even Analysis-linear approach (Simple numerical problems to be solved). Banking -Commercial bank, Functions of commercial bank, Central bank, Functions of Central Bank. Inflation-Meaning of inflation, types, causes, measures to control inflation. National Income-Definition, Concepts of national income, Method of measuring national income.
Module III (12 hours)
Time value of money- Interest - Simple and compound, nominal and effective rate of interest, Cash flow diagrams, Principles of economic equivalence. Evaluation of engineering projects-Present worth method, Future worth method, Annual worth method, Internal rate of return method, Cost benefit analysis for public projects . Depreciation- Depreciation of capital assert, Causes of depreciation, Methods of calculating depreciation (Straight line method, Declining balance method), After tax comparison of project.
Text Books
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15
1. Riggs, Bedworth and Randhwa, “Engineering Economics”, McGraw Hill Education India 2. Principles of Economics, Deviga Vengedasalam; Karunagaran Madhavan, Oxford University Press. 3. Engineering Economy by William G.Sullivan, Elin M.Wicks, C. Patric Koelling, Pearson 4. R.Paneer Seelvan, “ Engineering Economics”, PHI 5. Ahuja,H.L., “Principles of Micro Economics” , S.Chand & Company Ltd 6. Jhingan,M.L., “Macro Economic Theory” 7. Macro Economics by S.P.Gupta, TMH
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
3rd Semester
POB3E002 ORGANIZATIONAL BEHAVIOUR Credit- 3 Class Hours - 40 Objectives: 1. To develop an understanding of the behavior of individuals and groups inside organizations 2. To enhance skills in understanding and appreciating individuals, interpersonal, and group process for increased effectiveness both within and outside of organizations. 3. To develop theoretical and practical insights and problem-solving capabilities for effectively managing the organizational processes.
02
03
Class Hours Fundamentals of OB: Definition, scope and importance of OB, 6 Relationship between OB and the individual, Evolution of OB, Theoretical framework (cognitive), behavioristic and social cognitive), Limitations of OB.
Attitude: Importance of attitude in an organization, Right Attitude, Components of attitude, Relationship between behavior and attitude, Developing Emotional intelligence at the workplace, Job attitude, Barriers to changing attitudes. Personality and values: Definition and importance of Personality for performance, The Myers-Briggs Type Indicator and The Big Five personality model, Significant personality traits suitable to the workplace (personality and job – fit theory), Personality Tests and their practical applications.
Perception: Meaning and concept of perception, Factors influencing perception, Selective perception, Attribution theory, Perceptual process, Social perception (stereotyping and halo effect). Motivation: Definition & Concept of Motive & Motivation, The Content Theories of Motivation (Maslow’s Need Hierarchy & Herzberg’s Two Factor model Theory), The Process Theories (Vroom’s expectancy Theory & Porter Lawler model), Contemporary Theories – Equity Theory of Work Motivation. Foundations of Group Behavior: The Meaning of Group & Group behavior & Group Dynamics, Types of Groups, The Five – Stage Model of Group Development. Managing Teams: Why Work Teams, Work Teams in Organization, Developing Work Teams, Team Effectiveness & Team Building. Leadership: Concept of Leadership, Styles of Leadership, Trait Approach Contingency Leadership Approach, Contemporary leadership, Meaning and significance of contemporary leadership, Concept of transformations leadership, Contemporary theories of leadership, Success stories of today’s Global and Indian leaders.
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Contents
Page
Unit
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16 04
05
3rd Semester
Organizational Culture : Meaning & Definition of Organizational Culture, creating & Sustaining Organizational Culture, Types of Culture (Strong vs. Weak Culture, Soft Vs. Hard Culture & Formal vs. Informal Culture), Creating Positive Organizational Culture, Concept of Workplace Spirituality. Organizational Change: Meaning, Definition & Nature of Organizational Change, Types of Organizational Change, Forces that acts as stimulants to change. Implementing Organizational Change : How to overcome the Resistance to Change, Approaches to managing Organizational Change, Kurt Lewin’s-Three step model, Seven Stage model of Change & Kotter’s Eight-Step plan for Implementing Change, Leading the Change Process, Facilitating Change, Dealing with Individual & Group Resistance, Intervention Strategies for Facilitating Organizational Change, Methods of Implementing Organizational Change, Developing a Learning Organization.
8
7
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Reference Books 1. Understanding Organizational Behaviour, Parek, Oxford 2. Organizational Behaviour, Robbins, Judge, Sanghi, Pearson. 3. Organizational Behaviour, K. Awathappa,HPH. 4. Organizational Behaviour, VSP Rao, Excel 5. Introduction to Organizational Behaviour, Moorhead, Griffin, Cengage. 6. Organizational Behaviour, Hitt, Miller, Colella, Wiley
Course Name
HS
Purely Applied Mathematics for Specific Branch of Engineering Molecular Biology
PC PC PC PC HS
Upstream Process Engineering.-II Bioinstrumentation Biochemical Reaction Engineering Engineering Economics/ Organizational Behavior *Skill Project and Hands on
Total Total Marks: 1200 Total Credits: 25 Honours
Biomolecules And Cell Signaling
Minor
BioInstrumentation/ Bio-Chemical Reaction Engineering
Hours/ week L/T
Credit Theory
University Marks
Internal Evaluation
3-0
3
100
50
3-0 3-0
3 3
100 100
3-0 3-0
3 3
2-1
3
Practical
Hours/ Week L/T
Credit Practical
Marks
50 50
2 2
1 1
50 50
100 100
50 50
2 2
1 1
50 50
100
50 6
3
100
14
7
300
18
18
600
300
4
4
100
50
SEMESTER : 4TH -------------1. PBT4D001 HONOURS(CP) BIO-MOLECULES AND CELL SIGNALING 2. PBT4E001 HS(CP) PURELY APPLIED MATHEMATICS FOR SPECIFIC BRANCH OF ENGINEERING 3. PEK4E002 HS(O1) ENGINEERING ECONOMICS 4. POB4E003 HS(O1) ORGANIZATIONAL BEHAVIOR 5. PBT4G001 MINOR(O3) BIO-INSTRUMENTATION 6. PBT4G002 MINOR(O3) BIO-CHEMICAL REACTION ENGINEERING 7. PBT4I101 PC(CP) MOLECULAR BIOLOGY 8. PBT4I102 PC(CP) UPSTREAM PROCESS ENGINEERING - II 9. PBT4I103 PC(CP) BIOINSTRUMENTATION 10. PBT4I104 PC(CP) BIOCHEMICAL REACTION ENGINEERING 11. PBT4I201 PC(CP) SKILL PROJECT AND HANDS ON
4-0-0 3-0-0
4 3
3-0-0 3-0-0 4-0-0 4-0-0 3-0-1 3-0-1 3-0-1 3-0-1 0-0-3
3 3 4 4 4 4 4 4 3 --29 ---
18
Code
Fourth Semester Theory
Semester
Page
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16 4th
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16 4th
Module-I
PBT4I101 MOLECULAR BIOLOGY
Semester 4TH SEM
Genome Organization Prokayotes and Eukaryotes, Nuclear genome and Organellar genome, DNA as the genetic material, Central dogma of molecular biology, Genome complexity, C- value Paradox, Cot curve analysis, Gene structure in prokaryotes and Eukaryotes, Split genes, pseudogenes, Overlapping genes and selfish DNA
Module-II
DNA Replication: Models of DNA replication, Enzymology of DNA replication, Process of DNA replication (Initiation, Elongation, Termination), DNA replication at the telomere, Replication of Mitochondrial and Chloroplast genome, DNA-protein interaction, Mutagenesis, DNA repair, Regulation of DNA Replication.
Module-III
Transcription: Components of transcription machinery in prokaryotes and eukaryotes, Transcription process (Initiation, Elongation, Termination), Post transcriptional processing, mRNA editing, Gene Silencing Translation: Genetic code, Translation machinery (t-RNA, Aminoacyl synthetase, Ribosome), Translation process, Post translational modification of protein. Regulation of Gene Expression: Constitutive and Induced gene expression, Regulation of gene expression in prokaryotes and eukaryotes, Operon model (Lac-operon and Trp- operon), DNA methylation.
Books :
1. David Friefelder - Molecular Biology, 2nd Ed., Norasa Publishing Home 1987. 2. Lodish & Baltimore, Molecular Cell Biology, 5th Ed., W.H. Freeman & Company, 2003. 3. Gerald Karp - Cell and Molecular Biology, Concepts andExperiments, 5 th Edition, John Wiley and Sons Pvt. Ltd., 2008. 4. Benjamin Lewin - Gene – VIII, 1 st Edition,2004 5. Channarayappa - Molecular BiotechnologyPrinciples and Practices, 1 st Edition, 2006. University Press.
PBT4I102
UPSTREAM PROCESS ENGINEERING-II
Module-I:Processing of particulates
Properties and handling of particulate solids, size reduction equipments- working principles of crusher, grinder and pulverizer, screening and particle size distribution.
Module-II : Heat Transfer concepts and applications
Heat transfer and drop wise condensation, Heat transfer to boiling liquid, working principles of different types of heat transfer equipments like heat exchanger, condenser, evaporator and autoclave.
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Diffusion concepts: adsorption equillibria., working principles of mass transfer equipments like adsorber, absorber, distillation column and humidifier. Text Books 1. McCabe, Smith and Harriot, Unit Operations of Chemical Engineering 2. Foust et al, Principles of Unit Operations. 3. Treybal, R.E. Mass Transfer Operations 4. Badger and Banchero. Introduction to Chemical Engineering. 5. Foust, Wenzel, Clump, Maus and Andersen, Principles of Unit Operations.
