Q4/6W/04-13 Reg. No

St. Joseph’s College of Arts & Science (Autonomous) St. Joseph’s College Road, Cuddalore – 607001 ABC401 - BIOPHYSICS Time : 3 hrs Max Marks :75 SECTION – A (25X1=25) Answer ALL Questions I. Choose the correct answer 1. α-helix stabilized by hydrogen bonds between a) NH & CO groups of side chain b) NH & NH groups of same chain c) NH & COOH groups of all chain d) NH & CO groups of main chain 2. The amino acids with longest side chain is a) Argine b) Lysine & leucine c) Arginine & lysine d) Asparagine 3. Nucleic acids are polymers of a) Nucleosides b) Phosphorylated nucleosides c) Glycosides d) Peptides 4. Renaturation can be studied by a) Nitro cellulose filters b) Westernblotting d) RIA

c) ELISA

5. Active transport is mediated by a) Channel protein b) carrier protein c) Both depending on concentration of ion d) All the above 6. The most important function of cell wall is a) Shape and mechanical strength b) Semipermeability c) Elasticity d) Protection

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Q4/6W/04-13 7. Thin elastic selectively permeable membrane is a) Cell membrane b) Cell wall c) Nuclear membrane d) All the above 8. Lipid-Protein sandwitch model for membrane structure was proposed by a) Robertson b) Singer & Nicolson c) Danielli & Davson d) None of the above 9. Protein contain regulatory sites called a) Folding sites b) Complementary surfaces c) Clefts d) Allosteric sites 10. Nitrogenous bases of denatured DNA exhibit which of following phenomenon a) Hypochromism b) Amphichromism c) Hyperchromism d) None of the above II. True or False : 11. Electromagnetic graph is called as ECG. 12. The pH of water is 5.0. 13. Detector do not convert electro magnetic energy. 14. Isotope dilution analysis is used in measurement of body water. 15. “PPO” is primary fluor. III. Answer in One or Two sentences 16. Define nucleotide. 17. What is annealing? 18. What is Denaturation? 19. What are histones? 20. What is Haemoglobin?

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Q4/6W/04-13 21. Define membrane potential. 22. What is radioactive decay? 23. What are chromophores? 24. What is gradient? 25. Define radio active with example. SECTION – B (4X5=20) Answer any FOUR Questions 26. Explain central dogma. 27. Write a note on zwitter ion. 28. What is nerve impulse? Explain. 29. Explain isotope dilution analysis. 30. Give a brief note on Autoradiography. 31. Write a note on applications of radioisotopes. SECTION – C (3X10=30) Answer any THREE Questions 32. Describe Watson crick model & base pairing. 33. Explain primary structure of protein. 34. Discuss the scintillation counter in detail. 35. Explain the a note on conduction of nerve impulse. 36. Explain the principle and application of UV-visible spectrophotometry.

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BIOPHYSICS - 04 13.pdf

Isotope dilution analysis is used in measurement of body water. 15. “PPO” is primary fluor. III.Answer in One or Two sentences. 16. Define nucleotide. 17. What is ...

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