Monitoring of Luteinizing Hormone Receptor in Ovarian of Mice by radioactive iodine labeled Luteinizing Hormone (131I-LH) Injections Imam Mustofa*), Laba Mahaputra*) and Herry Agoes Hermadi**) *)Laboratory of Veterinary Obstetrics , **) Laboratory of Veterinary Sterility Faculty of Veterinary Medicine, Universitas Airlangga Kampus C Unair, Jl. Mulyorejo, Surabaya-60115. Tel. +62-031-5992377, Fax. +62-031-5993015, email : [email protected]

Abstract Fifteen mice were randomly divided into three groups, were injected subcutaneously with a solution of 131I-LH (radiation activity of 100 thousand count per minute) at proestrus, estrous and diestrous, respectively. Necropsy was performed three hours after injection to determined the number of follicles, corpus luteums or corpus albicans, and measured radiation activity. Percent count per minute (cpm ovaries against the solution before it was injected) was used as an indicator of the level of absorption of 131I-LH by the ovaries. The results showed that absorption of 131I-LH per ovary and per unit functional structure (follicles / CLs) highest was at the estrus phase, followed by pro-estrus and lowest in diestrus phase. References Fox, R.R. and. C.W. Laird. 1970. Sexual Cycles. Lea and Febiger. Philadelphia. Hafez, E.S.E. 1970. Reproduction and breeding Techniques for Laboratory Animals. Lea and Febiger. Philadelphia. Hafez, E.S.E. , M.C. Levaseur and C. Thibault. 1980. Folliculogenesis, egg maturation and ovulation. In: Hafez, E.S.E. (Ed) 1980. 4th ed. Reproduction in Farm Animals. Lea and Febiger. Philadelphia. Hunter, R.H.F. 1982. Reproduction in Farm Animals. ELBS Hongkong. Nalbandov, A.V. 1976. Reproduction Physiology of Mammals and Birds. 3rd ed. W.H. Freeman and Company. Sanfransisco.

Retyped from the archive: Media Kedokteran Hewan (MKH) / Veterinary Medicine Journal (Med.vet.J.) Vol.5 No.1. January 1989, page: 1-9. Full text: http://prof_dr_imam-mustofa-fkh.web.unair.ac.id/