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Module-III: Mass transfer concepts and applications
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16 4th
Module – I
PBT4I103
Semester
BIOINSTRUMENTATION
Spectroscopic methods to study physicochemical properties of Biomolecules: UV-Vis, IR, CD & ORD, DSC, FTIR Fluorescence & phosphorescence. Electrophoresis: General principle of electrophoresis, support media (agarose and polyacrylamide gels), electrophoresis of proteins by SDS-PAGE, native PAGE, gradient gels, isoelectric focusing, two dimensional PAGE, Blotting Techniques: Western, Southern, Northern blot analysis, visualization of proteins in gels, electrophoresis of nucleic acids using agarose gel.
Module – II
Brief introduction to NMR spectroscopy, hydrogen-deuterium exchange Mass spectroscopy and X-ray crystallography and their application. Hydrodynamic properties–viscosity. Principles of electron microscopy, preparation of samples, TEM, SEM Radioisotope Techniques: Radioactivity activity detection methods based on ionization (GeigerMuller monitor), excitation (solid and liquid scintillation counting), Cerenkov counting, autoradiography, safety aspects of handling radioactive material
Module – III
Chromatography: Principles of chromatography, distribution coefficient, retention time, capacity factor, plate height and resolution, peak broadening and van Deemter plot, TLC and column chromatography, matrix materials, LPLC, HPLC, normal phase and reversed phase chromatography, ion exchange chromatography, gel exclusion chromatography, affinity chromatography, Gas Chromatography Biosensors: Immobilization key to biosensor construction, Types of Biosensor, whole cell biosensors. Applications and uses of biosensors, Clinical chemistry, medicine and health care, Veterinary, Agriculture and Food production, Environmental control and pollution monitoring.
Books:
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1. Principles and Techniques of Biochemistry and Molecular Biology, Wilson K. and Walker J., Cambridge University Press (2005) 6th ed. 2. Biochemical Method-A Concise guide for students and researchers, Pingoud A., Urbanke C., Hoggett J. and Jeltsch A. Wiley-VCH Publishers 3. Bioseparations: Science and Engineering, Harrison, R.G., Todd, P., Rudge, S.R. and Petrides, B.B. Oxford University Press (2006). 4. Molecular Spectroscopy, McHale, J.L., Prentice Hall (1998). 5. Microscopy and Microtechniques. Marimuthu, R., MJP Publishers (2008). 6. Instrument Methods of Analysis, Willard H.W., Merritt L.L., Dean J.A. & Settle F.A. 6th ed. East West Publishers, 7. Turner, A.P.F., Karube.I.,and Wilson, G.S. Biosensors Fundamentals and applications, Oxford Univ.Press.
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16 4th
PBT4I104
Semester
BIOCHEMICAL REACTION ENGINEERING
Module I: Material, energy balance and concepts of reaction engineering
Material Balance & Energy Balances: Mathematical requisites – use of log-log and semi-log graph paper, triangular diagram, graphical differentiation and graphical integration, material balance without chemical reaction, material balance with chemical reaction, energy balance; enthalpy changes, heat of reaction and its temperature dependence, heats of solution and mixing, adiabatic flame temperature, use of psychometric charts. Kinetics of homogeneous reactions: classification of reactions, reaction rate, speed of reaction, rate equation, concentration-dependent term of rate equation, rate constant, order and molecularity, representation of elementary and nonelementary reactions, kinetic models for nonelementary reactions, temperature-dependent term of a rate equation, activation energy and temperature dependency.
Module II: Reaction engineering of batch and continuous reactors
Kinetic analysis of batch reactor data: Integral and differential methods for analyzing kinetic data, interpretation of constant volume batch reactor, data for zero, first, second and third order reactions, half life period, irreversible reaction in parallel and series, auto catalytic reaction. Kinetic interpretation of batch reactor data for single reactions: interpretation of variable volume batch reaction data for zero, first and second order reactions, Ideal batch reactor, steady state CSTR and plug flow reactors and their use for kinetic interpretation. Design for single reaction: size comparison of single reactors, plug flow reaction in series and/or parallel, equal and different size of mixed reactor in series, finding the best system for given conversion, recycle reactor, Design of multiple reactions in batch, CSTR and PFR.
Module III: Heterogeneous reactions and Biochemical reactions
Reaction catalyzed by solids: introduction to heterogeneous reactions, rate equation for surface kinetics, pore diffusion resistance combined with surface kinetics, porous catalyst particles, performance equations for reactors containing porous catalyst particles, experimental methods for finding rates, advantages and disadvantages of packed bed and fluidized bed catalytic reactors. Biochemical reaction systems: microbial fermentation, batch fermentor and mixed flow fermentor, kinetic expressions of fermentation.
Books
Page
21
1. Chemical process Principles (Part I & II), Houge, Watson & Ragatz, Asian Student Edition, Asia Publishing House. 2. Basic Principles and Calculations in Chemical Engineering, Himmelbalu, Prentice Hall (l) 6th Ed. 3. Coulson & Richardson’s Chemical Engineering- Volume 3 (Chemical and Biochemical Reactors and process controls) ed. Ricchardson. J.F., Peacock. D.G.,First Indian ed. Asian Books Pvt.Ltd. 1998. 4. Levenspiel, O., Chemical Reaction Engineering,Wiley Eastern Ltd. 5. Smith & Vanness, Thermodynamics for Chemical Engineers, MGH. 6. Bailey & Oils, Biochemical Engg. Fundamentals, MGH 7. Foggler, Elements of Chemical Reaction Engineering
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16 4th
Semester
MOLECULAR BIOLOGY LAB
1. Isolation, purification of DNA from plant sample and its yield estimation. 2. Isolation, purification of DNA from blood sample and its quantification using UV spectrophotometer. 3. Isolation, purification of DNA from bacterial sample and its quality assessment using UV spectrophotometry. 4. Isolation of plasmid DNA and estimation its size using agarose gel electrophoresis. 5. Effect of gel concentration on solidification and migration of DNA sample 6. Restriction digestion of supplied DNA sample and estimate the molecular weight of the fragments resulted. 7. Elution of the DNA from the supplied gel and assess the integrity of the fragments. 8. Isolation and purification of RNA from plant/bacterial sample and its quantification using UV spectrophotometer.. 9. Isolation and purification of protein from the supplied sample and its quantification using UV spectrophotometer.
UPSTREAM PROCESS ENGG LAB
Page
22
1. Experiments on Reynold’s Apparatus-Determination of flow regime and construction of friction factor against NRe. 2. Experiments on flow measuring devices - in closed conduit using (a) Venturimeter, (b) Orifice meter (c) Rotameter. 3. Study and verification of conservation of energy of a flowing liquid in a Bernoulli’s apparatus. 4. Determination of Pressure drop for flow through packed bed & verification of Ergun Equation, Kozeny-Karman equation, Blake-Plummer Equation. 5. To Determine the Overall heat transfer coefficient of a concentric pipe heat exchanger based on the inside diameter of the tube. 6. To calculate the heat loss in a lagged pipe made of various insulating materials. 7. Determination of volumetric mass transfer coefficient (Kla) of gas-liquid system. 8. Determination of mixing time in stirred tank reactor. 9. To determine the coefficient of absorption/adsorption in packed bed columns. 10. To separate the solute from one phase to another (aqueous to solvent) phase by liquidliquid extraction. 11. Double Pipe Heat Exchanger. 12. Shell and Tube Heat Exchanger.
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16 4th
Semester
BIOINSTRUMENTATION LAB
1. UV-Visible spectroscopy: UV – spectrophotometric analysis of DNA and protein samples/ Determine λmax of DNA, protein, bromophenol blue solutions by wavelength scan 2. Determination of secondary structure of protein by Circular dichroism (CD) spectroscopy. 3. Denaturation of proteins and nucleic acids. 4. Determination of Protein- ligand interaction. 5. Column chromatographic analysis of chlorophyll 6. 2D-TLC analysis of amino acids 7. Use of viscometer in protein analysis 8. Comparison of Coomassie brilliant blue and silver staining methods for visualizing protein bands in SDS-PAGE 9. Comparison of ethidium bromide and silver staining methods for visualisation of small DNA fragments analyzed by native PAGE 10. Fluorescence spectroscopy 11. GC & HPLC (demonstration) 12. Differential scanning calorimetry (DSC) 13. FTIR 14. Electron microscopy (SEM/TEM)
BIOCHEMICAL REACTION ENGINEERING LAB
1. Use of log-log and semi-log graph paper, triangular diagram, graphical differentiation and graphical integration 2. Kinetic Studies in C.S.T.R 3. Kinetic Studies in P.F.R 4. Kinetic Studies in Combined Reactor 5. Kinetic Studies in Batch Reactor 6. Adiabatic Batch Reactor 7. R.T.D Studies in C.S.T.R 8. R T D Studies in C.S.T.R’s in Series R.T.D Studies in Plug Flow Reactor 9. R.T.D Studies in Combined Reactor. TEXT BOOKS:
Page
23
1. Octave Levenspiel - Chemical Reaction Engineering , 3rd Ed. John Wiely & Sons,1999. 2. H.S. Fogler - Elements of Chemical Reaction Engineering, 2nd ed. PHI, 1992.
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16 4th
Semester
PBT4D001 HONORS: BIOLOGICAL MACROMOLECULES AND INTERACTION
Module-1
Introduction to biological macromolecules: General principles, Molecular interactions in macromolecular structures. Equilibrium binding: Boltzmann distributions and energy levels. Ligand-protein interactions, protein-protein interactions. Binding polynomials and partition functions; linked functions; coupled equilibriums. Phenomenological binding models vs. rigorous models of allosteric regulation and site-site cooperativity. Practical considerations of binding analysis; nonlinear least squares analysis. ITC, DSC and SPR methods .
Module-II
Nucleic acids: Special properties of nucleic acids; counterion condensation theory; effect of salt concentration and type on nucleic acid structure and nucleic acid-protein interactions. RNA structure and folding. Single-molecule nucleic acid biophysics.
Module-III
Single molecule science: Mechanisms of protein-ligand (nucleic acid) and protein-protein interactions; dynamics of intramolecular structural changes; single-molecule FRET; singlemolecule force spectroscopy monitored by laser optical tweezers and atomic force microscopy.
Books
1. Thermodynamic Theory of Site-Specific Binding Processes in Biological Macromolecules, DiCera (Cambridge University Press, 1995); 2. Molecular Driving Forces:Statistical Thermodynamics in Chemistry and Biology, Dill and Bromberg (Garland Sciences, 2003).
Suggested Readings / Books:
Page
24
1. Molecular Biophysics by Daune, M, Oxford University Press (1999). 2. Biophysics by Glaser, R, Springer (2004). 3. Lehninger’s Principles of Biochemistry by Nelson, D.L., Cox, M.M., McMillan Publishers (2008) 4th ed.
Course Name
PC PC
Genetic Engineering Plant & Animal Biotechnology Industrial Microbiology & Enzyme Technology Immunology &Immunotechnology DBMS/Process Instrumentation Advance Lab-I
PC PE OE
Theory
Fifth Semester
Hours/W eek L/T 3-0 3-0
Credit Theory
University Marks
Internal Evaluation
3 3
100 100
3-0
3
3-1 3-1
Practical
Credit Practical
Marks
50 50
Hours/ Week L/T 2 2
1 1
50 50
100
50
2
1
50
4
100
50
4
100
50 4 7
200 350
PC 8 Total 17 17 500 250 14 Total Marks: 1100 Total Credits: 24 Honours Genomics, 4 4 100 50 Proteomics And Metabolomics Minor Industrial Specialization Micro-Biology and Enzyme Technology SEMESTER : 5TH -------------1. PBT5D001 HONOURS(CP) GENOMICS, PROTEOMICS AND META-BOLOMICS 2. PBT5G001 MINOR(CP) INDUSTRIAL MICRO-BIOLOGY AND ENGYME TECHNOLOGY 3. PBT5H001 OE(O2) DBMS 4. PBT5H002 OE(O2) PROCESS INSTRUMENTATION 5. PBT5I101 PC(CP) GENETIC ENGINEERING 6. PBT5I102 PC(CP) PLANT & ANIMAL BIOTECHNOLOGY 7. PBT5I103 PC(CP) INDUSTRIAL MICROBIOLOGY & ENZYME TECHNOLOGY 8. PBT5I201 PC(CP) ADVANCE LAB - I 9. PBT5J001 PE(CP) IMMUNOLOGY &IMMUNOTECHNOLOGY
4-0-0 4-0-0
4 4
4-0-0 4-0-0 3-0-1 3-0-1 3-0-1
4 4 4 4 4
0-0-4 4-0-0
4 4 --28 ---
25
Code
5th Semester
Page
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
Module-I
PBT5I101
5th Semester
GENETIC ENGINEERING
5TH SEM
Basic principle of DNA isolation and purification; Restriction endonuclease, Ligase and other modifying enzymes; DNA& RNA Markers, Linker, Adapter and MCS; Gene cloning vectorsPlasmid, bacteriophage, cosmid, BAC, YAC; Expression vectors: basic concept, bacteria and yeast based expression vector; Gene library- genomic and c-DNA, contig library; Polymerase Chain reaction,
Module-II
Basic concept of gene cloning; Cloning of interacting gene: two hybrid and three hybrid assay; Cloning of differentially expressed gene; DNA micro arrays and Chips - principle and manufacturing process; DNA finger printing and DNA foot printing; DNA Sequencing; Site directed mutagenesis; Expression of heterologous gene; In vitro transcription and translation; Gene knock out strategies; RNA interference: Antisense RNA, si RNA and mi RNA; Ribozyme Technology.
Module-III
Molecular markers- Types (RFLP, RAPD, AFLP, SCAR, SSR, SNP, EST), Principle and methodology; Application of molecular markers: in diagnostics, gene tagging, gene mapping, Physical mapping of the genome. Genome analysis using 16S rRNA typing/ sequencing, Genome projects: Human, Rice; Gene therapy and its applications; DNA vaccines and rDNA products; Genetic engineering regulations and safety guidelines.
Text Books:
1. H S Chawla, Plant Biotechnology, Oxford University Press 2. Genetic Engineering, Smita Rastogi & Neelam Pathak, Oxford University Press 3. T A Brown, Gene cloning and DNA Analysis, Black well publishing 4. Primerose et al., Principle of gene manipulation and genomics, Black well publishing. Reference
Page
26
1. Sambrooks et al., Molecular cloning (Vol.I,II,II), CSH Press. 2. Spurr N & Young TD, ICRF Hand book of genome analysis, Blackwell
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
PBT5I102
5th Semester
PLANT AND ANIMAL BIOTECHNOLOGY
Module-I: Plant Biotechnology
Concept of totipotency and plasticity of plant cell, Tissue culure media- preparation, composition and plant growth regulators, Initiation and establishment of culture: Explant preparation, Callus culture, Single cell culture, Suspension culture, Microspore culture Micropropagation: Organogenesis, Somatic embryogenesis, Artificial seed; Protoplast technology: Isolation and culture of protoplast, Somatic hybridization, Screening and selection of somatic hybrid. Secondary metabolites of plant origin and its type; Production of secondary metabolites through tissue culture, Factors affecting the production and its optimization, Bioreactor based production of secondary metabolites and its kinetic studies, isolation and purification of secondary metabolites, Biotransformation with case studies.
Module-II: Animal Biotechnology
Equipments and materials for animal cell, culture technology, Brief discussion on the chemical, physical and metabolic functions of different constituents of culture medium, Development of primary culture. Development of cell line by enzymatic disaggregation, Culture media and growth conditions. Cell growth characteristics and kinetics, Measurement of viability and cytotoxicity; Biology and characterization of the cultured cells, measuring parameters of growth
Module-III: Techniques and applications
Direct transformation (Gene gun, Electroporation, Microinjection, etc.) Concept of genetic transformation: Vector based (Agrobacterium, Virus) and Application of genetic transformation: promoter tagging, activation tagging, herbicide resistance, insect resistance, disease resistance, molecular farming, terminator seed technology; Products of genetic transformation: Case studies for golden rice, Bt cotton and Flavr Savr tomato. Cell transformation, Application of animal cell culture, stem cell cultures, embryonic stem cells and their applications. Hybridoma technology, Organ culture technology, Transfection of animal cells, Future tissue engineering, animal cloning
Text Books:
H S Chawla, Plant Biotechnology, Oxford University Press. K G Ramawat, Plant Biotechnology, S. Chand & Co. A Kumar and SK Sopory, Recent advances in Plant Biotechnology, I.K. International. A Slater et al., Plant Biotechnology, Oxford Univ. Press
Page
1. R. Ian Freshney, Culture of Animal Cells, 3rd Edition, Wiley-Liss publication 2. Martin Clynes, (Eds) Animal Cell culture Techniques Springer Publication 3. Balasubramanian, Bryce, Dharmalingam, Green and Jayaraman (Eds.),Concepts in Biotechnology, University Press, 1996. 4. Hood L.E., Weissman I., Wood W.B. & Wilson J.H. Immunology,Benjamin Cummings, 1989. 5. Biotol Series – Butterworth and Heineman, Oxford, 1992 6. A Text Book of Biotechnology R C Dubey, S Chand publication 7. Animal cell culture by R.I. Freshney 8. Animal Biotechnology by P.Ramadas 9. In vitro cultivation of Animal cells by Dr.C.K.Leach,Butterworth and Heinnmamm Ltd.1994. 10. Hand book of Animal Husbandry by Gopalakrishnan .
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Text Books:
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
PBT5I103 Module-I:
5th Semester
INDUSTRIAL MICROBIOLOGY AND ENZYME TECHNOLOGY
Microbial Processes and fermentation technology: Introduction to fermentation technology, Microbial growth and product formation kinetics in batch, continuous and feed batch fermentation, Large scale production: submerged, solid and semi-solid fermentation, Microbiological processes for production of organic acids; solvents; antibiotics, enzymes, polysaccharides; lipids; pigments and aroma.
Module-II:
Commercial media and strain development: Media selection and development for industrial production, Isolation, selection, characterization of microorganisms, stock culture, development inocula, strain improvement: induced mutation, over producing decontrolled mutants, genetically engineered strain and fermentation.
Module-III:
Stability of enzyme: Enzyme stabilization by selection and genetic engineering, protein engineering. Application of enzymes in industry, analytical purpose and medical therapy. Application of Biocatalyst, Group transfer redox, Elimination, isomarization and rearrengement, C-C bond cleavage, Reaction environment rebuilding, chemical modification, intramolecular cross linking and immobilization.
Text Books
1. Principle of Fermentation Technology , P.F. Stanbury, A. Whitaker and S.J. Hall, Elsevier 2. Industrial Microbilogy, Prescot and Dunn, 3. Biochemical Engineering and Biotechnology Handbook, Atkinson, B and Marituna, F., The Nature Press, Macmillan Publ. Ltd. 4. Biochemical Engineering Fundamentals, Bailey & Olis. MGH.
PBT5J001
Module-I:
IMMUNOLOGY AND IMMUNOTECHNOLOGY
The origin of Immunology, types of immunity, humoral and cell mediated immunity, Primary and secondary lymphoid organ, antigen, cells of immune system, immunoglobulin and antibodies, Major Histocompatibility Complex (MHC)
Module-II:
Antigen processing and presentation, synthesis of antibody and secretion, Molecular basis of Immunology, Molecular basis of antibody diversity, polyclonal and monoclonal antibody, complement system, antigen-antibody reaction.
Module-III
Immune response and tolerance: Regulation of immune response, immune tolerance, hyper sensitivity, autoimmunity; graft versus host reaction, Immuno- deficiency and immunoproliferate diseases. Dysfunctions of immune system and their modulation, Approaches for correcting immune dysfunction, Vaccinology, Monoclonal antibody technology.
Text Books:
Page
1. Immunology and Immunotechnology, Ashim K. Chakravarty, Oxford University Press 2. Microbiology and Immunology by B K Patnaik, T.C. Kar, H.N. Thatoi, India-Tech publication. New Delhi
28
1. Immunology: Lydyard, P.M., Whelan, A., Fanger, M.W., 1st Ed., Viva Books. 2. Essential Immunology: Roitt, I.M., 9th Ed.(1997) Blackwell Scientific, Oxford, UK. 3. Immunology: Kuby, J. 3rd Ed. (1997) Freeman W. H., oxford. 4. Immunotechnology by A Khan, Pearson Publication Reference:
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
5th Semester
GENETIC ENGINEERING LAB
1. Isolation and Restriction enzyme digestion of bacterial genomic DNA 2. Purification of digested DNA-column purification 3. Preparation of target DNA by linker/adapters/alkaline phosphatase treatment for cloning 4. Ligation of DNA fragment with cloning vector 5. Preparation of competent cells 6. Transformation in E.coli with recombinant vector 7.Isolation of recombinants and confirmation of insert DNA in vector 8. Preparation of DNA probe by nick translation /PCR 9. Amplification of DNA sample by PCR 1. Southern Hybridisation 2. Western Hybridisation 3. Expression of cloned gene. 4. DNA profiling by RAPD
PLANT AND ANIMAL BIOTECHNOLOGY LAB PLANT BIOTECHNOLOGY
1. Media preparation, sterilization, explant preparation and establishment of meristem culture 2. Study of organogenesis and multiple shoot generation 3. Somatic embryogenesis in carrot and encapsulation somatic embryo or PLBs 4. Anther culture of Datura 5. Establishment of suspension culture 6. Agrobacterium mediated transformation (Co-cultivation & GUS expression)7. Embry/Endosperm Culture 8. Isolation of protoplasts 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.
ANIMAL BIOTECHNOLOGY
Preparation of culture media and sterilization Organ culture. Fibroblast culture. Adaptation of Animal virus in cell lines BHK-21-vero cell line. Study of effect of anti cancer agent in cell culture. MTT Assay Live cell counting Leukocyte culture Culturing of spleen cells Myeloma cell culture Fusion of cells by PEG
Page
1. Isolation of high yielding microbial strains for the production of commercially important enzymes. 2. . Partial purification of isolated enzymes. 3. Preparation of starter culture of yeast in grape juice 4. Submerged fermentation in single-culture, co-culture condition 5. Production and optimization microbial metabolites (Ethanol or Citric acid) and study of fermentation kinetics 6. Analysis of alcohol or citric acid by spectrophotometric method and gravimetric method. 7. Fermenter operation and study of product formation by batch culture.
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INDUSTRIAL MICROBIOLOGY AND ENZYME TECHNOLOGY LAB INDUSTRIAL MICROBIOLOGY
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
5th Semester
ENZYME TECHNOLOGY LABORATORY:
1. Enzyme assay techniques (e.g Alkalne phosphatase / Amylase / Invertase / dehydrogenases) using whole cells and/ or purified enzyme; Kinetic studies and determination of Km and Vmax 2. Effect of inhibitors on enzyme kinetics 3. Immobilization of cells and enzymes using different matrices (PVA, alginate, etc.) 4. Application of immobilized cells and enzymes in batch and continuous stirred tank reactors. 5. Biotransformation reaction using whole cell and/or enzyme(s). 6. Formulation of enzyme stability. 7. Method of checking the purity of the enzyme -SDS-PAGE 1. 2. 3. 4.
5. 6. 7. 8. 9.
SE GENETIC ENGINEERING AND PLANT BIOTECHNOLOGY LAB
Media preparation and sterilization Callus propagation of medicinal plants and woody plants. Cell suspension culture Isolation and purification of active compounds from plants by column chromatography technique Electroporation Agrobacterium mediated transformation Developing RFLP maps Southern hybridization. Strain improvement techniques- physical, chemical and genetic manipulation methods.
PBT5D001
Module-I:
HONORS: GENOMICS, PROTEOMICS AND METABOLOMICS
Introduction to genomics: Orientation and structure of genomes, subdividing the genome, assembling a physical map of a genome. Sequencing methods and strategies, genome annotation and information from web, bioinformatics Genome sequencing projects- Microbes, plants and animals; Accessing and retrieving genome project Reverse genetics, Structural genomics, Functional genomics and Comparative genomics; High throughput screening in genome for drug discovery-identification of gene targets, Pharmaco-genomics and drug development.
Module-II:
Mapping protein interaction and applications: Global expression profiling, comprehensive mutant libraries, mapping protein interactions, applications of genome analysis and genomes. Introduction and tools of proteomics: Proteomics and Proteomes, Various tools used in proteomics (N-terminal sequencing of proteins, 2-D electrophoresis Differential display proteomics, Yeast two hybrid and three hybrid system, phage display, isoelectrofocusing, Peptide fingerprinting. LC/MS-MS for identification of proteins and modified proteins, SAGE, Protein micro array). Applications of proteomics: Mining proteomes, protein expression profiling, identifying protein – protein Interactions and protein complexes, mapping- protein identification, new directions in proteomics, structural proteomics; Proteomics and Drug delivery. Transcriptomics.
Page
Metabolite isolation and analysis by Mass Spectrometry, Sample preparation (fractionation, enrichment, derivatization), metabolite library, Profiling based on NMR, LIF, LC-UV, 2-D and high (spatial) resolution metabolite profiling, Quantitative metabolomics Metabolite analysis and biochemical pathways: Carbon pathway, Secondary metabolism, amino acid metabolism, Engineered metabolism, Systems biology: Databases (Metabolic pathways resources) and pathway reconstruction.
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Module-III:
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
5th Semester
Texts / References Book:
Page
31
1. Voet D, Voet JG & Pratt CW, Fundamentals of Biochemistry, 2nd Edition. Wiley 2. Brown TA, Genomes, 3rd Edition. Garland Science 3. Campbell AM & Heyer LJ, Discovering Genomics, Proteomics and Bioinformatics, 2nd Edition. Benjamin Cummings 4. Glick BR & Pasternak JJ, Molecular Biotechnology, 3rd Edition, ASM Press 5. Pennington SR & Dunn MJ, Proteomics, Viva publications 6. H.D.Kumar, Molecular Biology, 2nd edition, Vikas Publishing House Pvt. Lt. 7. Singer, M, and Berg.P – Genes and genomes, Blackwell Scientific Publication,Oxford,1991. 8. Beebe.T, and Burke. T,Gene Structure and Transcription, 2nd edition, 1992, Oxford Univ Press. 9. Introduction to Proteomics by Daniel. C. Liebler, Humana press, 2002,198 pages. 10. Principles of gene manipulation and genomics by Primrose, S.B. and Twyman, R.M., Blackwell Publishing (2006) 11. Introduction to Genomics by Lesk AM, Oxford University Press (2008) 12. Proteomics: from protein sequence to function by Pennington, S.R. and Dunn, M. J., Viva Books (2001)
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
6th Semester
Sixth Semester
PC
Bioreactor Design & Analysis Bioinfomatics Stem Cell Engineering/ Molecular Modeling & Drug Designing Biomaterials/Nanobiote chnology Environmental Science & Engineering Industrial Lecture # Business Communication & Skill for Interview # # Yoga
PC PE
PE MC & GS OE HS
MC Total Total Marks: 1100 Total Credits: 24 Honours Protein Engineering And Structure Analysis Minor Bio-Informatics / Bio-Reactor Design and Analysis
Practical
Hours/W eek L/T 3-0
Credit Theory
University Marks
Internal Evaluation
Hours/ Week L/T 2
Credit Practical
Marks
3
100
50
1
50
3-0 3-1
3 4
100 100
50 50
2
1
50
3-1
4
100
50
3-0
3
100
50
2-0
1
50
3 4
1 2
50 100
19
18
500
300
2 13
1 7
50 300
4
4
100
50
32
Course Name
Page
Theory Code
4-0-0
4
1-0-2
3
4-0-0 4-0-0 0-0-1 3-0-1 3-0-1 4-0-0 4-0-0 4-0-0 4-0-0
4 4 1 4 4 4 4 4 4 --28 ---
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SEMESTER : 6TH -------------1. PBT6D001 HONOURS(CP) PROTEIN ENGINEERING AND STRUCTURE ANALYSIS 2. PBT6E101 HS(CP) BUSINESS COMMUNICATION & SKILL FOR INTERVIEW 3. PBT6G001 MINOR(O4) BIO-INFORMATICS 4. PBT6G002 MINOR(O4) BIO-REACTOR DESIGN AND ANALYSIS 5. PBT6H301 OE(CP) INDUSTRIAL LECTURE # 6. PBT6I101 PC(CP) BIOREACTOR DESIGN & ANALYSIS 7. PBT6I102 PC(CP) BIOINFOMATICS 8. PBT6J001 PE(O1) STEM CELL ENGINEERING 9. PBT6J002 PE(O1) MOLECULAR MODELLING & DRUG DESIGNING 10. PBT6J003 PE(O2) BIOMATERIALS 11. PBT6J004 PE(O2) NANOBIOTECHNOLOGY
6th Semester
Page
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
PBT6I101
6th Semester
BIOREACTOR DESIGN & ANALYSIS
Module- I: Principles and concepts
Recapitulation of the principles of Kinetics for chemical and Bio-chemical Reactions. Fundamentals of homogeneous reactions for batch / semi-batch, plug low reactor (PFR), continuous stirred rank reactors (CSTR), fluidized bed reactor bubble column, air lift fermenter etc, stirred tank/mixed reactors., adiabatic and programmed reactors. Unconventional bioreactors: Hollow fiber reactor, membrance reactor, perfusion reactor for animal and plant cell culture.
Module- II: Bioreactor Analysis
Analysis of ideal bioreactors: Fed-Batch reactors, Enzyme catalyzed reactions in CSTRs, CSTR reactors with Recycle and wall growth, Ideal Plug-Flow Tubular reactor. Analysis of Nonideal Reactor Analysis: Concept of ideal and non-ideal reactor; residence time distribution; models of non-ideal reactors – plug flow reactor for microbial processes; Mass transfer in biochemical processes; Multiphase bioreactors – packed bed with immobilized enzymes or microbial cells; three – phase fuidized bed trickling bed reactor; Design and analysis of the above reactor systems; Gas liquid reactors, Reactor stability.
Module- III: Bioreactor Design
Design considerations: oxygen transfer, heat transfer, rheology, mixing. Scale up and scale down concepts. Bioprocess control and computer coupled bioreactors; Growth and product formation by recombinant cells. Mechanical fittings in a bioreactor: vessel, agitation system materials, welds, finish, valves, piping and valves for biotechnology. Instrumentation and control of bioprocesses: Bioreactor sensor, online sensors for cell properties, off-line analytical methods; Biosensors. Bioreactor design calculation.
Text Books 1. 2. 3. 4. 5. 6. 7.
Levenspiel, O., Chemical Reaction Engineering, Wiley Eastern Ltd. Bailey & Olis, Biochemical Engg. Fudamentals, MGH.,1990 Atkinson, B.,Biological Reactors, pion Ltd., London,1974. Coulson, Richardson, Sinnott, An introduction to chemical engineering design, Pergamon Alba S., Humphrey E and Milli N.R., “Bio Chemical Engineering” Academic Press, 1973. Scragg.A.H “Bioreactors in Biotechnology”- A Practical approach Bailey and Ollis, “Biochemical Engineering Fundamentals”, McGraw Hill (2nd Ed.). 1986. Press. 8. Lydersen, D’Elia, Nelson, Bioprocess engineering: Systems and equipment.
Page
34
BIOREACTOR DESIGN & ANALYSIS LAB (UNDER REVISION)
1. Bioreactor operation – Demonstration 2. Batch, fed batch and continuous cultures a) Estimation of Monod parameters b) Pure and mixed cultures. 3. Temperature effect on growth-estimation of energy of activation and Arrhenius constant for micro-organisms. 4. Determination of Oxygen transfer rate KLa determination by sulphite oxidation method KLa determination by dynamic gassing method KLa determination by power correlation analysis 5. Packed bed bioreactor: study of process parameters 6. Fluidised bed reactor: study of process parameters 7. Screening of process variables single dimensional search, Blackett Burman design, design expert etc. 8. Study of rheology of fermentation broth and power determination. 9. Bioprocess control using software 10. Production of secondary metabolites by feed batch culture.
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
PBT6I102
6th Semester
BIOINFORMATICS
Module-I:
Searching Biological Data From databases: Finding the information stored and its retrieval methods at NCBI, EMBL and DDBJ, Protein Data Bank, CSD, Uniprot, PIR, SwissProt, TrEMBL, SNP, Metabolic pathways databases KEGG, MetaCyc, Domain classification databases: SCOP, CATH, Pfam Retrieving microbial and viral genome information.
Module-II:
Sequence alignment and analysis: Local and global alignment, Gap penalty and substitution matrix, Pairwise and Multiple sequence alignment, Fast Alignment method, dynamic programming, Sequence profile and HMM, Basic algorithms for prediction of ORF, promoters, splice sites
Module-III:
Molecular modelling and drug design: Homology modelling, Molecular mechanics and force fields, molecular dynamics simulation, Drug design Process, drug like Property of a molecule, target identification, Docking methods, Basic idea about Molecular descriptors and QSAR analysis.
Text Books:
1. Mount DW, Bioinformatics: Sequence and Genome Analysis, Spring Harbor Press 2. Arthur Lesk, Introduction to Bioinformatics, Oxford University Press. 3. Baxevanis AS and Ouellette BF, Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins, Wiley International Science. 4. Bryan Bergeron, Bioinformatics computing, Prentice Hall Inc 5. Bernhard houbold ,Thomas Wiehe,Introduction to computational biology : an evolutionary approach Blkhauser verlag press
Reference:
1. Tao Jiang, Ying Xu, Michael Q. Zhang, Current Topics in Computational Molecular Biology, MIT press. 2. Thomas lengauer,Bioinformatics from genome to drug .WILLEY-VCH press.
Page
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BIOINFORMATICS LAB
1. Retrieving Human genome data, OMIM, SNP databases to understand genetic and metabolic disorders. (At least 2 each) 2. Mining genomic data to identify genomic features: codon usage, repeats, Homologous sequences etc. 3. Making Phylogenetic tree of given sequences by using ClustalW and PHYLIP. 4. Gene and promoter prediction for Prokaryotes and eukaryotes (comparative analysis by using different tools: at least 3) 5. Learning about molecule visualisation software like Rasmol, Pymol etc. 6. Primary Structural databases: pdb, ndb, csd and Derived databases of structures: DSSP, FSSP, CATH & SCOP. 7. Prediction of secondary structures of proteins: at least 3 methods 8. Prediction of Tertiary structure of proteins and Validation of model protein structure: Energy minimization, Procheck, verify 3D, Prosa II, ERRAT etc. 9. Molecule drawing. Conversion of 2D structure to 3D structure. 10. Molecular docking and analysis of receptor with ligand 11. Molecular Dynamics simulation
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
6th Semester
STEM CELL ENGINEERING/ MOLECULAR MODELING AND DRUG DESIGNING PBT6J001 STEM CELL ENGG Module-1
Stem cell basics: Unique properties of stem cells, embryonic stem cells, adult stem cells, umbilical cord stem cells, similarities and differences between embryonic and adult stem cells. Properties of stem cells, pluripotency, totipotency. Embryonic stemcell: In vitro fertilization, culturing of embryos-isolation of human embryonic stem cells, blastocyst, inner cell mass, growing ES cells in lab, laboratory tests to identify ES cells, stimulation ES cells for differentiation, properties of ES cells.
Module-II
Adult stem cells: Somatic stem cells, test for identification of adult stem cells, adult stem cell differentiation, trans differentiation, plasticity, different types of adult stem cells. Stem cell in drug discovery and tissue engieering: Target identification, Manipulating differentiation pathways, stem cell therapy Vs cell protection, stem cell in cellular assays for screening stem cell techniques: fluorescence activated cell sorting (FACS), time lapse video, green fluorescent protein tagging, stem cell based drug discovery, drug screening and toxicology.
Module-III
Genetic engineering and therapeutic application of stem cells: Gene therapy, genetically engineered stem cells, stem cells and Animal cloning, transgenic animals and stem cells, Therapeutic applications Parkinson disease, Neurological disorder, limb amputation, heart disease, spinal cord injuries, diabetes, burns, HLA typing, Alzheimer’s disease, tissue engineering application – production of complete organ, kidney, eyes, heart, brain. Stem cell regulations, debate, social and ethical concerns
Books
Page
36
1. Embryonic Stem cells by Kursad and Turksen. 2002.Humana Press. 2. Stem cell and future of regenerative medicine. By committee on the Biological and Biomedical applications of Stem cell Research.2002.National Academic press 3. Hematopietic Stem Cell Transplantation by Treleaven, J., first edition 2009 4. Essentials of Stem Cell Biology by Lanza, R., second Edition, 2009 Academic Press 5. Molecular Cell Biology by Lodish et al., sixth Ed., W.H. Freeman & Co. 2008 6. Stem Cells: From Bench to Bedside by Bongso and Ariff BTBT903 Nanobi
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
PBT6J002
6th Semester
MOLECULAR MODELING AND DRUG DESIGNING
Module-I:
Introduction to Molecular Modelling and its applications Biomolecular modeling problems: protein folding, protein misfolding, nucleic acid/ protein interactions, and RNA folding. Basic concepts of quantum mechanics, ab initio, semi-empirical and density functional theory calculations, Molecular size versus accuracy. Approximate molecular orbital theories. Molecular mechanisms, energy calculations, Bond stretch, Angle bending, torsional terms, Electrostatic interaction- Van der waals interactions. Miscellaneous interactions.
Module-II:
Introductionto molecular dynamics and simulations; Molecular Dynamics using simple models; Dynamics with continuous potentials, Constant temperature and constant dynamics; Conformation searching and systematic search; Monte-carlo simulation of biomolecules and bio-polymers. Comparative modeling of protein: by homology- the alignment, construction of frame work, selecting variable regions, side chain placement and refinement, validation of protein models – Ramchandran plot, threading and ab initio modeling.
Module-III:
Analog based drug designing : Introduction to QSAR. lead module, linear and nonlinear modeled equations, biological activities, physicochemical parameter and molecular descriptors, molecular modelling in drug discovery. Structure based drug designing: 3D pharmacophores, molecular docking, De novo Ligand design, Free energies and solvation, electrostatic and non-electrostatic contribution to free energies. 3D data base searching and virtual screening, Sources of data, molecular similarity and disimilarity searching, combinatorial libraries – generation and utility.
Text Book: 1. 2.
A R Leach, Principles and applications of modeling, Prentice Hall. Hans Pieter, Heltje & Gerd Folkens, Molecular Modelling, VCH.
References:
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37
1. Jonathan Good man, Chemical Applications of Molecular Modelling, Cambridge Press
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
6th Semester
BIOMATERIALS / NANOBIOTECHNOLOGY PBT6J003 BIOMATERIAL Module-I:
Introduction to biomaterials, Structure and properties (mechanical, thermal, optical, electrical and surface) of biomaterials, Synthetic polymer, Biopolymer
Module-II
Novel Biomaterials and uses in engineering and tissue engineering: Hydrogels, self-assembling peptides. Implants materials: metallic implant materials, stainless steels, Co-based alloys, Tibased alloys; ceramic implant materials, aluminum oxides, hydroxyapatite glass ceramics carbons. Polymeric implant, Polymers for drug delivery: types of polymer, pharmaceutical polymers, physicochemical properties of polymers and relationship with structure, properties, kinetics, mechanisms and applications.
Module- III:
Biocompatibility and blood compatibility, Biomaterials: its foreign body response in a body. Biological interface, interaction with biomaterials and adhesion, Biological response to implants, 2D and 3D matrices (scaffolds) of biomaterials for tissue engineering, Soft tissue and hard tissue replacement, cardiovascular implants, Biomaterials for ophthalmology, orthopaedic and dental implants, Biologically functional biomaterials, Testing and clearance of biomaterials, Evaluation of biomaterials.
Text Books
Page
38
1. Buddy D. Ratner Allan S. Hoffman Frederick J. Schoen Jack E. Lemons. Biomaterials Science, Second Edition: Wiley Science 2004. 2. Bhatt SV, Biomaterial, Narosa publishing house 3. Park J and R. S. Lakes R S, Biomaterials: An Introduction, Springer 2009
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
PBT6J004
6th Semester
NANOBIOTECHNOLOGY
Module-I
Concept of Nano-biotechnology & Historical background. Fundamental sciences and broad areas of Nanobiotechnology, Various applications of Nano-biotechnology, biology at the nano interface, Cell – Nanostructure interactions, Science of Self-assembly and self organization from,
Module-II
Structural and functional principles of nanobiotechnology. Protein-based Nanostructures: Nanomotors and DNA based nanostructures. Tools of measurement: Scanning probe techniques (SPM), Electron Microscopy. Introduction to Nanostructures: Fullerenes, Quantum Dots and Metal-based Nanostructures, nanopolymers. Carbon nanotubes, nanoparticles and nanowires. Microbial synthesis of nanoparticles, magnetosomes, bacteriorhodopsin. Micro- and nano – fabrication: Chemical Vapor Deposition, Photolithography, Features of Nanoscale Growth, Micromachining: MEMS; BioMEMS, microarray technology,
Module-III
Microfabricated devices, Nanoanalysis and nanobiosensors; Lab-on-a-chip devices and their potential in nanobiotechnology. Medical Applications of Nanobiotechnology: Polymeric nanocontainers for drug delivery and gene delivery, imaging applications, Nanoparticles’ Cytotoxicity
Text Book:
Niemeyer C M and Mirkin C A, Nanobiotechnology: Concepts, Applications and Perspectives, Wiley VCH, 2004 2. Chattopadhyaya KK and Banerjee AN, Nanoscience and Nanotechnology, PHI learning Pvt. Ltd. 3. Bionanotechnology by David S.Goodsell, 2004, Wiley Publications. Pages-337.
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1.
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
6th Semester
PMG6M001 ENVIRONMENTAL SCIENCE AND ENGINEERING (3-0-0)
Page
Module II Ecosystems Concept of an ecosystem. Structure and function of an ecosystem. Producers, consumers and decomposers. Energy flow in the ecosystem. Ecological succession. Food chains, food webs and ecological pyramids. Introduction, types, characteristic features, structure and function of the following ecosystem :a) Forest ecosystem b) Grassland ecosystem c) Desert ecosystem d) Aquatic ecosystems (ponds, streams, lakes, rivers, oceans, estuaries) Environmental Pollution Definition Cause, effects and control measures of :a) Air pollution b) Water pollution c) Soil pollution d) Marine pollution e) Noise pollution f) Thermal pollution g) Nuclear hazards Solid waste Management: Causes, effects and control measures of urban and industrial wastes. Role of an individual in prevention of pollution.
40
Module I Multidisciplinary nature of environmental studies Definition, scope and importance, Need for public awareness. Natural Resources: Renewable and non-renewable resources: Natural resources and associated problems. a) Forest resources: Use and over-exploitation, deforestation, case studies. Timber extraction, mining, dams and their effects on forest and tribal people. b) Water resources: Use and over-utilization of surface and ground water, floods, drought, conflicts over water, dams-benefits and problems. c) Mineral resources: Use and exploitation, environmental effects of extracting and using mineral resources, case studies. d) Food resources: World food problems, changes caused by agriculture and overgrazing, effects of modern agriculture, fertilizer-pesticide problems, water logging, salinity, case studies. e) Energy resources: Growing energy needs, renewable and non renewable energy sources, use of alternate energy sources. Case studies. f) Land resources: Land as a resource, land degradation, man induced landslides, soil erosion and desertification. Role of an individual in conservation of natural resources. Equitable use of resoureces for sustainable lifestyles.
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
6th Semester
Pollution case studies. Disaster management: floods, earthquake, cyclone and landslides. Module III Social Issues and the Environment From Unsustainable to Sustainable development Urban problems related to energy Water conservation, rain water harvesting, watershed management Resettlement and rahabilitation of people; its problems and concerns. Case Studies Environmental ethics : Issues and possible solutions. Climate change, global warming, acid rain, ozone layer depletion, nuclear accidents and holocaust. Case Studies. Wasteland reclamation. Consumerism and waste products. Environment Protection Act. Air (Prevention and Control of Pollution) Act. Water (Prevention and control of Pollution) Act Wildlife Protection Act Forest Conservation Act Issues involved in enforcement of environmental legislation. Public awareness.
Page
References 1. Agarwal, K.C. 2001 Environmental Biology, Nidi Publ. Ltd. Bikaner. 2. R. Rajagopalan, Environmental Studies, Oxford University Press. 3. Ajith Sankar, Environmental Management, Oxford University Press. 4. Bharucha Erach, The Biodiversity of India, Mapin Publishing Pvt. Ltd., Ahmedabad – 380 013, India, Email:
[email protected] (R) 5. Brunner R.C., 1989, Hazardous Waste Incineration, McGraw Hill Inc. 480p 6. Clark R.S., Marine Pollution, Clanderson Press Oxford (TB) 7. Cunningham, W.P. Cooper, T.H. Gorhani, E & Hepworth, M.T. 2001, Environmental Encyclopedia, Jaico Publ. House, Mumabai, 1196p 8. De A.K., Environmental Chemistry, Wiley Eastern Ltd. 9. Down to Earth, Centre for Science and Environment (R)
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Module IV Human Population and the Environment Population growth, variation among nations. Population explosion – Family Welfare Programme. Environment and human health. Human Rights. Value Education. HIV/AIDS. Women and Child Welfare. Role of Information Technology in Environment and human health. Case Studies.
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
6th Semester
PEN6E101 BUSINESS COMMUNICATION AND SKILL FOR INTERVIEW Course Objectives To develop communication competence in prospective engineers. To enable them to convey thoughts and ideas with clarity and focus. To develop report writing skills. To equip them to face interview & Group Discussion. To inculcate critical thinking process. To prepare them on problem solving skills. To provide symbolic, verbal, and graphical interpretations of statements in a problem description. To understand team dynamics & effectiveness. To create an awareness on Engineering Ethics and Human Values. To install Moral and Social Values, Loyalty and also to learn to appreciate the rights of others. To learn leadership qualities and practice them. MODULE I Communication Skill: Introduction to Communication, The Process of Communication, Barriers to Communication, Listening Skills, Writing Skills, Technical Writing, Letter Writing, Job Application, Report Writing, Non-verbal Communication and Body Language, Interview Skills, Group Discussion, Presentation Skills, Technology-based Communication. MODULE II Critical Thinking & Problem Solving: Creativity, Lateral thinking, Critical thinking, Multiple Intelligence, Problem Solving, Six thinking hats, Mind Mapping & Analytical Thinking. Teamwork: Groups, Teams, Group Vs Teams, Team formation process, Stages of Group, Group Dynamics, Managing Team Performance & Team Conflicts. MODULE III Ethics, Moral & Professional Values: Human Values, Civic Rights, Engineering Ethics, Engineering as Social Experimentation, Environmental Ethics, Global Issues, Code of Ethics like ASME, ASCE, IEEE.
Page
Expected outcome: The students will be able to Communicate effectively. Make effective presentations. Write different types of reports. Face interview & group discussion. Critically think on a particular problem. Solve problems.
42
MODULE IV Leadership Skills: Leadership, Levels of Leadership, Making of a leader, Types of leadership, Transactions Vs Transformational Leadership, VUCA Leaders, DART Leadership, Leadership Grid & leadership Formulation.
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
6th Semester
Work in Group & Teams Handle Engineering Ethics and Human Values. Become an effective leader. References: 1. Barun K. Mitra; (2011), “Personality Development & Soft Skills”, First Edition; Oxford Publishers. 2. Kalyana; (2015) “Soft Skill for Managers”; First Edition; Wiley Publishing Ltd. 3. Larry James (2016); “The First Book of Life Skills”; First Edition; Embassy Books. 4. Shalini Verma (2014); “Development of Life Skills and Professional Practice”; First Edition; Sultan Chand (G/L) & Company 5. John C. Maxwell (2014); “The 5 Levels of Leadership”, Centre Street, A division of Hachette Book Group Inc.
PBT6D001
PROTEIN ENGINEERING AND ANALYSIS (HONORS)
Module- I
Overview of protein structure and its hierarchical architecture; Protein engineering – definition, applications; Forces stabilizing proteins – Van der waals, electrostatic, hydrogen bonding and weakly polar interactions, hydrophobic effects. Structural features of protein, Ramachandran map, Protein-protein, Protein-DNA, protein-ligand interactions. Protein structure-function relationship. Stability of Protein Structure: Laws of thermodynamics, heat, energy and work, chemical equilibrium flexibility, reversible folding and unfolding, pH titration, chemical denaturation, thermal denaturation, solvent perturbation and chemical modification,
Module-II
Features or characteristics of proteins that can be engineered- affinity and specificity; Experimental methods of protein engineering: Rational designing, Directed evolution like site directed mutagenesis, Module shuffling, Guided protein recombination, etc.; Computational approaches to protein engineering. Mechanism of stabilization of proteins from psychrophiles and thermophiles vis-à-vis those from mesophiles; Protein and enzyme engineering case studies for its stability, specifity and affinity- Protease, Lipase and Lysozyme. Role of solvent.
Module-III
Characterization of proteins: NMR spectroscopy, crystallography, spectroscopic (UV-Vis, CD, IR, Florescence), calorimetric methods, Viscometry, Molecular sieve chromatography, electrophoresis, EPR in protein structure and function analysis with example.
Page
6. L. Alberghina, Protein Engineering for industrial biotechnology, Harwood Academic Publisher
43
Text Books /References: 1. Edited by T E Creighton, Protein structure: A practical approach, 2nd Edition, Oxford press. 2. Edited by T E Creighton, Protein function. A practical approach, 2nd Edition, Oxford university press. 3. Edited by T E Creighton, Protein function. A practical approach. Oxford university press. 4. Cleland and Craik, Protein Engineering, Principles and Practice, Vol 7, Springer Netherlands. 5. Mueller and Arndt., Protein engineering protocols, 1st Edition, Humana Press.
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
6th Semester
PBT6G001 BIOINFORMATICS (MINOR) Module-I:
Searching Biological Data From databases: Finding the information stored and its retrieval methods at NCBI, EMBL and DDBJ, Protein Data Bank, CSD, Uniprot, PIR, SwissProt, TrEMBL, SNP, Metabolic pathways databases KEGG, MetaCyc, Domain classification databases: SCOP, CATH, Pfam Retrieving microbial and viral genome information.
Module-II:
Sequence alignment and analysis: Local and global alignment, Gap penalty and substitution matrix, Pairwise and Multiple sequence alignment, Fast Alignment method, dynamic programming, Sequence profile and HMM, Basic algorithms for prediction of ORF, promoters, splice sites
Module-III:
Molecular modelling and drug design: Homology modelling, Molecular mechanics and force fields, molecular dynamics simulation, Drug design Process, drug like Property of a molecule, target identification, Docking methods, Basic idea about Molecular descriptors and QSAR analysis.
Text Books:
6. Mount DW, Bioinformatics: Sequence and Genome Analysis, Spring Harbor Press 7. Arthur Lesk, Introduction to Bioinformatics, Oxford University Press. 8. Baxevanis AS and Ouellette BF, Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins, Wiley International Science. 9. Bryan Bergeron, Bioinformatics computing, Prentice Hall Inc 10. Bernhard houbold ,Thomas Wiehe,Introduction to computational biology : an evolutionary approach Blkhauser verlag press
Reference:
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3. Tao Jiang, Ying Xu, Michael Q. Zhang, Current Topics in Computational Molecular Biology, MIT press. 4. Thomas lengauer,Bioinformatics from genome to drug .WILLEY-VCH press.
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
6th Semester
PBT6G002 BIOREACTOR DESIGN & ANALYSIS (MINOR) Module- I: Principles and concepts
Recapitulation of the principles of Kinetics for chemical and Bio-chemical Reactions. Fundamentals of homogeneous reactions for batch / semi-batch, plug low reactor (PFR), continuous stirred rank reactors (CSTR), fluidized bed reactor bubble column, air lift fermenter etc, stirred tank/mixed reactors., adiabatic and programmed reactors. Unconventional bioreactors: Hollow fiber reactor, membrance reactor, perfusion reactor for animal and plant cell culture.
Module- II: Bioreactor Analysis
Analysis of ideal bioreactors: Fed-Batch reactors, Enzyme catalyzed reactions in CSTRs, CSTR reactors with Recycle and wall growth, Ideal Plug-Flow Tubular reactor. Analysis of Nonideal Reactor Analysis: Concept of ideal and non-ideal reactor; residence time distribution; models of non-ideal reactors – plug flow reactor for microbial processes; Mass transfer in biochemical processes; Multiphase bioreactors – packed bed with immobilized enzymes or microbial cells; three – phase fuidized bed trickling bed reactor; Design and analysis of the above reactor systems; Gas liquid reactors, Reactor stability.
Module- III: Bioreactor Design
Design considerations: oxygen transfer, heat transfer, rheology, mixing. Scale up and scale down concepts. Bioprocess control and computer coupled bioreactors; Growth and product formation by recombinant cells. Mechanical fittings in a bioreactor: vessel, agitation system materials, welds, finish, valves, piping and valves for biotechnology. Instrumentation and control of bioprocesses: Bioreactor sensor, online sensors for cell properties, off-line analytical methods; Biosensors. Bioreactor design calculation.
Text Books
Levenspiel, O., Chemical Reaction Engineering, Wiley Eastern Ltd. Bailey & Olis, Biochemical Engg. Fudamentals, MGH.,1990 Atkinson, B.,Biological Reactors, pion Ltd., London,1974. Coulson, Richardson, Sinnott, An introduction to chemical engineering design, Pergamon Alba S., Humphrey E and Milli N.R., “Bio Chemical Engineering” Academic Press, 1973. Scragg.A.H “Bioreactors in Biotechnology”- A Practical approach Bailey and Ollis, “Biochemical Engineering Fundamentals”, McGraw Hill (2nd Ed.). 1986. Press. 8. Lydersen, D’Elia, Nelson, Bioprocess engineering: Systems and equipment.
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1. 2. 3. 4. 5. 6. 7.
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
Code
Course Name
GS
Nano Science & Bio Technology Environmental Biotechnology Medical & Pharmaceutical Biotechnology Soft Computing */ Other subjects Advance Lab-II/ Project
PE PE OE PC
Projects on Internet of Things
Seventh Semester Theory
Hours/W eek L/T 3-1
Credit Theory
University Marks
Internal Evaluation
4
100
50
3-1
4
100
50
3-1
4
100
50
3-1
4
100
50
7th Semester Practical
Hours/ Week L/T
Credit Practical
Marks
8
4
200
8
4
200
Page
43
Total 16 16 400 200 16 8 400 Total Marks: 1000 Total Credits: 24 Honours Ethics and IPR in 4 4 100 50 Biotechnology Minor Downstream Process Engineering/ Environmental Technology SEMESTER : 7TH --------------------------------------------------------------------------------------------SL. SUBJECT CATEGORY SUBJECT NAME L-T-P CREDIT NO. CODE -------------------------------------------------------------------------------1. PBT7C001 GS(CP) NANO & BIOSCIENCE 4-0-0 4 2. PBT7D001 HONOURS(CP) ETHICS AND IPR IN BIO-TECHNOLOGY 4-0-0 4 3. PBT7G001 MINOR(O5) DOWNSTREAM PROCESS ENGINEERING 4-0-0 4 4. PBT7G002 MINOR(O5) ENVIRONMENTAL TECHNOLOGY 4-0-0 4 5. PBT7H001 OE(O4) SOFT COMPUTING 4-0-0 4 6. PBT7H002 OE(O4) OTHER SUBJECTS 4-0-0 4 7. PBT7H201 FE(CP) PROJECTS ON INTERNET OF THINGS 0-0-4 4 8. PBT7I201 PC(O3) ADVANCE LAB - II 0-0-4 4 9. PBT7I202 PC(O3) PROJECT 0-0-4 4 10. PBT7J001 PE(CP) ENVIRONMENTAL BIOTECHNOLOGY 4-0-0 4 11. PBT7J002 PE(CP) MEDICAL & PHARMACEUTICAL BIOTECHNOLOGY 4-0-0 4 --28 ---
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
7th Semester 7TH SEM
NANO SCIENCE & BIO TECHNOLOGY
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PBT7C001
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
Module -I:
PBT7G001
7th Semester
DOWNSTREAM PROCESS ENGINEERING
Introduction; An overview of bioseparation. Role and importance of Bioseparation process in biotechnological processes. Problems and requirements of bioproduct purification. Cost- cutting strategies Characteristics of biological mixtures – Process of Classification of Bioproducts Biological activity Analysis of purity-Process economics-Capital and operating cost analysis, Separation of cells and other insolubles from fermented broth. Filtration and microfiltration, centrifugation (batch, continuous, basket).
Module -II:
Cell disruption: Physical methods (osmotic shock, grinding with abrasives, solid shear, liquid shear), Chemical methods (alkali, detergents), Enzymatic methods Ultra-filtration: Semipermeable membranes, membrane geometry and ultrafiltration module configuration. Chromatography in bioseparation.
Module-III:
Separation of soluble bio-products: Liquid-liquid extraction, aqueous two-phase extraction, precipitation, adsorption. Other bioseparation techniques like Dialysis, electro-dialysis, Liquid Electrophoresis. Products polishing : Crystallization and drying.
Text Books: 1. 2. 3. 4.
M.R. Ladisch, Bioseparations Engineering, Wiley Interscience 2001 Kennedy and Cabral, Recovery processes for biological materials. Heinemann, Product Recovery in Bioprocess Technology, Butterworth Publication. Roger G. Harrison, Paul W. Todd, Scott R. Rudge, and Demetri Petrides, Bioseparations Science and Engineering, Oxford University Press, USA (October 31, 2002) 5. Belter PA and Cussler E, “ Bioseparations ”, Wiley 1985 Reference Books
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1. Wankat P.C, “ Rate controlled separations ”, Elsevier, 1990 2. Asenjo J.M., “ Separation processes in Biotechnology ” Marcel Dekker Inc. 1993.
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
Module-I
PBT7G002
7th Semester
ENVIRONMENTAL BIOTECHNOLOGY
Introduction to environmental biotechnology, Environmental monitoring bioreporter, biomarker. Bioprospecting, Biomicroelectronics and biosensor technology Introduction to environmental pollutants: Water, Soil and Air: their sources and effects. Removal of Specific Pollutants: Sources of Heavy Metal Pollution, Microbial Systems for Heavy Metal Accumulation, Biosorption & detoxification mechanisms. Microbiology and biochemistry of waste water treatment: Biological Treatment of anaerobic and aerobic; methanogenesis, methanogenic, acetogenic, and fermentative bacteria- technical process and conditions; Use of Genetically Engineered Organisms. emerging biotechnological processes in waste - water treatment; Applications include treatment of municipal and industrial wastewaters,
Module-II
Biodegradation of xenobiotic compounds: Xenobiotic compounds : Aliphatic, Aromatics, Polyaromatic Hydrocarbons, Polycyclic aromatic compounds, Pesticides, Surfactants and microbial treatment of oil pollution. Biotransformations and biocatalysts: Basic organic reaction mechanism, Common prejudices against Enzymes. Advantages & Disadvantages of Biocatalysts, Isolated Enzymes versus whole cell systems. Mechanistic Aspects and Enzyme Sources. Biocatalytic Application, Catalytic Antibodies; Stoichiometry, kinetics, and thermodynamics of microbial processes for the transformation of environmental contaminants.
Module-III
Biooxidation & microbial leaching: Biooxidation – Direct and Indirect Mechanisms, Recovery of metals from solutions; Microbes in petroleum extraction; Microbial desulfurization of coal. Clean technologies: Composting Technology and Organic farming, biofertilizers, biopesticides, microbial polymer production and bio plastic technology. Biotechnology of fossil fuels: desulfurization of coal, oil shales, microbial enhanced oil recovery (MEOR). Biofuels: Biogas technology, biohydrogen, bioethahnol production. Biotechnology of mineral processing. Ethical issues in environmental biotechnology and regulatory framework.
Text Books
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1. Rittmann B and McCarty P, Environmental Biotechnology Principles and Applications Mc Graw Hill 2001 2. Evans, G.M., Furlong, J C.,” Environmental Biotechnology- Theory and application”, John Wiley & Sons, Ltd, USA. 2003 3. Environmental biotechnology, Scragg Alan, Oxford University Press, 2005 4. Environmental Microbiology, W.D. Grant & P.E. Long, Blakie, Glassgow and London. 5. Microbial Gene Technology, H. Polasa (ED.) South Asian Publishers, New Delhi. 6. Biotreatment Systems, Vol. 22, D. L. Wise (Ed.), CRC Press, INC. 7. Standard Methods for the Examination of Water and Waste Water (14 th Education) , 1985. American Public health Association 8. Environmental Biotechnology by Bruce Rittmann and Perry McCarty 6. Biotransformations : K. Faber (1995), Springer- Verlag.
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
PBT7J002
7th Semester
MEDICAL AND PHARMACEUTICAL BIOTECHNOLOGY
Module-I
Drug Development in Pharmaceutical Process
Module-II
Disease Diagnosis and Therapy
Module III
Proteomics in Drug Development
Production of pharmaceuticals by genetically engineered cells (hormones, interferon), Microbial transformation for production of important pharmaceuticals (steroids and semi-synthetic antibiotics), Techniques for development of new generation antibiotics, Protein engineering, drug design, drug targeting
ELISA and hybridoma technology, Use of enzymes in clinical diagnosis, Use of biosensors for rapid clinical analysis, Diagnostic kit development for microanalysis, Genetic diseases and DNA based diagnoses, DNA vaccine, Gene Therapy, Toxicogenomics Role of Proteomics in Drug Development, Diagnosis of disease by Proteomics, Separation and identification techniques for protein analysis, Development of antibody based protein array for diagnosis
Text Books
1. Balasubramanian, Bryce, Dharmalingam, Green and Jayaraman(ed), Concepts in Biotechnology, University Press, 1996 2. Epenetos A.A.(ed), Monoclonal antibodies: applications in clinical oncology, Chapman and Hall Medical, London 3. Text book of industrial pharmacy by S R Hiremath, Orient Black Swan publication
DOWNSTREAM PROCESS ENGINEERING LAB
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47
1. Harvesting cells using filtration or centrifugation techniques, identification of extracellular products (enzyme / poly saccharide / some other traceable element) from fermentation broth or shake-flask culture broth. 2. Compare the centrifugation of bacterial culture and ethanol precipitated DNA and calculate relative centrifugal force for the two centrifugations 3. Chemical cell disruption and assay for intracellular products 4. Mechanical cell disruption and assay for intracellular products 5. Protein fractionation using precipitation/solvent extraction techniques. 6. Protein fractionation using chromatography (gel filtration and ion-exchange) 7. Separation of proteins and DNA using affinity chromatography 8. Gas chromatography/HPLC
B.Tech (Biotechnolgy) detail Syllabus for Admission Batch 2015-16
PBT7D001
7th Semester
HONORS: ETHICS AND IPR IN BIOTECHNOLOGY
Module-I:
Concept of property, rights, duties and their correlation; Intellectual property rights and its typesPatents, Trademarks, Copyright & Related Rights, Industrial Design, Traditional Knowledge, Geographical Indications, Protection of new GMOs; Process patent vs product patent; International framework for the protection of IP; IP as a factor in R&D; IPs of relevance to Biotechnology and few Case Studies; Introduction to History of GATT, WTO,WIPO and TRIPS.
Module-II:
Basic requirement of a patentable invention- novelty, inventive step, Prior art and Stae of art; Patent databases; Searching International Databases; Analysis and report formation; Indian Patent Act 1970 and Recent Amendments; Filing of a patent application; Precautions before patenting-disclosure/non-disclosure; WIPO Treaties; Budapest Treaty; PCT and Implications; Role of a Country Patent Office; Procedure for filing a patent, International patenting-requirement, Patent infringement- meaning, scope, litigation, remedies; Case studies and examples-Rice, Neem etc.
Module-III:
Introduction to Biosafety regulations; Primary Containment for Biohazards and Biosafety Levels; Biosafety Levels of Specific Microorganisms; Recommended Biosafety Levels for Infectious Agents and Infected Animals; Biosafety guidelines - Government of India. Definition of GMOs & LMOs; Roles of Institutional Biosafety Committee, RCGM, GEAC etc. for GMO applications in food and agriculture; Environmental release of GMOs; Overview of National Regulations and relevant International Agreements including Cartagena Protocol. Concept of Bioethics, Public concerns on Human genome research and transgenics- Genetic testing and screening, Ethics in clinical trials and GCP, ELSI & Human genome projects; Ethics in human cloning-a case study.
Text Book
Page
48
1. Stanley SA, Bioethics, Wisdom educational services 2. Sateesh MK, Bioethics and Biosafety, IK International Pvt. Ltd.
